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†
Modified from Sambrook, J., Molecular
Cloning: A Laboratory Manual, p. B.23
(1989). See also Current Protocols in
Molecular Biology, p. A.2.1 (1993).
—or—
1X, to yield 89 mM Tris base, 89 mM boric acid, and
2 mM EDTA.
2. 10X Tris-acetate-EDTA (TAE) stock buffer
(0.4 M Tris, 0.2 M acetic acid, 10 mM EDTA,
pH ~8.4, 1000 ml)
Tris base (FW 121.1)
Acetic acid (99.5%)
EDTA solution
(0.5 M, pH 8.0, soln. 3)
Deionized H
O
2
Stir. Do not adjust pH. Dilute to 1X before use to
yield 40 mM Tris base, 20 mM acetic acid, and 1
mM EDTA.
3. EDTA solution (ethylenediamine tetraacetic acid)
(0.5 M, pH 8.0, 100 ml)
Na
EDTA·2H
O, (FW 372.2)
2
2
Deionized H
O
2
NaOH (10 M) to pH 8.0
Deionized H
O
2
Sample loading buffer
Loading buffer
(5X, 25% Ficoll 400, 0.25% Bromophenol blue
Deionized H
O
2
Ficoll 400
Bromophenol blue (FW 691.9)
Deionized H
O
2
Add to sample in proportion so that 1/5 of the final
volume is loading buffer. (Loading buffer increases
solution density.)
Note 1: Sucrose or glycerol may be used instead of
Ficoll 400.
†
0.40 M
48.4 g
0.20 M
11.4 ml
0.01 M
20.0 ml
to 1000.0 ml
0.5 M
18.6 g
to 70.0 ml
~5.0 ml
to 100.0 ml
†
,10 ml)
to 7.0 ml
2.5 g
25.0 mg
to 10.0 ml
†
•
•
p15
p15
p15
p15
p15
•
•
•
•
•
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