Read Types - Molecular Devices SpectraMax ABS User Manual

Read Types

The instrument supports the following read types:
Endpoint Read Type
In an endpoint read type, a read of each plate well is taken in the center of each well, at a
single wavelength or at multiple wavelengths. Raw data values are reported as optical density
(OD) or % transmittance (%T).
Kinetic Read Type
In a kinetic read type, the instrument collects data over time with multiple readings taken in the
center of each well at regular intervals. To achieve the shortest possible interval for kinetic
reads, choose wavelengths in ascending order.
The software can do the following calculations based on raw data: VMax, VMax per Sec, Time
to VMax, and Onset Time. Kinetic reads can be single wavelength or multiple wavelength
readings.
The kinetic read type can collect data points in time intervals of seconds, minutes, or hours (up
to 99 hours).
Kinetic analysis has many advantages to determine the relative activity of an enzyme in
different types of plate assays, including ELISAs and the purification and characterization of
enzymes and enzyme conjugates. Kinetic analysis is capable of providing improved dynamic
range, precision, and sensitivity relative to endpoint analysis.
Spectrum Read Type
Spectrum reads are made using the scanning monochromators of the instrument and can
measure across the spectrum of wavelengths:
SpectraMax ABS - Wavelengths from 340 nm to 850 nm.
SpectraMax ABS Plus - Wavelengths from 190 nm to 1000 nm
A spectrum read measures optical density (OD) or %Transmittance (%T) across a spectrum of
wavelengths.
5065390 C
Chapter 4: Absorbance Read Mode
21