Cuvette Chamber - Molecular Devices SpectraMax ABS User Manual

Cuvette Chamber

The SpectraMax ABS Plus has a cuvette chamber on the top center of the instrument. Press
down on the cuvette chamber lid to open the lid and insert or remove a cuvette. The chamber
contains springs that position the cuvette in the proper alignment for a read. You must manually
close the cuvette door before you initiate a read.
Handle cuvettes on the frosted sides only. Place the cuvette into the chamber so that the
"reading" (clear) sides face the front and the rear of the instrument.
The guidelines for cuvette use in the instrument are the same as those that apply to any high-
quality spectrophotometer. You must ensure that the meniscus is comfortably above the light
beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly
with the beam. The light beam is 0.625 in (15.87 mm) above the cuvette bottom.
The instrument can accommodate standard-height (45 mm) and other cuvettes up to 56 mm in
height. The instrument does not accept short (25 mm high) microcuvettes. See
page 43.
Not all manufacturers' cuvettes are the same with regard to design, materials, or configuration.
Temperature uniformity within the cuvette can vary depending on the type of cuvette you use.
5065390 C
Chapter 1: Introduction
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