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Molecular Devices Digidata 1550B User Manual

Low-noise data acquisition system plus humsilencer adaptive noise cancellation system
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Digidata® 1550B
Low-Noise Data Acquisition System Plus HumSilencer™ Adaptive Noise
Cancellation System
User Guide
5040237 B
January 2021

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Summary of Contents for Molecular Devices Digidata 1550B

  • Page 1 Digidata® 1550B Low-Noise Data Acquisition System Plus HumSilencer™ Adaptive Noise Cancellation System User Guide 5040237 B January 2021...
  • Page 2 For research use only. Not for use in diagnostic procedures. The trademarks mentioned herein are the property of Molecular Devices, LLC or their respective owners. These trademarks may not be used in any type of promotion or advertising without the prior written permission of Molecular Devices, LLC.

  • Page 3: Safety Information

    Read and observe all warnings, cautions, and instructions. Remember, the most important key to safety is to operate the instrument with care. WARNING! If the instrument is used in a manner not specified by Molecular Devices, the protection provided by the equipment might be impaired. Symbols on Instrument Labels Each safety label found on the instrument contains an alert symbol that indicates the type of potential safety hazard related to the label.
  • Page 4 For products under the requirement of the WEEE directive, contact your dealer or local Molecular Devices office for the procedures to facilitate the proper collection, treatment, recovery, recycling, and safe disposal of the device. 5040237 B...

  • Page 5: Table Of Contents

    Chapter 2: Installation Uninstalling Software Installing AxoScope Software or pCLAMP Software Suite (pCLAMP Only) Installing the Security Key Installing the Digidata 1550B Digitizer Configuring Software for Digidata 1550B Digitizer Use Chapter 3: Interface Description Front Panel Rear Panel Chapter 4: Maintenance...
  • Page 6 Digidata 1550B Low-Noise Data Acquisition System User Start Guide Digital Outputs Telegraph Inputs HumSilencer Adaptive Noise Cancellation Module AC Power Appendix A: Electromagnetic Compatibility 5040237 B...

  • Page 7: Chapter 1: Introduction

    Hz line-frequency noise and associated high-frequency harmonics. The Digidata 1550B digitizer communicates with the host computer using USB 2.0. The Digidata 1550B digitizer is a plug‐ and‐ play device, so it is automatically recognized by Windows. The Digidata 1550B digitizer is supported on Windows systems by AxoScope software Version 11 or newer and by pCLAMP Clampex software Version 11.

  • Page 8: Programming

    50 Hz or 60 Hz. This AC line-voltage is sampled by circuitry inside the Digidata 1550B digitizer. The start of every line power‐ cycle is reported to the high-speed logic section of the processor. Using this timing signal as an input to mark the beginning and end of the power line-cycle, the digitization circuitry inside the Digidata 1550B digitizer averages the non-DC signal input to the ANC-enabled channels over 50 cycles.
  • Page 9 Chapter 1: Introduction The HumSilencer system is designed to adapt quickly to changes in noise. In a country with a 60 Hz electrical grid, the response time is about 5/6th of a second to adapt to changes in the line noise. In a country with a 50 Hz electrical grid, the response time is about 1 second. However large fast transients are problematic as a result of this ability to adapt quickly to changes in noise.
  • Page 10 Digidata 1550B Low-Noise Data Acquisition System User Start Guide 5040237 B...

  • Page 11: Chapter 2: Installation

    To uninstall the software: 1. Go to Windows Start > All Programs >Molecular Devices (or Axon Laboratory). 2. Open the folder for the software version to be uninstalled, such as pCLAMP 10.6.

  • Page 12: Installing Axoscope Software Or Pclamp Software Suite

    Clampex software are invalid. Installing the Digidata 1550B Digitizer 1. Connect the power cord to the wall, and then to the Digidata 1550B digitizer rear panel AC POWER input connector. 2. Attach the USB 2.0 cable to a USB 2 or USB 3 port on your computer, and to the digitizer.

  • Page 13: Configuring Software For Digidata 1550B Digitizer Use

    1. Run AxoScope software or Clampex software by double-clicking on the icon on the Windows desktop. 2. Click Configure > Digitizer to open the Digitizer dialog, then click Change (). 3. Select Digidata 1550B Series from the Digitizer Type list. 5040237 B...
  • Page 14 6. Click OK to exit the Digitizer dialog. The front panel yellow READY light is continuously on only when the software connects to the digitizer. After warming up (allow one hour), the Digidata 1550B digitizer is ready for experiments. 5040237 B...

  • Page 15: Chapter 3: Interface Description

    Inputs are connected to the Analog ground. All of these channels can be used simultaneously without any reduction in throughput for each channel. These input channels are typically used to digitize biological signals. Depending on the Digidata 1550B digitizer model, when the HumSilencer Adaptive Noise Cancellation System is available, Analog Input Channels #0, or Analog Input Channels #0, #2, #4, and #6 are configurable for eliminating ground signal noise.
  • Page 16 The HumSilencer Adaptive Noise Cancellation System (ANC) is integrated into Analog Input Channels #0, #2, #4, and #6 of the Digidata 1550B digitizer, depending on the model, so no additional hardware installation is required. When it is in use, it eliminates electrical hum interference at 50 Hz or 60 Hz and the associated high‐...
  • Page 17 Chapter 3: Interface Description When AxoScope software or Clampex software starts, HumSilencer > Adapt check box on the Real Time Controls panel is automatically enabled and learning the line-frequency noise (Figure 3-3). Figure 3-3: The HumSilencer system Real Time Controls panel items in active learning mode, but not actively canceling noise The Real Time Controls panel items for the HumSilencer system include (Figure...

  • Page 18: Digital Outputs

    Software v11.2 User Guide . Digital Outputs Digital Outputs 0–7 are on the front panel of the Digidata 1550B digitizer. These output levels can be set to high (+5 V) or low (0 V) TTL-level compatible states. They can be used to trigger a wide variety of external devices.

  • Page 19: Rear Panel

    Chapter 3: Interface Description Rear Panel There are several connectors on the rear panel of the Digidata 1550B digitizer: four BNCs, one USB connector, one 25-pin connector, and one AC POWER input. Figure 3-5: Rear Panel of the Digidata 1550B digitizer...
  • Page 20 Digidata 1550B Low-Noise Data Acquisition System User Start Guide 5040237 B...

  • Page 21: Chapter 4: Maintenance

    Test HumSilencer Adaptive Noise Cancellation System Functionality on page 26 Configure With the Software If you are able to configure the Digidata 1550B in the pCLAMP software, then the digitizer is properly installed in Windows. Continue to the next test.
  • Page 22 Digidata 1550B Low-Noise Data Acquisition System User Start Guide 3. In the Change Digitizer dialog, select Digidata 1550B Series from the Digitizer Type list. 4. Click Scan to detect the digitizer. The first detected digitizer is assigned 0 and listed as Available. The Configuration information changes from Not present to reporting the selected digitizer model number, serial number, firmware version, channel availability (0, 1, or 4), and the OK button enables.
  • Page 23 Chapter 4: Maintenance Verify Computer USB Port To verify that you have a USB 2 or USB 3 port in your computer: 1. In Windows 10, right‐ click This PC and select Properties. 2. In the System > Control Panel dialog, click Device Manager. 3.
  • Page 24 Digidata 1550B Low-Noise Data Acquisition System User Start Guide 2. Set up a protocol through the Acquire > New Protocol menu command, and then on the Mode/Rate tab, select the Gap‐ free mode. On the Outputs tab, for each of the Analog OUT Channels, select the matching signal (for example, OUT 1) from its list.
  • Page 25 Chapter 4: Maintenance Test Input Signal Functionality To test analog inputs signals are working with either AxoScope software or Clampex software: 1. Click Lab Bench (or click Configure > Lab Bench) and select the Output Signals tab. For each of the Digitizer Channels (for example, Analog OUT #0), select a matching Signal (for example, OUT 0), and configure that signal with unity scaling (for example, Scale factor (V/V): 1).
  • Page 26 Digidata 1550B Low-Noise Data Acquisition System User Start Guide Test HumSilencer Adaptive Noise Cancellation System Functionality Not all Digidata 1550B include the HumSilencer Adaptive Noise Cancellation System (ANC). A sticker on the back of your digitizer specifies if the ANC system is installed and the number of...

  • Page 27: Grounding And Minimizing Noise

    To avoid ground-loops, Molecular Devices recommends that you plug in the Digidata 1550B digitizer to the same power strip as the amplifier. Also, be aware that each Analog Input BNC on the Digidata 1550B digitizer is a single-ended input (all BNC shells are connected to signal ground).

  • Page 28: Replacing Fuses

    If the checks fail to fix your power loss, replace the fuse. If you need replacement fuses, contact Molecular Devices Technical Support. The Digidata 1550B digitizer uses a pair of fuses located in a fuse carrier under the AC input port...
  • Page 29 7. Reconnect the power cord to the AC input port. 8. Switch on the power from the front of the instrument. Note: If the instrument still does not power on after changing the fuses, contact Molecular Devices Technical Support. 5040237 B...
  • Page 30 Digidata 1550B Low-Noise Data Acquisition System User Start Guide 5040237 B...

  • Page 31: Chapter 5: Troubleshooting

    Ready Light is Flashing When the Ready light on the front panel is flashing, the Digidata 1550B digitizer is in a major fault state. Contact Molecular Devices Technical Support.

  • Page 32: Problems With Analog Or Digital Intputs

    Reset the digitizer by turning it off and then back on. Then restart the computer. For Windows, reset the Windows registry digitizer settings back to the manufacturer defaults ( for example, Start > All Programs > Molecular Devices > pCLAMP 11 > Reset to Program Defaults).

  • Page 33: Problems With Data Throughput

    In Configure > Program Options, select Disable screen saver during data acquisition. Obtaining Support Molecular Devices is a leading worldwide manufacturer and distributor of analytical instrumentation, software, and reagents. We are committed to the quality of our products and to fully supporting our customers with the highest level of technical service.
  • Page 34 Digidata 1550B Low-Noise Data Acquisition System User Start Guide Clampex software users: 1. Connect Analog Out 0 to Analog In 0. 2. From Acquire > New Protocol, run an Episodic stimulation mode protocol with a default waveform specified. 3. Click the View Only button. Do you see the default waveform?

  • Page 35: Chapter 6: Specifications

    Chapter 6: Specifications This section lists instrument specifications, electrical requirements, instrument dimensions, and space requirements for the Digidata 1550B digitizer. Operational and Environmental Table 6-1: Operational and Environmental Specifications Item Description Weight 3.6 kg Dimensions (H x W x D)

  • Page 36: Analog Inputs

    Digidata 1550B Low-Noise Data Acquisition System User Start Guide Analog Inputs Table 6-2: Analog Inputs Specifications Item Description Number of channels Type of channels single‐ ended Resolution 16‐ bit, 1 in 65536 Sample rates per channel 1 Hz to 500 kHz ‐...
  • Page 37 Chapter 6: Specifications Digital Outputs Table 6-5: Digital Outputs Specifications Item Description Number of bits 16 (8 supported in software) Output driver Advanced CMOS (AC) compatible Output current ±4 mA SCOPE trigger Shared bit on DO #15 A DB 25‐ pin female connector is provided on the rear panel with the following pin assignments as listed in Table 6-6.
  • Page 38 Digidata 1550B Low-Noise Data Acquisition System User Start Guide Table 6-6: Pin Assignments Specifications (continued) Pin Number Digital Output Internal Use (or 13) Scope Output (or 15) Analog ground 1. Pin 24 is configured as the front panel SCOPE output, using the hardware’s 16th digital bit (15).

  • Page 39: Appendix A: Electromagnetic Compatibility

    Appendix A: Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Cet appareil ISM est confomre à la norme NMB-001 du Canada. ISM Equipment Classification (Group 1, Class A) This equipment is designated as scientific equipment for laboratory use that intentionally generate and/or use conductively coupled radio-frequency energy for internal functioning, and are suitable for use in all establishments, other than domestic and those directly connected to a low voltage power supply network which supply buildings used for domestic...
  • Page 40 The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. ©2021 Molecular Devices, LLC. Specifications subject to change without notice. Patents: www.moleculardevices.com/patents All rights reserved. FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES...

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