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Summary of Contents for Molecular Devices SpectraMax ABS
- Page 1 SpectraMax ABS SpectraMax ABS Plus Microplate Spectrophotometers User Guide 5065390 C January 2021...
- Page 2 For research use only. Not for use in diagnostic procedures. The trademarks mentioned herein are the property of Molecular Devices, LLC or their respective owners. These trademarks may not be used in any type of promotion or advertising without the prior written permission of Molecular Devices, LLC.
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Page 3: Table Of Contents
Read Types Chapter 5: Maintenance Cleaning the Instrument Replacing Fuses Before You Move the Instrument Chapter 6: Troubleshooting Opening the Drawer Manually Obtaining Support Chapter A: Instrument Specifications SpectraMax ABS Specifications SpectraMax ABS Plus Specifications Electromagnetic Compatibility Appendix B: Accessories 5065390 C... -
Page 4: Safety Information
Follow the related safety information. The following user attention statements might be displayed in the text of Molecular Devices user documentation. Each statement implies the amount of observation or recommended procedure. - Page 5 For products under the requirement of the WEEE directive, contact your dealer or local Molecular Devices office for the procedures to facilitate the proper collection, treatment, recovery, recycling, and safe disposal of the device.
- Page 6 The instrument contains moving parts that can cause injury. Under normal conditions, the instrument is designed to protect you from these moving parts. WARNING! If the instrument is used in a manner not specified by Molecular Devices, the protection provided by the equipment might be impaired. To prevent injury: Never try to exchange labware, reagents, or tools while the instrument is operating.
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Page 7: Chapter 1: Introduction
ELISA, nucleic acid, protein, enzymatic type homogeneous and heterogeneous assays, microbial growth, endotoxin testing, and pipettor calibration. The SpectraMax ABS supports the Visible Absorbance (ABS) read mode while the SpectraMax ABS Plus supports the UV and Visible Absorbance (ABS) read mode with the following read types: Endpoint: At a single point in time. -
Page 8: Computer Integration
SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. -
Page 9: Plate Controls
Opening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. - Page 10 SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Note: Use high quality support frames or holders when you use strip-wells. You should fill the frame or holder with empty strips to avoid warping. Shake To mix of the contents of the wells, you can define settings to shake the plate linearly along the long axis at preset intervals.
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Page 11: Cuvette Chamber
Chapter 1: Introduction Cuvette Chamber The SpectraMax ABS Plus has a cuvette chamber on the top center of the instrument. Press down on the cuvette chamber lid to open the lid and insert or remove a cuvette. The chamber contains springs that position the cuvette in the proper alignment for a read. You must manually close the cuvette door before you initiate a read. -
Page 12: Temperature Regulation
SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Temperature Regulation When you power on the instrument the incubator is off. The temperature in the plate chamber is ambient and isothermal. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C. -
Page 13: Chapter 2: Setting Up The Instrument
Chapter 2: Setting Up the Instrument Before you unpack and set up the instrument, prepare a dry, flat work area that has sufficient space for the instrument, host computer, and required cables. To provide access for disconnecting power from the instrument, maintain a 20 cm to 30 cm (7.9 in. to 11.8 in.) gap between the rear of the instrument and the wall. -
Page 14: Connecting Instrument Cables
4. Turn the instrument around so that the front of the instrument now faces you. Note: Ensure no cables run beneath the instrument. 5. Remove the tape from the cuvette door on the SpectraMax ABS Plus. 6. Power on the instrument and wait for the plate drawer to open. -
Page 15: Chapter 3: Getting Started
Chapter 3: Getting Started Now that you have installed the SoftMax Pro Software on the computer, removed the tape from the drawer and the cuvette port, and connected the cables, it is time to get started. WARNING! Never use the instrument in an environment where potentially damaging liquids or gases are present. - Page 16 SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide 5065390 C...
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Page 17: Chapter 4: Absorbance Read Mode
Chapter 4: Absorbance Read Mode The instrument uses the Absorbance (ABS) read mode to measure the Optical Density (OD) of the sample solutions. Absorbance is the quantity of light absorbed by a solution. To measure absorbance accurately, it is necessary to eliminate light scatter. If there is no turbidity, then absorbance = optical density. -
Page 18: Pathcheck Technology
1 cm cuvette. The instrument uses the factory installed water constant to obtain the 1 cm values. For the SpectraMax ABS Plus you can read a cuvette that contains deionized water or buffer to use the Cuvette Reference correction method (typically not necessary when you use aqueous solutions with minimal alcohol, salt, or organic solvent content). - Page 19 Chapter 4: Absorbance Read Mode Note: You must select the PathCheck check box before a read because you cannot apply the PathCheck technology after the read. After you read a plate with PathCheck technology turned on, the software stores PathCheck information permanently within the document.
- Page 20 SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Using a Plate Background OD If your sample volumes are not identical or if you choose not to use a Plate Blank, then you must use a Plate Background OD. Omitting a Plate Background OD results in artificially high values after being normalized by the PathCheck technology.
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Page 21: Read Types
Spectrum reads are made using the scanning monochromators of the instrument and can measure across the spectrum of wavelengths: SpectraMax ABS - Wavelengths from 340 nm to 850 nm. SpectraMax ABS Plus - Wavelengths from 190 nm to 1000 nm A spectrum read measures optical density (OD) or %Transmittance (%T) across a spectrum of wavelengths. - Page 22 SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide 5065390 C...
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Page 23: Chapter 5: Maintenance
Chapter 5: Maintenance Perform only the maintenance tasks described in this guide. Contact a Molecular Devices service engineer to inspect and perform a preventive maintenance service on the instrument each year. See Obtaining Support on page Before you operate the instrument or perform maintenance operations, make sure you are familiar with the safety information in this guide. -
Page 24: Replacing Fuses
SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Always turn the power off and disconnect the power cord from the main power source before you use liquids to clean the instrument. Periodically clean the outside surfaces of the instrument using a cloth or sponge that has been lightly dampened with water. -
Page 25: Before You Move The Instrument
8. Turn on power to the instrument. Note: If the instrument still does not power on after you change the fuses, contact Molecular Devices technical support. Before You Move the Instrument Before you move the instrument, make sure that the new location is a dry, flat work area that has sufficient space for the instrument, host computer, and required cables. - Page 26 SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide 5065390 C...
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Page 27: Chapter 6: Troubleshooting
If the fatal error message continues to display on power up, record the error message number and contact Molecular Devices Technical Support or your local representative for assistance. If the instrument functions normally when you use the SoftMax Pro Software, no errors should be in the buffer (except error number 100). - Page 28 SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Table 2: Error Codes 100-199: Unrecognized Command Errors Sent From the Computer Error Error Message Notes Code command not Command string not recognized. found invalid argument Command argument not recognized.
- Page 29 Chapter 6: Troubleshooting Table 3: Error Codes 200-299: Firmware Errors Error Error Message Notes Code assert failed Firmware error. bad error number Firmware error. receive queue overflow Caused by external device sending too much data over serial port and ignoring flow control. serial port parity error Parity bit error detected with incoming serial data.
- Page 30 SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Table 4: Error Codes 300-399: Hardware Errors Error Error Message Notes Code thermistor faulty Unable to read a reasonable thermistor value. Thermistor faulty or disconnected, Main board problem, or ambient temperature out of range.
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Page 31: Opening The Drawer Manually
This action will not harm the instrument, but should only be taken if the first two options fail to open the drawer. If you are still unable to open the drawer, contact your local Molecular Devices representative. 5065390 C... -
Page 32: Obtaining Support
If you still need assistance after consulting the Knowledge Base, you can submit a request to Molecular Devices Technical Support. You can contact your local representative or Molecular Devices Technical Support at 800-635-5577 X 1815 (North America only) or +1 408-747-1700. In Europe call +44 ... -
Page 33: Chapter A: Instrument Specifications
100 µL and 300 µL. Technical specifications are subject to change without notice. Molecular Devices provides validation documentation for software and hardware, as well as absorbance, fluorescence, and ®... -
Page 34: Spectramax Abs Specifications
SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide SpectraMax ABS Specifications Technical specifications are subject to change without notice. Photometric Performance SpectraMax ABS Item Description Wavelength range 340 nm to 850 nm Wavelength selection Monochromator tunable in 1 nm increments ≤... - Page 35 Chapter A: Instrument Specifications Absorbance Mode Measurement Time for Plates (calibration off) SpectraMax ABS Item 96-Wells Endpoint standard read *Measurement 9 seconds single wavelength 19 seconds dual conditions: endpoint, column priority (for dual- wavelength 425 & 650 nm wavelength measurements), calibrate off.
- Page 36 SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Temperature Regulation SpectraMax ABS Item Description Read chamber Isothermal when temperature regulation is not enabled Range 5°C above ambient to 45°C when temperature regulation enabled. The ambient temperature must be >20°C to achieve temperature regulation at 45°C.
- Page 37 Chapter A: Instrument Specifications General Instrument SpectraMax ABS Item Description Self-diagnosis Continuous on-board diagnostics Computer interface USB cable Supported plates All 96-well and strip-well plates, including lids CAUTION! To prevent damage to the instrument, the height of the plate must not exceed 17 mm, including the lid if the plate is lidded.
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Page 38: Spectramax Abs Plus Specifications
SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide SpectraMax ABS Plus Specifications Technical specifications are subject to change without notice. Photometric Performance SpectraMax ABS Plus Item Description Wavelength range 190 nm to 1000 nm Wavelength selection Monochromator tunable in 1 nm increments ≤... - Page 39 Chapter A: Instrument Specifications Absorbance Mode Measurement Time for Plates (calibration off) SpectraMax ABS Plus Item 96-Wells 384-Wells Endpoint standard read 9 seconds single wavelength 19 seconds 29 seconds single *Measurement dual wavelength 425 & 650 nm wavelength 59 seconds conditions: endpoint, dual wavelength 425 &...
- Page 40 SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Temperature Regulation SpectraMax ABS Plus Item Description Read chamber Isothermal when temperature regulation is not enabled Range 5°C above ambient to 45°C when temperature regulation enabled. The ambient temperature must be >20°C to achieve temperature regulation at 45°C.
- Page 41 Chapter A: Instrument Specifications General Instrument SpectraMax ABS Plus Item Description Self-diagnosis Continuous on-board diagnostics Computer interface USB cable Supported plates All 96-well and strip-well plates, including lids CAUTION! To prevent damage to the instrument, the height of the plate must not exceed 17 mm, including the lid if the plate is lidded.
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Page 42: Electromagnetic Compatibility
SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Cet appareil ISM est confomre à la norme NMB-001 du Canada. ISM Equipment Classification (Group 1, Class A) -
Page 43: Appendix B: Accessories
Appendix B: Accessories Available Accessories Part Number Description 0200-6117 SpectraTest ABS1 Absorbance Validation Test Plate 9000-0161 Cuvette Absorbance Validation Kit R8024 SpectraPlate-Quartz UV-transparent Microplate 4400-0002 Power Cord (US, Canada, Japan, Mexico, India) 4400-0036 Power Cord, EC1 (Germany, France, Scandinavia, Italy, Korea) 4400-0037 Power Cord, EC2 (UK, Indonesia, Singapore, Malaysia) 4400-0038... - Page 44 SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Standard, Semi-micro, and Microcuvettes (Hellma) Item Standard Semi-Micro Micro Hellma Cat. No. 105.004 104.002 108.002 Internal 10 x 10 4 x 10 4 x 10 2 x 10 2 x 10...
- Page 45 The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. ©2021 Molecular Devices, LLC. Specifications subject to change without notice. Patents: www.moleculardevices.com/patents All rights reserved. FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES...
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