Chapter 1: Imagexpress Pico Automated Cell Imaging System - Molecular Devices ImageXpress Pico Calibration Kit Manual

Automated cell imaging system with cellreporterxpress software version 2.5
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Chapter 1: ImageXpress Pico Automated Cell Imaging
System
The Molecular Devices® ImageXpress® Pico Automated Cell Imaging System is an all-in-one
platform for automatically acquiring and analyzing images from fluorescently labeled
biological samples in plates and slides. It enables you to increase the throughput of your
image acquisition and analysis, allowing you to gain insights in minutes. With the addition of
a modular option, the system provides environmental control for live cell imaging.
The core hardware component of the imaging system is a custom-designed, fully automated,
epi-illumination fluorescence microscope. The rapid autofocus and precision sample
movement features of the microscope allow large numbers of high-resolution images to be
acquired in the shortest possible time. All key optical and mechanical elements are
motorized, which allows complete control of the instrument configuration.
When used in combination with the CellReporterXpress® Image Acquisition and Analysis
Software, which features powerful image analysis capabilities and more than 25 available
predefined experimental protocols, the ImageXpress Pico system becomes an extremely
flexible device, ideally suited for user-defined, automated assays.
Key components of the instrument include the following:
Built-in, internal light source comprised of six high-powered LEDs enables very high
sensitivity fluorescent imaging. Additional LED-based light sources allow transmitted light
and overview imaging.
High-sensitivity, 5-megapixel CMOS camera.
Hardware-based autofocus system with precision motorized Z-stage focus.
High-transmission fluorescence imaging optics with world-class chromatic aberration
correction, resolution, and image flatness.
Secondary camera provides overview imaging for slides.
Precision motorized sample (X-Y) stage.
High-quality Leica objectives in a six-position turret.
Filter cubes in a six-position turret.
Motorized selection of stage position, filter cubes, and objectives.
Temperature control up to 40°C (104° F) for live cell imaging.
Optional environmental control system, which enables you to regulate humidity, CO
and O
imaging.
Operation and configuration control by the integrated CellReporterXpress software.
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inside the environmental control cassette for multi-day, live-cell, time-lapse
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