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- Page 1 User Instructions Ultrasonic homogenisers Ultrasonic homogeniser HD 4100 Valid for: HD 4050 Volume: 0.5 ml - 100 ml HD 4100 Volume: 2 ml - 200 ml HD 4200 Volume: 5 ml - 1000 ml HD 4400 Volume: 100 ml - 3000 ml 51376-010 GB/2020-10...
- Page 2 Copyright & limit of liability This document may not be reproduced, either in full or in extracts, without prior approval of BANDELIN electronic GmbH & Co. KG, hereinafter referred to as BANDELIN. The German original is the binding version of this document. Any diff erences in the translation are not binding and have no legal eff...
- Page 3 Identifi es instructions that must be followed in the described Handling instructions sequence. 51376-010 GB/2020-10 3 / 46 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com...
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Page 4: Table Of Contents
General use ........................24 Selecting a suitable probe .................... 26 4.3.1 Mounting and dismounting of the probes ..............27 4 / 46 51376-010 GB/2020-10 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... - Page 5 Consumable materials ....................40 Taking the unit out of service ..................40 Key words ........................41 Informative appendices Tools for mounting/dismounting 51376-010 GB/2020-10 5 / 46 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com...
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Page 6: Product Description
Rear side of the ultrasonic generator: • Mains socket (10) with fuse holder • Remote control connector(11) • RS 232 interface (12) 6 / 46 51376-010 GB/2020-10 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... -
Page 7: Mode Of Operation
A declaration of conformity can be requested from the manufacturer by providing the serial number. 51376-010 GB/2020-10 7 / 46 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... -
Page 8: Technical Data
In the case of highly viscous media, this can also result in the desired amplitude not always being reached. depending on the connected ultrasonic converter 8 / 46 51376-010 GB/2020-10 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... -
Page 9: Technical Data, Ultrasonic Converters
EMC directions; information in this regard is found in chapter 1.4.4 e-procurement e-cl@ss: HD device classifi cation: 32-08-02-02 HD accessories classifi cation: 32-08-92-05 51376-010 GB/2020-10 9 / 46 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... -
Page 10: Electromagnetic Ambient Conditions (Emc)
With all other modes of operation, the input works as an edge-controlled changeover switch. The input resistance measures approx. 50 kΩ. 10 / 46 51376-010 GB/2020-10 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... - Page 11 The inputs and outputs of the remote control connector and RS-232 interface may only be connected with devices of protection class I that are connected to the same mains supply circuit. 51376-010 GB/2020-10 11 / 46 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com...
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Page 12: Warnings And Safety Precautions
• Only use the prescribed tool for mounting and dismounting (see appendix A). • Do not use any bent probes ( unstable operation, loss of power). 12 / 46 51376-010 GB/2020-10 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... -
Page 13: Preparation
Before startup, the ultrasonic homogeniser is to be left to stand at its operating location for 2 hours so that it may adapt to the ambient conditions. 51376-010 GB/2020-10 13 / 46 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... -
Page 14: Scope Of Delivery
Connect to a grounded socket only if type is suitable. 14 / 46 51376-010 GB/2020-10 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... -
Page 15: Start-Up
Ultrasound output blinking green: Remote control through the RS 232 interface red light: Error messages blinking red: Warning messages 51376-010 GB/2020-10 15 / 46 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... -
Page 16: Turning The Homogeniser On/Off
. If the parameter has number values that can be edited, the editing mode is then activated with the arrow key . In the marked area (inverse view) of the parameter, the desired value can be 16 / 46 51376-010 GB/2020-10 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... -
Page 17: Sonication Time [Time Set]
0.000 kJ Energy 0.000 kJ START STOP back to stand-by mode proceed to next set value or operating parameter 51376-010 GB/2020-10 17 / 46 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... -
Page 18: Pulsation [Pulsation]
The pulse period duration is the sum of the turn-on and turn-off time t = t 18 / 46 51376-010 GB/2020-10 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... -
Page 19: Temperature Monitoring [Temperature]
Pulsation 4.0s 4.0s Temperature 31°C No sensor: ---°C Energy 0.000 kJ START STOP back to stand-by mode 51376-010 GB/2020-10 19 / 46 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... -
Page 20: Miscellaneous Functions And Settings
The default value is 22. probe check scan frequency contrast operating mode Ampl. display Energy batch mode 03.01 - 2016-08-085 20 / 46 51376-010 GB/2020-10 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... -
Page 21: Loading / Saving Sonication Programs
Pulsation Temp alarm at 0° Temp control load []* store []* load []* store []* quit [start] quit [start] 51376-010 GB/2020-10 21 / 46 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... -
Page 22: Batch Operation (Batch Processing)
Note: In this operating mode no more changes of settings are possible within the stand-by mode or ultrasonic operation. 22 / 46 51376-010 GB/2020-10 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... -
Page 23: Activating / Deactivating The Ultrasound
• The displayed value for energy is just a reference value and does nocht correspond to the acoustic energy actually introduced. 51376-010 GB/2020-10 23 / 46 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... -
Page 24: Use
Set the immersion depth for the probe (approx. 10 -20 mm). If needed, connect the temperature sensor and position it in the medium. 24 / 46 51376-010 GB/2020-10 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... - Page 25 Clean the probe and check the wear on the sound emitting surface at regular intervals, see chapter 5.1. 51376-010 GB/2020-10 25 / 46 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com...
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Page 26: Selecting A Suitable Probe
After technical updating of the probes, the data may be changed. The stored data in the program are always valid! 26 / 46 51376-010 GB/2020-10 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... -
Page 27: Mounting And Dismounting Of The Probes
The tool required for mounting/ dismounting is included in the scope of supply. The mounting surfaces are the contact surfaces between the standard/booster horn and the probe. 51376-010 GB/2020-10 27 / 46 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... -
Page 28: Mounting And Dismounting The Titanium Fl At Tips
Sickle spanner HS 40/42 laying atop work surface. screw on TT 213 TT 213 unscrew Sickle spanner laying atop work surface. 28 / 46 51376-010 GB/2020-10 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... -
Page 29: Mounting And Dismounting Of Titanium Probes
With the UW 50 use sickle spanner HS 25/28 - the probe is mounted directly onto the UW 50. Dismount in the reverse order. Dismounting Assembly Sickle spanner HS 40/42 Spanner here: Spanner SW 10 51376-010 GB/2020-10 29 / 46 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... -
Page 30: Dismounting And Mounting The Standard/Booster Horns
One sickle spanner is part of the s cope of delivery of one standard/booster horn. Ultrasonic converter Standard/booster horn Vigorously turn sickle spanner HS 40/42 long, in the opposite direction 30 / 46 51376-010 GB/2020-10 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... - Page 31 The ultrasonic oscillating system and its electrical connections would be damaged as a result. 51376-010 GB/2020-10 31 / 46 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com...
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Page 32: Cleaning And Maintenance Of The Homogeniser
• Housing is to be wiped off only from the outside, use a suitable surface disinfectant if needed. Afterwards, allow to dry off or wipe dry. 32 / 46 51376-010 GB/2020-10 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... -
Page 33: Treatment Of Contaminated Parts In The Ultrasonic Converter, Vessels And Accessories Pertaining To The Medical Fi Eld
During extended periods without use, the homogeniser should be stored in a cool, dry place. The ultrasonic converter should be covered in order to protect the electronics from outside contamination. 51376-010 GB/2020-10 33 / 46 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... -
Page 34: Maintenance And Repair
01.02 - 2014-08-20 01.02 - 2014-08-20 probe check scan frequency contrast START operating mode Ampl. STOP batch mode 01.02 - 2014-08-20 34 / 46 51376-010 GB/2020-10 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... -
Page 35: Error Analysis
10 minutes, if necessary check with a homogenizer switches off temperature sensor that the operating temperature of the GM has dropped to < 50 °C. 51376-010 GB/2020-10 35 / 46 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... - Page 36 4.3.1. Wrong resonance frequency? Conduct frequency scan, see chapter 6.2 (search frequency). 36 / 46 51376-010 GB/2020-10 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com...
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Page 37: Repairs And Service
Fax: +49-(0)-30 – 76 88 02 00 13 In the case of returns, the general terms of delivery of BANDELIN electronic GmbH & Co. KG shall apply. In addition, the homogeniser must be cleaned and decontaminated (if necessary), see the following chapter. -
Page 38: Decontamination Certifi Cate
03.01 - 2016-08-085 The software version provided here is only an example, the actual specifi cations may diff er. 38 / 46 51376-010 GB/2020-10 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... -
Page 39: Accessories
The sonication of liquids generates noise. The sound proof box mutes the sound emission up to approximately 30 dB(AU) LS 40 51376-010 GB/2020-10 39 / 46 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... -
Page 40: Consumable Materials
• Metal accessories such as probes or standard horns should be decontaminated and disposed of as metal waste. • The packing is recyclable. 40 / 46 51376-010 GB/2020-10 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... -
Page 41: Key Words
Laboratory fi eld Liquid 7, 12, 13, 24, 39 Energy 7, 8, 16, 17, 23 Error message 15, 20, 34, 35 51376-010 GB/2020-10 41 / 46 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com... - Page 42 Protection class Suitable for continuous operation Pulsation time 8, 16 Surface (sound-emitting) 25, 32, 33, 34 Purpose 7, 9 42 / 46 51376-010 GB/2020-10 1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com...
- Page 43 8, 10, 17, 18, 19, Ultrasound output 15, 25 3, 13, 35 Viscosity 8, 23, 24 Voltage source 10, 12 BANDELIN electronic GmbH & Co. KG www.bandelin.com Zertifi ziert nach Heinrichstraße 3 – 4 info@bandelin.com ISO 9001 12207 Berlin +49 30 768 80-0...
- Page 45 Tools for mounting/dismounting Probes and standard/booster horns are highly-sensitive parts that must be mounted and dismounted with the greatest amount of care. For this reason, only use the following spanners for the mounting/dismounting of probes from standard/booster horns, as well as of standard/booster horns from the ultrasonic converter: Spanner Type Spanner MS 10, long...
- Page 46 Important: The Instructions for Use in this and other languages, as well as further information, can be found on the enclosed CD.
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