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How to use the Jeol 1010 TEM of GI (Liesbeth own GI version)
1.Load the specimen
Load a grid into the rod holder: USE ONLY THE TOP POSITION (blue arrow),
Specimen selection on 1 (The rear one is only a reserve one- Spec sel 2). Use the
white wing key with a feather tail (red arrow) to insert the slit over the cover: grab the
cover with the red dots oriented in the long axis, turn a quarter and gently open it.
Insert the grid specimen up. Close the cover and remove the wing key. (Check that all
covers are closed before inserting the rod in the goniometer. Be careful to not lose
the wing key and put it back in its hole on the standard.
If not already done, rotate the tilt of the insertion chamber (goniometer) to the zero
position (scale to be read in the scale on the left side) by releasing the lock
Version 2017-01-19 for GI use
h
(lift it
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Summary of Contents for JEOL 1010
- Page 1 How to use the Jeol 1010 TEM of GI (Liesbeth own GI version) 1.Load the specimen Load a grid into the rod holder: USE ONLY THE TOP POSITION (blue arrow), Specimen selection on 1 (The rear one is only a reserve one- Spec sel 2). Use the white wing key with a feather tail (red arrow) to insert the slit over the cover: grab the cover with the red dots oriented in the long axis, turn a quarter and gently open it.
- Page 2 slightly). When ready, close this lock (lift down to vertical position). Align the pin of the grid holder with the slot (line above pointing to the slot) in the goniometer. Gently press the grid rod straight into the opening. To make contact press the rod cylinder (metal color in ours) with a flat hand palm: a green light lights (line).
- Page 3 2. Viewing specimen, acquiring images right panel, left panel and central base of the column with xy navigation Version 2017-01-19 for GI use...
- Page 4 Press the HT knob under the plastic cover (it lights up) and check that the green light is ready. Beam current (3) rises to 040. Press the FIL knob on. Also here a green ready light goes up and the current rises from 40 until about 60µA.
- Page 5 Allow the grid to fall on a clean piece of paper and lift it with tweezers to set it back in the grid box. Close the cover and secure the wing key in its cavity. H ALIGNMENT JEOL 1010 TEM (GI) HOW TO CHECK THE SATURATION OF THE TEM FILAMENT?
- Page 6 Remove the upper and the lower condenser diaphragms by switching the level c to the right Go up and down with brightness (12). Adjust over and underfocus. If the A well centered filament gives a centered beam position swings, adjust the centering with the screws shift 10 and shift 13 Version 2017-01-19 for GI use...
- Page 7 If the filament (hairpin) becomes visible, VERY SLIGHTLY adjust the filament knob (under the brownish cover on the right panel) by turning it in clockwise direction until the pin shadow disappears. Close the brownish lid Spread the beam with brightness Reinsert the upper diaphragm c and the lower diaphragm.
- Page 8 Turn screw a counter-clockwise until the other edge is found. Do this while counting the number of turns, to know where the center is. Use screw b (first completely in until stop. Be careful not to … to prevent the diaphragm to fall into the column ??? FRESNEL FRINGES ADJUSTMENT Insert a carbon test grid (ASK Geert-Jan).
- Page 9 If Fresnel fringes appear in the holes of the carbon grid as lateral shadows, instead of a well-centered rings, adjust the center with screw xx and yy Explanations-Theory http://www.uiowa.edu/~cmrf/methodology/tem/tem_pg3.html To optimize imaging in the TEM a beam alignment should be performed prior to use. A tool for this alignment is a holey grid.
- Page 10 The final image is viewed by projection onto a phosphorescent screen which gives off photons when irradiated by the electron beam. A film camera is located beneath the phosphorescent screen. The screen is raised in order to expose a special photographic film with a thicker emulsion layer than light film.
- Page 11 One would not normally expect chromatic aberration to be a problem in an electron microscope, but, electromagnetic radiation of different energies converge at different focal planes. This is essentially the same problem as the chromatic aberration observed in the light microscope. To correct for chromatic aberration, increase accelerating voltage, improve the vacuum and/or use a thinner specimen.
- Page 12 Although diffraction can be useful, diffraction of electron waves around the aperture openings can interfere with the initial wave front. The results are an unclear or out-of- focused image. It is important to create a balance between reduction of spherical aberration and diffraction by selecting an appropriate sized aperture.
- Page 13 To correct for astigmatism, still using the holy grid, search for a small hole at high magnification (e.g. 100000x). Apply underfocus: Fresnel rings appear (see arrows here above). Apply just enough defocus so that the rim appears only just a little bit in one axis. ...
- Page 14 If the manual switch camera has been touched use one acquire round to reopen the camera. There are in principle two positions for two single grids on the holder. Each position can be found by switching SPEC SEL (left – bottom of the column). However, as a routine, only the position (1) is used.
- Page 15 - HT and FIL on again Bugs Version 2017-01-19 for GI use...
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