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Techne Touchgene Gradient Operator's Manual

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CONTENTS
SAFETY AND INSTALLATION
T o u c h g e n e
Touchgene Gradient Operator's Manual
G r a d i e n t
O P E R A T O R ' S
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M A N U A L
PAGE 1

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  • Page 1: Table Of Contents

    T o u c h g e n e G r a d i e n t O P E R A T O R ’ S M A N U A L CONTENTS Touchgene Gradient Operator’s Manual page SAFETY AND INSTALLATION Declaration of conformity English Français...
  • Page 2 T o u c h g e n e G r a d i e n t O P E R A T O R ’ S M A N U A L PAGE 2...

  • Page 3: Declaration Of Conformity

    Duxford Cambridge CB2 4PZ Declaration of Conformity Techne Unit Touchgene Gradient has been designed to comply with the following European Standards: EN 61326:1998 Electrical equipment for measurement, control and laboratory use. EMC requirements. EN 61010-1:1993 Safety requirements for electrical equipment for measurement, control and laboratory use.

  • Page 4: English

    Installation contact your supplier. See the De-contamination Certificate supplied with your unit. 1. All Techne units are supplied with a power cable. This may be integral or plug-in. 1. Cleaning 2. Before connecting the mains supply, check the voltage Before cleaning your unit ALWAYS disconnect from the against the rating plate.

  • Page 5: Français

    EXPOSE A DES TENSIONS QUI PEUVENT ETRE ne serait alors plus garantie. MORTELLES. Tous les appareils Techne sont conçus pour répondre aux normes CET APPAREIL NE CONTIENT AUCUN ELEMENT QUI de sécurité internationales et sont dotés d’un coupe-circuit en cas DEMANDE UN ENTRETIEN DE LA PART DE d’excès de température.

  • Page 6: Deutsch

    Sicherheit des Anwenders Nichts in dieser Gebrauchsanleitung darf als Garantie, Bedingung oder Voraussetzung verstanden werden, sei es die Beschreibung, Alle Benutzer von Techne Geräten müssen Zugang zu der Marktgängigkeit, Zweckdienlichkeit oder sonstiges bezüglich der entsprechenden Literatur haben, um ihre Sicherheit zu gewähren.

  • Page 7: Español

    Este manual ha sido preparado exclusivamente para los clientes Todos los usuarios de equipos Techne deben disponer de la de Techne (Cambridge) Limited y nada de lo especificado en este información necesaria para asegurar su seguridad. folleto de instrucciones se tomará como una garantía, condición o De acuerdo con las instrucciones contenidas en este manual y con aseveración de la descripción, comerciabilidad o adecuación para...

  • Page 8: The Touchgene Gradient

    THE TOUCHGENE GRADIENT Before using the Touchgene Gradient make sure you have read this manual carefully. If there is any doubt relating to the proper use of this equipment, the staff at Techne or your supplier will be pleased to assist you.

  • Page 9: Typical Specification At An Ambient Of 20°C

    G r a d i e n t O P E R A T O R ’ S M A N U A L Typical Specification at an ambient of 20°C For a detailed specification please contact Techne Sales Temperature Gradient Range 20°C to 70°C Maximum gradient 30°C...

  • Page 10: Working Conditions

    The Touchgene Gradient has many scientific laboratory applications, including DNA sequencing and PCR. Aspects of the PCR process are claimed in U.S. Patent Nos. 4,683,195, 4,683,202, and 4,965,188. Use of the Touchgene Gradient in such processes does not convey a licence to practice the processes themselves.

  • Page 11: Cleaning Your Touchgene Gradient

    You can clean the case or the screen of the Touchgene Gradient with a cloth dipped in water or ethanol (methanol or formaldehyde can also be used). No part of the case or cover should be immersed in the solvents.

  • Page 12: The Heated Lid

    M A N U A L The Heated Lid The heated lid is an integral part of the Touchgene Gradient. It can be switched “On” or “Off”, see ‘Program Defaults’ or ‘Editing screen’. To open the lid lift the back of the handle as shown and use it to lift the lid. To close the lid lower the lid onto the block with the samples in and press the handle shut.

  • Page 13: The Memory Card

    Either via copying software from a new unit and transferring it to an older unit, or by obtaining a copy of the new software from Techne This is a relatively easy process, see the “System Administration” card, and means that you can keep your unit up to date with the latest software.

  • Page 14: Before Switching On

    T o u c h g e n e G r a d i e n t O P E R A T O R ’ S M A N U A L Before Switching On Ensure that the voltage selector switch is set to the appropriate voltage for your supply.

  • Page 15: Power Up Screens

    M A N U A L POWER UP SCREENS The screen is a touch sensitive LCD graphics screen. Operator Techne input temperatures are Touchgene Gradient temperatures which you require the sample to achieve. Displayed temperatures are Serial number GPxxxx/xx either the temperatures Software version v.vv...

  • Page 16: System User Main Menu

    System User Main Menu Programs By touching this button you Techne can access all the programs Touchgene Gradient stored either internally or in the memory module, see page 18. Program defaults By touching this button you can set up the program defaults for a number of programs.

  • Page 17: Numerical Input Screen

    T o u c h g e n e G r a d i e n t O P E R A T O R ’ S M A N U A L Set program Defaults Once you have set the defaults to those required, you return to Initial denaturation 05m00s 94.0°c...

  • Page 18: Programs

    T o u c h g e n e G r a d i e n t O P E R A T O R ’ S M A N U A L PROGRAMS Touch the ‘Programs’ button in “System User Main Menu” Select program and you will go into the “Select xxxxxx bytes of memory free...

  • Page 19: Template Program Screens

    T o u c h g e n e G r a d i e n t O P E R A T O R ’ S M A N U A L Template Program Screens Any template program can be Program 2 STEP PCR TEMPLATE copied and given a new name.
  • Page 20 T o u c h g e n e G r a d i e n t O P E R A T O R ’ S M A N U A L Program 3 STEP PCR TEMPLATE Hot Start Disabled Number Cycles °...

  • Page 21: Existing Program Screen

    T o u c h g e n e G r a d i e n t O P E R A T O R ’ S M A N U A L Existing Program Screen If you select an existing Program Bill program from the “Programs”...

  • Page 22: Editing Programs

    T o u c h g e n e G r a d i e n t O P E R A T O R ’ S M A N U A L Editing Programs Program Bill Last Modified 19/08/2000 14:16:43 Heated Lid Disabled Heated Lid before program...
  • Page 23 T o u c h g e n e G r a d i e n t O P E R A T O R ’ S M A N U A L Touch any field you need to edit Editing Bill and it will become highlighted.
  • Page 24 T o u c h g e n e G r a d i e n t O P E R A T O R ’ S M A N U A L Editing Bill Initial denaturation Disabled ° Hot Start 05m00s 72.0 Deleting a Segment...
  • Page 25 T o u c h g e n e G r a d i e n t O P E R A T O R ’ S M A N U A L Segment Edit program function Touch ‘Segment’ to insert a Next Step segment.

  • Page 26: The Gradient

    T o u c h g e n e G r a d i e n t O P E R A T O R ’ S M A N U A L The Gradient The Set Temperature is the temperature that you require of the MIDDLE ROWS of samples in the block. The GRADIENT is the variation of the two extremes of the samples in the block;...
  • Page 27 T o u c h g e n e G r a d i e n t O P E R A T O R ’ S M A N U A L If you want to add other Edit program function information, touch ‘Continue’...

  • Page 28: Increment Or Decrement Temperature Or Time

    T o u c h g e n e G r a d i e n t O P E R A T O R ’ S M A N U A L Increment or decrement temperature or time. Under normal circumstances, the Hold Temperature of all segments is constant. However, it is possible to automatically increment or decrement the temperature of a specified segment of a programme.

  • Page 29: Save' Before 'Run

    T o u c h g e n e G r a d i e n t O P E R A T O R ’ S M A N U A L Edit program function Next Step Completion Touch ‘OK’ when you have ←...

  • Page 30: Running A Program

    T o u c h g e n e G r a d i e n t O P E R A T O R ’ S M A N U A L RUNNING A PROGRAM From the “Select program” Program “Your own”...

  • Page 31: While Running Programs

    T o u c h g e n e G r a d i e n t O P E R A T O R ’ S M A N U A L While Running Programs As the program progresses you will eventually get a screen similar to this diagram. The trace is plotted from the right-hand side and so initially you will have a blank graph with two axis lines.

  • Page 32: Data Logs

    Exit Printing Data Logs As the Touchgene Gradient runs, profile information is stored on the Memory Card. This information defines the times and temperatures at various points during the run. A range of events creates data which is used to create a profile of the run.
  • Page 33 T o u c h g e n e G r a d i e n t O P E R A T O R ’ S M A N U A L A printed profile is 80 columns wide and uses only standard characters in the ASCII range 1 to 127: thus all common dot matrix, pin fed, continuous stationery printers can be used.

  • Page 34: Using Gensoft

    M A N U A L Using Gensoft The “Gensoft” software package is available to enable you to connect the Touchgene Gradient to a computer via a ‘Comms’ port. “Gensoft” offers: A Daisy chaining a number of units together to a maximum of 32 units B Creation of thermal cycling protocols through as easy to use Microsoft Windows user interface and transmitting them to one or more units.

  • Page 35: Additional Information

    Please include details of the fault observed and remember to return the unit in its original packing. Techne accept no responsibility for damage to units which are not properly packed for shipping: if in doubt, contact your supplier, giving the full serial number of the unit and software version number (shown when the unit is first switched on).

  • Page 36: Interchangeable Blocks

    T o u c h g e n e G r a d i e n t O P E R A T O R ’ S M A N U A L Interchangeable Blocks Block removal Ensure that the unit is turned off and disconnected from mains.

  • Page 37: Accessories

    Flat plate block for in situ (non gradient) For full details of “Gensoft” contact your dealer or Techne Sales Department. Replacement parts The following replacement parts can be obtained from Techne or your Techne dealer: Item Nº Description Quantity required...
  • Page 38 T o u c h g e n e G r a d i e n t O P E R A T O R ’ S M A N U A L PAGE 38...
  • Page 39 T o u c h g e n e G r a d i e n t O P E R A T O R ’ S M A N U A L PAGE 39...
  • Page 41 OPERATOR’S MANUAL Issue F. Date of issue: 26th September 2000  Touchgene is the registered trademark of Techne Incorporated, Princeton, NJ, USA.  The Techne logo is the trademark of Techne (Cambridge) Limited and Techne Inc. © Techne (Cambridge) Limited, 2000...

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