Techne TC-PLUS Operator's Manual
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Techne TC-PLUS Operator's Manual

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TC-PLUS Satellite and
Techne Workbench PC Software
OPERATOR'S MANUAL
Version 1.3
11/10

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  • Page 1 TC-PLUS Satellite and Techne Workbench PC Software OPERATOR’S MANUAL Version 1.3 11/10...

  • Page 3: Table Of Contents

    INTRODUCTION BEFORE USE Unpacking SAFETY AND INSTALLATION English Français Deutsch Italiano Español CONTACT INFORMATION SPECIFICATION INSTALLATION TECHNE WORKBENCH Home Screen Menu Bar Options Settings Instruments Programs Library About APPENDIX Template Programs Incremented/Decremented Time and Temperature Gradient REMOVING AND EXCHANGING BLOCKS...

  • Page 4: Introduction

    ® System (TERS ). This allows the TC-PLUS Satellite to achieve ramp rates of up to 5ºC/s and is also energy saving, resulting in reduced running costs. Purchase of this instrument conveys a limited non-transferable immunity from suit for the purchaser's own internal research and development and applied fields other than human in-vitro diagnostics under non-real-time thermal cycler patents of Applied Biosystems LLC.

  • Page 5: Before Use

    BEFORE USE Before using the TC-PLUS Satellite please make sure you have read this manual carefully. If there is any doubt relating to the proper use of this equipment, the staff at Bibby Scientific Ltd. or your supplier will be happy to assist you.

  • Page 6: Safety And Installation

    The instrument should be carried using both hands. Never move or carry the instrument when in use or connected to the mains electricity supply. 1. All Techne instruments are supplied with a power cable; this may be integral or plug-in. Before connecting the instrument to the mains electricity supply, check the voltage against the rating plate (located on the back of the unit).
  • Page 7 Notwithstanding the description and specification(s) of the instruments contained in the operator’s manual, Techne reserves the right to make such changes as it sees fit to the instruments or to any of the components.

  • Page 8: Français

    Porter l'appareil à deux mains. Ne jamais déplacer ou transporter l’appareil lorsqu’il est en fonctionnement ou branché à l’alimentation électrique. 1. Tous les appareils Techne sont livrés avec un câble d'alimentation, qui peut être intégré à l'appareil ou à raccorder.
  • Page 9 Toute réparation sous garantie sera effectuée par le fournisseur. Le présent manuel a été exclusivement rédigé à l’attention des clients de la marque Techne et rien dans son contenu ne doit être pris comme une garantie, une condition ou une affirmation concernant la description, la commercialisation, l’adéquation à...

  • Page 10: Deutsch

    • SICHERHEIT DES BEDIENPERSONALS Alle Benutzer von Techne Geräten müssen Zugang zu der entsprechenden Literatur haben, um ihre Sicherheit zu gewähren. Es ist wichtig, daß diese Geräte nur von entsprechend geschultem Personal betrieben werden, das die in dieser Gebrauchsanweisung enthaltenen Maßnahmen und allgemeine Sicherheitsbestimmungen und - vorkehrungen beachtet.
  • Page 11 Unfall, Missbrauch oder Nachlässigkeit zurückzuführen ist. Wartungsarbeiten, die unter diese Garantie fallen, müssen von der Verkaufsstelle für dieses Gerät gehandhabt werden. Diese Anleitung wurde zur Information der Kunden von Techne erstellt und stellt in keinster Weise eine Gewährleistung, Bedingung oder Darstellung bezüglich der Beschreibung, Marktgängigkeit oder Zweckdienlichkeit dieser Geräte oder Bauteile dar.

  • Page 12: Italiano

    Bibby Scientific Ltd., la protezione fornita dall’apparecchiatura all’utilizzatore potrebbe essere a rischio. Tutte le unità Techne sono state progettate in conformità ai requisiti internazionali di sicurezza e sono equipaggiate con un interruttore anti surriscaldamento. Se si dovesse verificare qualche problema di sicurezza, disconnettere l’apparecchio dalla rete.
  • Page 13 L'assistenza secondo quanto stabilito dalla presente garanzia deve essere ottenuta dal fornitore dello strumento. Il presente manuale è stato preparato ad uso dei clienti di Techne e niente di quanto in esso contenuto costituisce garanzia, condizione o rappresentanza riguardo la descrizione, la commerciabilità, l'idoneità...

  • Page 14: Español

    • NO coloque un líquido directamente en el instrumento. SEGURIDAD DEL OPERARIO Todos los usuarios de equipos Techne deben disponer de la información necesaria para asegurar su seguridad. De acuerdo con las instrucciones contenidas en este manual y con las normas y procedimientos generales de seguridad, es muy importante que sólo personal debidamente...
  • Page 15 El distribuidor del instrumento proporcionará información sobre las reparaciones realizadas bajo esta garantía. Este manual se ha preparado con una finalidad informativa para los clientes de Techne y ninguna parte del manual se deberá considerar como una garantía, condición o reflejo con respecto a la descripción, comerciabilidad, idoneidad para un fin determinado o de otro tipo de la unidad o sus...

  • Page 16: Contact Information

    CONTACT INFORMATION For technical, sales or servicing information, contact your local Techne dealer or: North and South America Bibby Scientific Ltd. Bibby Scientific US Inc. t/a Techne Inc. Beacon Road, Stone 3 Terri Lane, Suite 10 Staffordshire, ST15 0SA Burlington, N.J. 08016 USA...

  • Page 17: Specification

    TC-PLUS SATELLITE SPECIFICATION TEMPERATURE Block temperature range 0 or 20ºC below ambient to 100ºC Block uniformity <±0.3ºC Temperature accuracy <±0.2ºC Gradient availability Across the full temperature range Maximum gradient 30ºC (16ºC with the 384-well block) Minimum gradient 1ºC Column uniformity with a 10ºC gradient <±0.3ºC...

  • Page 18: Installation

    See also the Safety Information sections on pages 6 to 15. Front view TC-PLUS Satellite ID and status indicator. Drawer release push panel. Power on/off Rear view Lifting Handle. Mains cable inlet USB port for connection to PC or TC-PLUS.
  • Page 19 Click on Workbench to download the thermal cycler PC software. Connect the TC-PLUS Satellite to the PC using the USB cable and switch the power ON using the switch located on the front of the unit. Launch the Techne Workbench PC software. Techne Workbench will automatically detect any...

  • Page 20: Techne Workbench

    TECHNE WORKBENCH HOME SCREEN Techne Workbench opens in the Library screen shown below. See the section LIBRARY on page 33 for further information on the features of this mode. MENU BAR OPTIONS Back and forward arrows Click on these to go back or forward between screens.

  • Page 21: Settings

    SETTINGS Click on Settings to select the default location on the PC for saving program and temperature log files and to set up preferred program defaults. Library Click on Add More Locations to set the default save location for programs and temperature log files. From the pop up box, select the required folder location then click OK.
  • Page 22 Once the location has been selected it will be listed on the screen as shown below. Further locations can be added in the same way. Delete locations by clicking on the cross next to the file path. User Preferences The user preferences can be used to set up temperature units, prompts during programming and to detect for software updates.
  • Page 23 Check for New Versions When set to ON, a message box will be displayed when a new version of Techne Workbench is available to download. Program Defaults Program defaults can be used to set up the preferred default settings for all newly created programs.
  • Page 24 Hot Start Set to ON to pause at the required set temperature. The program will not continue until the unit is manually un-paused. Final Extension Set to ON for the required hold time to add a final extension step to the program to ensure complete amplification of the products.

  • Page 25: Instruments

    Select Instruments from the menu bar to view all units connected to the PC. Identify To identify a particular TC-PLUS Satellite unit, click to select the required unit and click on Identify. The selected unit will emit a beeping sound and the display will flash.
  • Page 26 View Running Program. This will open the Live Monitor screen showing the progress of the program being run on the selected TC-PLUS satellite. Rename The Instrument The unit can be re-named. Select the required unit and click on Rename The Instrument.

  • Page 27: Programs

    PROGRAMS Click on Programs to create a new program, open an existing program or exit Techne Workbench. New Program Selecting this option opens the programming screen.
  • Page 28 View Clicking on this opens the Live Monitor screen which shows a graphical representation of the program. The run can also be started from this screen after selecting a unit. Save Saves the program using the name entered in the Program Name option (see below).
  • Page 29 Heated Lid Use this to set the heated lid temperature (in general approximately 10ºC higher than the hottest block temperature in a program). Set to ON to pre-heat the lid to the set temperature before the thermal Preheat Lid cycling program begins. Sample Cooling Set to ON to cool the block to 4ºC while the lid preheats.
  • Page 30 Time Enter the hold time of the step. For ease of use, the time can be entered in seconds. For example for 30 seconds type 30s or for 10 minutes type 600s. On moving to the next parameter box the time entry will auto- correct to the hh:mm:ss format.
  • Page 31 To add an increment/decrement temperature or gradient temperature to Increment/decrement temperature and gradient a step, click on the arrow key next to the Temperature parameter box. A prompt will show the maximum and minimum allowed limits. Enter the required value in the appropriate box. Click the arrow key again to close. A step modified with an increment/decrement time or gradient will be highlighted with an orange dot.
  • Page 32 Open Click on Open to browse for a program or temperature log file. Select the required program or temperature log file and click Open. Exit Click on Exit to close Techne Workbench.

  • Page 33: Library

    LIBRARY Click on Library to display all the programs and temperature log files stored on the PC. New Program Click on New Program to open the programming screen in order to create a new program. See the section New Program on page 27 for details of how to write a program. Open Click on the required file to select it and then click Open.
  • Page 34 Sort By Use this to organise the display order of the files. There are a number of sorting options. Sort by: Program Name Sorts the files alphabetically by program name. User Sorts the files alphabetically by user name. Recently used Sorts the files in the order of recent use.
  • Page 35 Search Use Search to find a specific program or temperature log file. Select the Search By … criteria you wish to use, then click on Apply Search. The screen will go back to the Library showing only the files which match the search criteria. To clear the search, click Clear Search. More than one type of search criteria can be applied to narrow down the number of files;...
  • Page 36 Search by File Type Search for only programs or temperature log files. Delete Use Delete to remove a file permanently. Select the file you wish to delete and click on Delete. A warning will be displayed asking for confirmation of the deletion. Click Yes to confirm the delete or Cancel if you do not wish to delete the file.
  • Page 37 Temperature Log Click on Temperature Log to view the Temperature Log Monitor screen. This displays the block temperature in 1 second intervals. Click on Program View to return to the Live Monitor screen.

  • Page 38: About

    ABOUT Click on About to check for Techne Workbench updates and to check the current version of software installed on the PC.

  • Page 39: Appendix

    APPENDIX TEMPLATE PROGRAMS To help with programming, a number of program templates are saved in Techne Workbench which are available to edit or can be run directly without changes. Details of the program templates are given below. Instrument and program defaults...

  • Page 40: Gradient

    GRADIENT The gradient feature of the TC-PLUS Satellite can be useful in optimising the annealing conditions for primers. A gradient can be set around a temperature in any step of a program. The set temperature is the temperature in the middle columns of the block and the gradient is the variation at the two extremes of the block;...

  • Page 41: Removing And Exchanging Blocks

    REMOVING AND EXCHANGING BLOCKS The thermal cycling block of the TC-PLUS Satellite can be removed and exchanged for one of a different format or for detailed cleaning. • First ensure that the unit is disconnected from the power supply and has been allowed to cool if previously in use.

  • Page 42: Faqs

    Do I need a different block for fully skirted plates? No. The TC-PLUS 96-well block will accommodate fully skirted as well as half-skirted and non-skirted PCR plates. What is “Sample Cooling”? Sample cooling can be applied during the lid pre-heat phase.

  • Page 43: Technical Support And Servicing

    E-mail: service@bibby-scientific.com. Phone: +44 (0)1785 810475 Fax: +44 (0)1785 810405 We are continually striving to improve our products and software. If you have any comments and suggestions on how we can do things better please send them to us at: techne@bibby-scientific.com.

  • Page 44: Additional Information

    ADDITIONAL INFORMATION USER MAINTENANCE Before cleaning your unit, disconnect it from the power supply. The outer case of the TC-PLUS Satellite may be cleaned with a cloth dipped in water or ethanol (hexane or 50% methanol can also be used). No part of the case or cover should be immersed in the solvents. Do not use aggressive solvents such as acetone or abrasive cleaners.

  • Page 45: Accessories

    The following consumables and accessories can be obtained from Bibby Scientific Ltd. Or your Techne distributor: Product code Description FTCPLUS/02/B TC-PLUS gradient block for 96 x 0.2ml micro tubes or plates FTCPLUS/05/B TC-PLUS gradient block for 60 x 0.5ml micro tubes FTCPLUS/384/B TC-PLUS gradient block for 384 well plates FTUB02TW 0.2ml micro tubes with domed caps (pack of 1000)

  • Page 46: Replacement Parts

    REPLACEMENT PARTS The following replacement parts can be obtained from Bibby Scientific Ltd. or your Techne dealer: Product code Description HH179(S) UK 230V mains lead with plug HH180(S) European mains lead with plug FCABLEUS US mains lead with plug 6205046...

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