Techne TC-412 Operator's Manual
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Techne TC-412 Operator's Manual

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TC-412
OPERATOR'S MANUAL
Issue 3
9/08

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Summary of Contents for Techne TC-412

  • Page 1 TC-412 OPERATOR'S MANUAL Issue 3 9/08...

  • Page 2: Table Of Contents

    Tubes or reaction vessels Switching on FRONT PANEL CONTROLS The LCD display Indicators The keys PROGRAMMING THE TC-412 Programming Options Option 1: Creating programs using templates Option 2: Creating a new program Example programs Programming To program increment or decrement temperature or time.

  • Page 3: Introduction

    Bibby Scientific Ltd or your supplier will be happy to assist you. Aspects of the PCR process are claimed in USA Patent Nos. 4,683,195; 4,683,202, and 4,965,188. Use of the TC-412 in such processes does not convey a licence top practice the processes themselves.

  • Page 4: Safety And Installation

    SAFETY AND INSTALLATION OPERATOR SAFETY Please read this manual carefully before using the thermal cycler. If the equipment is not used in the manner described in this manual the protection provided by the equipment might be impaired. All Bibby Scientific Ltd instruments are designed to conform to international safety requirements and are fitted with an over-temperature cut-out.
  • Page 5 rating plate (located on the back of the unit). Ensure that the voltage selector switch (located above the On/Off switch) is set to the appropriate voltage for the local supply. Please note that the unit must be earthed to ensure proper electrical safety. Connect the mains cable to a suitable plug according to the table below.
  • Page 6 Notwithstanding the description and specification(s) of the instruments contained in the operator's manual, Techne reserves the right to make such changes as it sees fit to the instruments or to any of the components.

  • Page 7: Sécurité Et Consignes D'installation

    SÉCURITÉ ET CONSIGNES D'INSTALLATION SÉCURITÉ DE L’OPÉRATEUR Lire attentivement le présent manuel avant d'utiliser le cycleur thermique. Si ce matériel n'est pas utilisé de la manière décrite dans le présent manuel, la protection fournie risque d'être compromise. Tous les appareils Bibby Scientific Ltd sont conçus pour être conformes aux exigences internationales de sécurité...
  • Page 8 1. Tous les appareils Bibby Scientific Ltd sont livrés avec un câble d'alimentation, qui peut être intégré à l'appareil ou muni d'une fiche. 2. Avant de raccorder l'appareil à l'alimentation électrique sur secteur, vérifier la tension par rapport à la plaque signalétique (située au dos de l'appareil).
  • Page 9 Dans le cadre de cette garantie, le service après-vente est à obtenir auprès du fournisseur de l'appareil. Le présent manuel a été préparé pour le confort des clients de Techne et rien dans son contenu ne doit être pris comme une garantie, une condition ou une affirmation concernant la description, la qualité...

  • Page 10: Sicherheits- Und Installationsinformationen

    SICHERHEITS - UND INSTALLATIONSINFORMATIONEN SICHERHEIT DES BEDIENPERSONALS Lesen Sie diese Anleitung vor Verwendung des Thermocyclers bitte sorgfältig durch. Wenn das Gerät nicht entsprechend der Bedienungsanleitung eingesetzt wird, können die Schutzfunktionen des Gerätes beeinträchtigt werden. Alle Geräte von Bibby Scientific Ltd entsprechen den internationalen Sicherheitsanforderungen und sind mit einer Übertemperatur-Schutzvorrichtung ausgestattet.
  • Page 11 1. Alle Geräte von Bibby Scientific Ltd werden mit einem Netzkabel geliefert, das entweder eingesteckt wird oder fest mit dem Gerät verbunden ist. 2. Vor dem Anschluss bitte kontrollieren, ob die Stromversorgung den Angaben auf dem Typenschild (auf der Geräterückseite) entspricht. Darauf achten, dass der Spannungswähler (über dem Ein/Aus-Schalter) auf die entsprechende örtliche Spannung eingestellt ist.
  • Page 12 Wartungsarbeiten, die unter diese Garantie fallen, müssen von der Verkaufsstelle für dieses Gerät gehandhabt werden. Diese Anleitung wurde zur Information der Kunden von Techne erstellt und stellt in keinster Weise eine Gewährleistung, Bedingung oder Darstellung bezüglich der Beschreibung, Marktgängigkeit oder Zweckdienlichkeit dieser Geräte oder Bauteile dar.

  • Page 13: Información De Seguridad E Instalación

    INFORMACIÓN DE SEGURIDAD E INSTALACIÓN SEGURIDAD DEL OPERARIO Lea atentamente este manual antes de utilizar el ciclador térmico. Si el equipo no se utiliza de la forma descrita en este manual, se reducirá la protección ofrecida por el equipo. Todos los instrumentos Bibby Scientific Ltd están diseñados para cumplir los requisitos internacionales de seguridad, e incluyen un dispositivo de corte de sobretemperatura.
  • Page 14 2. Antes de conectar el instrumento al suministro eléctrico, compruebe que el voltaje coincida con el indicado en la placa de régimen (situada en la parte trasera de la unidad). Asegúrese de que el interruptor de selección de voltaje (situado encima del interruptor de encendido/apagado) está ajustado al voltaje correcto del suministro eléctrico.
  • Page 15 Con independencia de la descripción y las especificaciones del instrumento que se indican en el manual del operario, Techne se reserva el derecho de realizar cambios en el instrumento o en cualquiera de sus componentes cuando lo estime oportuno.

  • Page 16: Informazioni Sulla Sicurezza E L'installazione

    INFORMAZIONI SULLA SICUREZZA E L'INSTALLAZIONE SICUREZZA DELL'OPERATORE Leggere attentamente il presente manuale prima di usare il ciclatore termico. Se non si usa l'apparecchiatura nel modo descritto nel presente manuale, la protezione fornita dall'unità potrebbe risultarne diminuita. Tutti gli strumenti Bibby Scientific Ltd sono progettati per rispettare i requisiti di sicurezza internazionali e sono montati con un sezionatore di sovratemperatura.
  • Page 17 2. Prima di collegare lo strumento all'alimentazione elettrica di rete, controllare la tensione confrontandola con la targhetta riportante i valori nominali (si trova sul retro dell'unità). Assicurarsi che il selettore di tensione (posto sopra l'interruttore On/Off) sia impostato sulla tensione corretta per l'alimentazione locale.
  • Page 18 L'assistenza secondo quanto stabilito dalla presente garanzia deve essere ottenuta dal fornitore dello strumento. Il presente manuale è stato preparato ad uso dei clienti di Techne niente di quanto in esso contenuto costituisce garanzia, condizione o rappresentanza riguardo la descrizione, la commerciabilità, l'idoneità...

  • Page 19: Before Switching On

    BEFORE SWITCHING ON Serial port Ensure that the voltage selector switch is set to the appropriate voltage for your supply; On/Off switch Ensure that the appropriate fuses are fitted for your supply: • For 230V set the voltage selector switch to 230V and fit T6.3A fuses. •...

  • Page 20: Tc-412 Specification

    TC-412 SPECIFICATION Temperature Temperature range 4°C to 99°C Temperature set point precision 0.1°C Block uniformity (over full range) ± 0.6°C Temperature accuracy ± 1.0°C End of program cooling below ambient Heating/Cooling Rate Heating rate, between 55°C and 90°C on a 50°C to 95°C step: 96 x 0.2 ml micro tubes...
  • Page 21 Running Programs Pause button Stop button End of program alarm Auto restart on power fail Interconnectivity Connection to PC control program Software updates from Techne web-site Dimensions Height 260mm Width 220mm Length 420mm Serial Port This socket is provided for RS232 PC link to run "Gensoft" the PC control software.

  • Page 22: User Operation And Consumables

    USER OPERATION AND CONSUMABLES HEATED LID To release the heated lid, lift the back of the handle as shown and use it to lift the lid. To close the lid, lower the lid onto the block containing the samples and press the handle down to shut. The silicone rubber mat supplied with the unit (Part No.

  • Page 23: Tubes Or Reaction Vessels

    TUBES OR REACTION VESSELS Techne does not recommend any specific tube or reaction vessel other than those described in this manual. We recommend using reaction volumes between 20 and 200µl. The tubes must withstand a pressure of 1 atmosphere at 100°C. Any vessel must be able to withstand the temperatures you are using without any danger of them deforming to the point where they fracture.

  • Page 24: Front Panel Controls

    Power: This indicator is lit when there is power to the TC-412. Status: This indicator is lit when the TC-412 is running a program. It is off when the unit is stopped. It will flash and beep slowly when the unit is paused. It will flash after a five second...

  • Page 25: The Keys

    THE KEYS The End, Finish or Exit key The "End" or "Finish" key is used to finish editing programs or to exit a program. It is also used to exit a sequence of key operations. The Up Arrow key The "Up Arrow" key is used for scrolling up through the lines on the screen.

  • Page 26: Programming The Tc-412

    Down arrow Enter Pause The following terms are used when programming the TC-412: Stage A 'Stage' is the name given to the main repeated part of a program containing the 'Steps' to be repeated. A program can contain a maximum of 25 stages although typically only one or two are used.

  • Page 27: Programming Options

    PROGRAMMING OPTIONS There are two options available for creating programs on the TC-412: 1. Copy and edit one of the pre-written templates. 2. Create a new program from the defaults. OPTION 1: CREATING PROGRAMS USING TEMPLATES There are two pre-written basic PCR templates: a two-step and a three-step template. These can be copied and modified as required.
  • Page 28 A new name can now be given to the copied UNNAMED # program (see below). Heated Lid 105°C Preheat Lid to edit To give a program a name To type in a name for the program, press UNNAMED # "Enter" and a cursor will appear in the Heated Lid 105°C...
  • Page 29 To edit a field or step in an existing program When the unit is switched on the display will Programs show this screen. Information If known, type in the 'Speed dial' number for Configuration the required program and press "Enter" to select This will take you directly to the required program.
  • Page 30 To edit a temperature, type in the required DONNA temperature. In the example here, the heated Heated Lid 105°C lid temperature is being edited. Preheat Lid Press "Enter" to accept. edit temperature Use the "Down Arrow" and/or the "Up to disable Arrow"...

  • Page 31: Option 2: Creating A New Program

    To copy a selected program If you select 'Copy program' a screen similar to UNNAMED # this will appear and the software will Heated Lid 105°C automatically give the program the next Preheat Lid number until it is named. to edit Press "Enter"...

  • Page 32: Example Programs

    EXAMPLE PROGRAMS A couple of example thermal cycling programs are shown below. Typical 3-Step PCR 3-Step PCR with multiple 'Stages' Heated Lid 105°C Heated Lid 105°C Preheat Lid Preheat Lid Pause Pause ------------------------- ------------------------- In denat 94°C 5min In denat 94°C 5min Hot Start Hot Start...
  • Page 33 A new stage will be inserted which consists of -------- Stage 1 -------- a 'Number cycles' field and one step. Number cycles Edit the number of cycles by typing the Step 94.0° C 00m30 number required then press "Enter" edit no.cycles Use the "Down Arrow"...
  • Page 34 To add a new stage before an existing one Use the "Up Arrow" or "Down Arrow" -------- Stage 1 -------- keys to highlight the stage number. Number cycles Press "Enter" Step 94.0° C 00m30 to insert stage A new stage will be added before the existing stage.

  • Page 35: To Program Increment Or Decrement Temperature Or Time

    To insert a ‘pause’ into a program To insert a pause after an existing stage, Step 94.0° C 00m30 follow the steps for inserting a stage as -------- Stage 2 -------- above. Number cycles Highlight the 'Number cycles' line then press Step 94.0°...
  • Page 36 The Hold Temperature of the incremented/decremented step is the defined Hold Temperature plus the summation of the increments/decrements. The first cycle is never incremented/decremented, only subsequent cycles. If you select a decrement, the Hold Temperature is prevented from falling below 4°C. If you select an increment, the Hold Temperature is prevented from rising above 99°C.
  • Page 37 The step will change to 'fst', meaning "first", Number cycles and the software will insert another line after Step 94.0° C 00m30 it entitled 'lst', meaning "last". 58.0° C 00m30 max Press "Enter" twice to go into edit mode. to edit to save Use the "Alpha/numeric"...
  • Page 38 Stopping what you are doing At any time during programming the TC-412, when you are satisfied with the program, you can finish and accept what you have done by first pressing the "End"...

  • Page 39: To Run A Program

    TO RUN A PROGRAM When the unit is switched on the display will Programs show this screen. Information Configuration to select If known, type in the 'Speed dial' number for Programs the required program and press "Enter" Information This will take you directly to the required Configuration program.
  • Page 40 During the run the LCD screen will report: Cycle 4/30 56m08 1. The cycle number Sample 58.0°C Hold @ 58.0°C 00m17 2. The time remaining (counting down in to STOP seconds) 3. The sample temperature (whether holding to PAUSE or ramping) for info 4.

  • Page 41: Information

    Program Information Pressing "Enter" while a program is DONNA running will give information about the Heated Lid current run including which program is Status ramping actually running and the unit status e.g. to exit holding or ramping. An example is shown here. INFORMATION The Information field on the front screen Programs...
  • Page 42 There are a number of options which can be Program defaults changed; these are listed here. Settings Use the "Up Arrow" and "Down Arrow" Passwords keys to select then press "Enter" to access Controller reset any of the options. Each option is discussed in detail below.
  • Page 43 The complete list of settings is given here. Set date x x / x x / x x Auto restart "on" will allow the unit to restart Set time x x : x x from where it left off should there be a power Date format failure and the power returns.

  • Page 44: Running The Tc-3000 From A Pc

    RUNNING THE TC-412 FROM A PC The TC-412 can be run from a PC using Techne’s Gensoft software. This is available to download from the Techne web-site at www.techne.com. You will need 'Winzip' (which is available free of charge on www.winzip.com) to unzip the files.

  • Page 45: Connecting Several Units To The Computer Using The Daisy Chain Cables

    CONNECTING SEVERAL UNITS TO THE COMPUTER USING THE DAISY CHAIN CABLES If you are driving two or more units then you will need part number FGEN485D, a 230V UK power pack set, or FGEN485E, a 230V European power pack set, or FGEN485P, a 120/100V USA power pack set. A power pack set includes: the connection to a computer (which also converts to RS485), a power pack, the necessary cables, a termination box and the software on disk with an instruction sheet.

  • Page 46: User Maintenance

    The case of the TC-412 may be cleaned with a cloth dipped in water or ethanol (methanol or formaldehyde can also be used). No part of the case or cover should be immersed in the solvents. Do not use aggressive solvents such as acetone or abrasive cleaners.

  • Page 47: Block Removal

    BLOCK REMOVAL Before removing the block, disconnect from the power supply and allow it to cool to below 50°C. ● Slide the quick release handles in the direction of the arrows indicated and lift the block assembly out of the unit. ●...

  • Page 48: Additional Information

    Please include details of the fault observed and remember to return the unit in its original packing. Techne accepts no responsibility for damage to units which are not properly packed for shipping: if in doubt, contact your supplier, giving the full serial number of the unit and software version number (shown when the unit is first switched on).

  • Page 49: The Heated Lid Over-Temperature Cut-Out

    Exchange the faulty fuse in the fuse holder for a working fuse of the correct value. Finally, replace the fuse drawer in the fuse compartment and push the drawer shut. Fuses which blow repeatedly indicate a serious fault and you should return the unit to your supplier for repair.

  • Page 50: Accessories

    0.5ml micro tubes thin wall Pack of 1000 FMIP384 384 well plate Pack of 50 REPLACEMENT PARTS The following replacement parts can be obtained from Techne or your Techne distributor: Item No Description Quantity required FCABRTUK Mains cable and plug, 230V UK...
  • Page 51 Thank you for reading this data sheet. Please contact us for a quotation: Head Office (UK) Switzerland Office P.O. Box 2124 Zugerstrasse 46 Chelmsford 6314 Unterägeri Essex CM1 3UP England Switzerland Tel: +44 (0)1245 600 560 Tel: +41 41 541 5877 Fax: +44 (0)1245 600 030 Fax: +41 41 541 5878 Email:...

This manual is also suitable for:

Tc-3000g

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