Subscribe to Our Youtube Channel
Related Manuals for Molecular Devices ClonePix 2
Summary of Contents for Molecular Devices ClonePix 2
- Page 1 ClonePix QUICK SET-UP INTRUCTIONS MANUAL SOFTWARE RELEASE 1.2.80.1071 07MAN1181.A1 Effective Date: 30-Jan-11 ECO #: 3093...
-
Page 2: Table Of Contents
MOLECULAR DEVICES > CLONEPIX 2 QUICK SET-UP INSTRUCTIONS Contents What are Quick Set-Up Instructions? ..........3 Starting up ClonePix 2 ................ 3 Loading Plates ..................4 Preparing for a Pick Run ..............5 The Pick Run ..................6 Imaging Settings ....................7 Picking Settings .................... -
Page 3: What Are Quick Set-Up Instructions
Turn on the compressor and ensure compressed air gauge is set to 80 psi (5.5 bar). Ensure Emergency Stop button is not pressed in. Switch on ClonePix 2. The HEPA filtration system works all the time that ClonePix 2 is on. After approximately 2 minutes, initiate ClonePix 2 software. -
Page 4: Loading Plates
MOLECULAR DEVICES > CLONEPIX 2 QUICK SET-UP INSTRUCTIONS Loading Plates It is important to know how to load plates into ClonePix 2 correctly. A ClonePix 2 bed layout is as shown below: Imaging station Source Cassette Destination Cassette Wash station The Source plate stacker system is located at the rear of the machine, and the Destination plate stacker system is at the front. -
Page 5: Preparing For A Pick Run
Orientation of destination plates in ClonePix 2 Preparing for a Pick Run Prior to carrying out a picking run, it is important to make sure that ClonePix 2 is set up correctly. The Prepare for Pick Run process is designed for this purpose. This process helps the user to validate that 1) the pins are firing correctly, 2) the camera, pins and microplates are aligned, and 3) the fluid system is sterile and ready for use. -
Page 6: The Pick Run
MOLECULAR DEVICES > CLONEPIX 2 QUICK SET-UP INSTRUCTIONS The Pick Run The Pick Run process is designed to aid new users through their first picking run. Use of the default parameters provided should facilitate successful imaging, colony selection and picking. -
Page 7: Imaging Settings
Barcode Options Select Read Barcode. Select Auto-assign barcode in case of failure. If there are no barcodes on the plates, ClonePix 2 will automatically assign a code. Batch plates Select this option. This assumes that multiple plates containing the same sample are being loaded and they will be processed as a single experiment. -
Page 8: Picking Settings
Barcode Options Select Read Barcode. Select Auto-assign barcode in case of failure. If there are no barcodes on the plates, ClonePix 2 will automatically assign a code. Deposit Options Leave unselected. Destination Plate Options Select Finish when cassette is empty. -
Page 9: Sanitize Pin Options
MOLECULAR DEVICES > CLONEPIX 2 QUICK SET-UP INSTRUCTIONS Sanitize Pin Options Leave as default – no changes required. Start Pick Run Click Start to begin the process. When prompted, load the cell colony plates into the source stacker cassette. Preview This is where image acquisition and colony detection settings are set up. - Page 10 My Configurations. Ensure that TransWL and FITC 1000ms options are selected. Note: Do not delete the Default option – it is required for other ClonePix 2 functions. Set the Prime Configuration to TransWL. This is critical for correct colony detection.
-
Page 11: Select Wells
Summary Click Next to proceed. Imaging ClonePix 2 will now automatically image the source plates, detect the colonies and generate combined data for all plates. While ClonePix 2 is imaging, pre-fill one or more 96-well destination plates with liquid medium (150 l recommended) and place in an incubator to equilibrate. -
Page 12: Results
MOLECULAR DEVICES > CLONEPIX 2 QUICK SET-UP INSTRUCTIONS Results Imaging results are displayed in the Results screen: Click on the Groups tab in the Graphs tab. Any group can be altered but this may compromise clonality and viability. Where possible it may be best to leave the groups with the default values. - Page 13 MOLECULAR DEVICES > CLONEPIX 2 QUICK SET-UP INSTRUCTIONS Colonies in the Ungated group are those that have good size, shape and distance from other colonies. To further isolate only the colonies in the Ungated group that have highest associated FITC fluorescence, click All Undiscarded Features to display all the colonies on the histogram.
-
Page 14: Picking Review
MOLECULAR DEVICES > CLONEPIX 2 QUICK SET-UP INSTRUCTIONS Picking Review In the Picking Review tab, select only the High FITC group: Under Sort Options, select Order By [FITC 1s] Exterior Mean Intensity. Click Next to proceed. Picking When prompted, load the destination plate(s) into the destination stacker cassette (the number of destination plates required will be displayed in the Pick Summary on the Picking Review tab). -
Page 15: Powering Down Clonepix 2
QUICK SET-UP INSTRUCTIONS Powering Down ClonePix 2 Exit from the ClonePix 2 application by selecting Exit from the File menu on the main setup screen. Close down Windows – Click the Start menu at the bottom of the screen then click Shut Down. -
Page 16: Contact Details
Information furnished by Molecular Devices (New Milton) Ltd is believed to be accurate and reliable; however, no responsibility is assumed by Molecular Devices (New Milton) Ltd, for its use; nor for any infringements of patents or other rights of third parties which may result from its use.
Need help?
Do you have a question about the ClonePix 2 and is the answer not in the manual?
Questions and answers