Table of Contents
Advertisement
Quick Links
Advertisement
Table of Contents
Related Manuals for WPI PUL-1000
Summary of Contents for WPI PUL-1000
- Page 1 WORLD PRECISION INSTRUMENTS INSTRUCTION MANUAL PUL-1000 Microprocessor-controlled micropipette puller (up to 4-step control*) Serial No._____________________ *The first three steps run only once, and the fourth step can run unlimited loops until the glass breaks. www.wpiinc.com 030321...
-
Page 3: Table Of Contents
PUL-1000 CONTENTS QUICK START ..........................IV ABOUT THIS MANUAL ........................1 INTRODUCTION ..........................1 Key Features ..........................2 Parts List ............................3 Unpacking ........................... 3 INSTRUMENT DESCRIPTION ......................4 Program Sequence ........................5 OPERATING INSTRUCTIONS ......................6 Quick Start Setup......................... 6 Load the Glass in the Carriages .................. -
Page 4: Quick Start
NOTE: Program sequence 00 is designed for OD 1.0 mm borosilicate capillary glass (WPI# 1B100-4), and sequence 00 is designed for OD 1.14 mm borosilicate capillary glass (WPI# 504949). Refer to the PUL-1000 Cookbook containing the installed program sequence. -
Page 5: About This Manual
NOTES and TIPS contain helpful information. Fig. 1 The PUL-1000 is programmable. INTRODUCTION PUL-1000 is a microprocessor controlled, up to four-stage, horizontal puller for making glass micropipettes or microelectrodes used in intracellular recording, patch clamp, microperfusion and microinjection. The puller was designed with tight mechanical specifications and precision electronics for complete control of the pulling process and accurate reproducibility. -
Page 6: Key Features
A glass capillary is heated by a platinum/iridium filament and pulled by a controlled force. The PUL-1000 is capable of producing a vast array of pipette shapes, such as a long graduated taper, a short graduated taperand a short taper (bee stinger). -
Page 7: Parts List
Concealed damage should be reported at once to the carrier and an inspection requested. Please read the section entitled “Claims and Returns” on page 19 of this manual. Please contact WPI Customer Service if any parts are missing at (941) 371-1003 or customerservice@wpiinc.com. -
Page 8: Instrument Description
Check the glass OD and install appropriate pads accordingly. Heating Filament–The PUL-1000 comes with a 2.5 x 2.5 mm platinum/iridium box filament (WPI #13834), which may be easily replaced, as needed. (See “Accessories” on page 14.) Glass Groove–Slide a single piece of capillary glass into the groove from one side or... -
Page 9: Program Sequence
“LOAD” on the display, use the arrows on the keypad to select a program. Refer to the PUL-1000 Cookbook for the details of the factory set programs and to pull the WPI pre-pulled tip types. You can start from these settings to get your own ideal program setting. -
Page 10: Operating Instructions
(Fig. 4). Fig. 4 PUL-1000 power supply cord Plug the power cord into the rear panel of the PUL-1000. Then, the switch illumi- nates (Fig. 5). Fig. 5 The power is on, and the PUL-1000 switch is illuminated. - Page 11 Lift the clamps and slide in the glass capillary clamp pads matching the outer diameter (OD) of the glass capillary. Lock a glass in the glass groove with the glass capillary clamps. Then recall a program. Refer to the PUL-1000 cookbook for details about the factory installed programs. Glass...
-
Page 12: Load The Glass In The Carriages
Load the Glass in the Carriages The glass capillary is held by clamps mounted on two movable carriages. Both carriages synchronously slide as a program is executed. Manually slide them back together when you are loading the glass. To open the clamps, place your thumb under the clamp and depress the release button with your index finger. -
Page 13: Running A Puller Program
PUL-1000 Running a Puller Program The top and bottom arrows on the keypad navigate numbers in increments of 1. The left and right arrows on the keypad navigate numbers in increments of 10. Fig. 8 Display setting options. Loading a Program Use the arrows to navigate to LOAD (00). -
Page 14: Programs
When the unit is powered on, the LCD screen displays the last used sequence in the window. PUL-1000 is designed to form the pipette in up to four steps. A basic program step (called a line) consists of four entries: The first stage is to pull a thin section on the capillary. -
Page 15: Program Parameters
PUL-1000 Program Parameters Fig. 9 The main display shows the parameters which may be controlled. For each step of a four-step sequence, the four primary parameters may be set. A description of each follows: HEAT–The HEAT parameter determines the amount of heat produced by the filament. -
Page 16: Glass Capillary Softening Test
(Fig. 10). NOTE: A force of 150 g is the factory set value for testing borosilicate glass capillaries using a 2.5 mm square box filament (WPI #13834). Fig. 10 The window displays when you press the STOP key. Mount a glass capillary on the carriage. See “Load the Glass in the Carriages” on page 8. - Page 17 PUL-1000 Set Screw Filament Clamp Filament Support Block Fig. 11 The set screw secures the Filament Clamp to the Filament Support Block. If the filament wears out or is damaged, it must be replaced. To replace the filament: Loosen the set screws that secures the Filament Clamp on the Filament Support Block.
-
Page 18: Choosing A Filament
Choosing a Filament Appropriate filament selection depends on your research application, but generally Box Filaments are recommended. This configuration is particularly suitable for slice preparations where long, parallel walls will aid penetration. If you are using a box filament, the size of the square box should be approximately 1.0mm to 1.5mm larger than the outside diameter of the glass to be pulled. -
Page 19: Troubleshooting
NOTE: If you have a problem/issue with that falls outside the definitions of this troubleshooting section, contact the WPI Technical Support team at (941) 371-1003 or wpi-ts@wpiinc.com. World Precision Instruments... -
Page 20: Appendix A: My Sequences
APPENDIX A: MY SEQUENCES Name: _____________________________________________ STEP HEAT FORCE DISTANCE DELAY Name: _____________________________________________ STEP HEAT FORCE DISTANCE DELAY Name: _____________________________________________ STEP HEAT FORCE DISTANCE DELAY Name: _____________________________________________ STEP HEAT FORCE DISTANCE DELAY World Precision Instruments... - Page 21 PUL-1000 Name: _____________________________________________ STEP HEAT FORCE DISTANCE DELAY Name: _____________________________________________ STEP HEAT FORCE DISTANCE DELAY Name: _____________________________________________ STEP HEAT FORCE DISTANCE DELAY Name: _____________________________________________ STEP HEAT FORCE DISTANCE DELAY World Precision Instruments...
- Page 22 World Precision Instruments...
-
Page 23: Warranty
WPI shall not be liable for any damage to data or property that may be caused directly or indirectly by use of this product. - Page 24 175 Sarasota Center Blvd., Sarasota FL 34240-9258 Tel: 941-371-1003 • Fax: 941-377-5428 • E-mail: sales@wpiinc.com 1 Hunting Gate, Hitchin, Hertfordshire SG4 0TJ Tel: 44 (0)1462 424700 • Fax: 44 (0)1462 424701 • E-mail: wpiuk@wpi-europe.com Germany Pfingstweide 16, D-61169 Friedberg (Hessen), Germany Tel: +49 (0)6031 67708-0 •...
Need help?
Do you have a question about the PUL-1000 and is the answer not in the manual?
Questions and answers