1. Manuals
  2. Brands
  3. DMT Manuals
  4. Medical Equipment
  5. 820MS
  6. User manual

DMT 820MS User Manual

Muscle strip myograph system
Hide thumbs Also See for 820MS:
Table of Contents

Advertisement

Quick Links

Download this manual
USER MANUAL, VOL.3.2
M Y O G R A P H S Y S T E M - 8 2 0 M S

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the 820MS and is the answer not in the manual?

Questions and answers

Related Manuals for DMT 820MS

Summary of Contents for DMT 820MS

  • Page 1 USER MANUAL, VOL.3.2 M Y O G R A P H S Y S T E M - 8 2 0 M S...

  • Page 3: Trademarks

    Changes may have been made to the software and hardware it describes since then. New information may be supplied separately. This documentation is provided with the DMT Muscle Strip Myograph System – Model 820MS Document Number: 820MS – UG2.2A All rights reserved. No part of this manual may be reproduced or transmitted in any form or by any means without the written permission of Danish Myo Technology A/S.

  • Page 4: Table Of Contents

    CHAPTER 4 - THE MUSCLE STRIP MYOGRAPH UNIT 4.1 Calibration of the Force Transducer 4.2 Checking the Force Transducer 4.3 Force Transducer Replacement 4.4 Myograph Maintenance 4.5 Cleaning the Myograph CHAPTER 5 - THE MOUNTING SUPPORTS AND MOUNTING IN THE 820MS CHAMBER APPENDIX 1 - SYSTEM SPECIFICATIONS...

  • Page 5: Safety

    SAFETY The Muscle Strip Myograph System - 820MS has been designed for use in teaching and research only. It is not intended for clinical or critical life-care use and should never be used for these purposes. The 820MS also should not be used for the prevention, diagnosis, curing, treatment, or alleviation of disease, injury, or handicap.

  • Page 6: Unpacking The Myograph System

    UNPACKING THE MYOGRAPH SYSTEM Take a few minutes to carefully inspect your new Muscle Strip Myograph System - 820MS for damage which may have occurred during handling and shipping. If you suspect any kind of damage, please contact DMT immediately and the matter will be pursued soon as possible.

  • Page 7: Ec Declaration Of Conformity

    EC DECLARATION OF CONFORMITY DMT A/S Certify and declare that the following apparatus: MUSCLE STRIP MYOGRAPH SYSTEM - MODEL 820MS Restrictive use: Only for laboratory use. Manufactured by: DMT A/S Skejbyparken 152 8200 Aarhus N. Denmark Conforms with the essential requirements of the EMC Directive 2004/108/EC.

  • Page 8: Chapter 1 - System Overview

    CHAPTER 1 - SYSTEM OVERVIEW 1.1 INTERFACE Heat indicator Valve buttons Myo-Interface display Power indicator Figure 1.1 Interface Front Panel...

  • Page 9: Interface Rear Panel

    1.2 INTERFACE REAR PANEL 4 Transducer connections Gas input pH meter connection Gas regulator (needle valve) Temperature probe Connection Power connector 4 Recorder outputs Grounding, (Analogue) USB output (Digital) connected to Data ON/OFF switch Acquisition Figure 1.2 Interface Rear Panel Vacuum input...

  • Page 10: Myodynamics Muscle Strip Myograph - 840Md

    1.3 MYODYNAMICS MUSCLE STRIP MYOGRAPH - 840MD Connection to Myo-Interface Figure 1.3 MyoDynamics Muscle Strip Myograph unit Micrometer Myograph clamp support connected to micrometer Force transducer pin Myograph clamp support connected to force transducer Figure 1.4 Close up of myograph unit...

  • Page 11: Chapter 2 - Setting Up

    CHAPTER 2 - SETTING UP 2.1 SETTING UP THE MUSCLE STRIP MYOGRAPH SYSTEM - 820MS DMT CS4/CS8 Stimulator (optional) PC data acquisition and analysis software (optional) PowerLab data acquisition system (optional) Myo-Interface front panel USB. DMT Device enabler option Connection to...

  • Page 12: Setting Up Step-By-Step

    Data acquisition is possible either by connecting the 620M Interface directly to a PC with Labchart Pro and the DMT Device enabler installed or through the BNC connectors and BNC cables from the 820M interface e.g. to the Powerlab box and the Powerlab box is then connected using a USB cable to the computer with Labchart installed(optional).
  • Page 13 Prior to the shipment of the Multi Wire Myograph 820M System, has gone through two days of continuous testing, including a final force transducer calibration. However, DMT recommends that a new force transducer calibration is performed before using the myograph system for the first time. The force transducer calibration procedure is...

  • Page 14: Chapter 3 - The Interface Menus

    CHAPTER 3 - THE INTERFACE MENUS Chapter 3 is a complete manual for the 820MS Interface. This chapter contains a detailed description of how to navigate the touch-screen menus and how to use the special features of the 820MS myograph. The 820MS system can be used in 2 different modes.

  • Page 15: Interface Menus - Calibrated Mode

    BNC outputs on the rear of the interface. The total voltage output from the BNC connectors is 2.5 volts. POWER-UP SCREEN After turning on the 820MS Interface, an “Introduction” screen appears. The system is auto-calibrating the A/D converters while this screen is displayed.
  • Page 16 Three menus are accessible from the default “Actual Force” screen or display. These menus are: Zero, Heat, and Settings. ZERO MENU This menu is used to zero the output of the transducers. When using a data acquisition program like LabChart by ADInstruments ®, using this feature will reset the baseline of the chart traces without affecting the calibrations or physically changing any pre-load tensions placed on the mounted vessels.
  • Page 17 SETTINGS MENU The “Settings Menu” contains several sub-menus that can be accessed to change functional aspects of the interface. These sub-menus include: FORCE CALIBRATION pH CALIBRATION FORCE REC. OUTPUT MEASUREMENT RANGE INTERFACE SETTINGS These are the options that are present when the system is in Calibrated Mode.
  • Page 18 The calibration procedure is listed in 6 individual steps and needs to be performed for each channel or transducer when calibrating the system. Step 1 involves setting up the chamber for calibration. Make sure the chamber contains the clamp support. Fill the chamber with double- distilled water for the volume to be used experimentally.
  • Page 19 The pH Module in the 820M is locked by a code. If the screen shown turn up, a code is needed from DMT to unlock the pH module. By ordering the pH sensor from DMT the pH sensor is delivered with the unlocking code and a quick guide how to do it.
  • Page 20 LOGIN  CODE  TO  pH plug-in port marked PH on the back side of the 820M interface. Call  DMT  A/S  to  get  acces  code. The pH electrode can be ordered at DMT by contacting your sales representative or emailing sales@dmt.dk. System  ID  number.  1056  ...
  • Page 21 Step 4 - The calibration is now finished. The values in the two bottom pH  CALIBRATION lines are the actual pH and temperature reading. Step  no.: The  pH  electrode  is  now  calibrated if  OK,  go  to  next  step,  else repeat  the  calibration.  ...
  • Page 22 Measurement Range The MEASUREMENT RANGE sub-menu in SETTINGS determines the maximum force capacity of the transducer. The factory setting is 200 mN, but the transducer capacity can be changed to 400 mN, 800 mN or a maximum of 1600 mN of force detection, depending on the size of the vessel used.
  • Page 23 exact defined setpoint. The exact temperature in the bath can be measured using the external temperature probe. Therefore, the user can adjust the temperature of each chamber individually to fine-tune the temperature setting so that EXACT temperatures can be achieved for any particular chamber. This is referred to as a temperature offset (TEMP OFFSET ON CHAMBER).
  • Page 24 Force  in  mN: SELECT The contraction force can be shown in gram or in mN on the touch screen and for Labchart Pro and DMT device enabler users the selected force unit will be shown automatically in   Labchart as well. Users of Powerlab and the analog signal from...
  • Page 25   ENTER   NOTE: For DMT device enabler users (no Powerlab) it is very important to restart Labchart Pro after changing the force units otherwise the force units will NOT be transferred to Labchart. Factory Diagnostics Entering FACTORY DIAGNOSTICS will display the LOGIN CODE TO DIAGNOSTICS window.
  • Page 26 In this menu the active myograph menu is marked by bold letters. To change to one of the other systems press SELECT and the text line will turn blue and then press ENTER. The text line will not be in bold letters indication that this systems menu structure is selected.

  • Page 27: Chapter 4 - The Muscle Strip Myograph Unit

    CHAPTER 4 - THE MUSCLE STRIP MYOGRAPH UNIT Chapter 4 contains a complete explanation of how to calibrate and maintain the 820MS myograph to ensure the equipment is always performing at peak performance. This Chapter also covers the mounting supports and how to mount a muscle strip in the 820MS chamber.
  • Page 28 depending on the temperature regulation in the room the myograph is set up in. Obviously, lower temperatures take less time and higher temperatures take more time to reach. Make sure adequate time is allowed so that calibration can be performed at the temperature at which the experiments will be performed.

  • Page 29: Checking The Force Transducer

    AND the transducer cannot be recalibrated, then the force transducer is broken and needs to be replaced. If any other problems related to the force transducer are encountered, please contact DMT for advice or further instructions.

  • Page 30: Force Transducer Replacement

    4.3 FORCE TRANSDUCER REPLACEMENT If the force transducer breaks and needs to be replaced, follow this step-by-step replacement procedure carefully: Remove the clamp from the transducer pin coming out of the transducer house. Disconnect the Myograph Chamber from the Interface. Turn the Myograph Chamber upside down and remove the transducer housing by loosening the two screws (A+B) as illustrated in “Figure 4.4”...
  • Page 31 IMPORTANT: CALIBRATE THE NEW FORCE TRANSDUCER BEFORE PERFORMING A NEW EXPERIMENT, AS DESCRIBED IN “CHAPTER 3 - THE INTERFACE MENUS” ON PAGE 17 AND “4.1 CALIBRATION OF THE FORCE TRANSDUCER” ON PAGE 27. Figure 4.5 The transducer in the transducer housing and close-up of transducer pin inside the transducer The arrow indicate the place that the grease needs to be applied to prevent water and buffer from damaging the transducer.

  • Page 32: Myograph Maintenance

    4.4 MYOGRAPH MAINTENANCE The Muscle Strip Myograph System - 820MS is a very delicate and sophisticated piece of research equipment. DMT recommends that the following sections are read carefully and that the instructions are followed at all times. MYOGRAPH CHAMBER TUBING To prevent the tubing from becoming blocked with buffer salt deposits after an experiment, remove the chamber cover from the Myograph Chamber.

  • Page 33: Cleaning The Myograph

    Figure 4.6 The areas where linear slide grease may be applied for smooth micropositioner movement 4.5 CLEANING THE MYOGRAPH DMT strongly recommends that the myograph chambers and surrounding areas are cleaned after each experiment. At the end of each experiment, use the following procedure to clean the myograph chambers and supports: Fill the myograph chamber to the edge with an 8% acetic acid solution and allow it to work for a few minutes to dissolve calcium deposits and other salt build-up.
  • Page 34 solution for about 1-2 minutes. Wash the myograph chamber and supports several times with double distilled water. If acids such as 1M HCl and 3M HNO are used to clean the chambers, make sure ALL surfaces are thoroughly dried after copious washes with double distilled water. Any residual acid will cause corrosion of the stainless steel chamber and/or mounting supports.
  • Page 35 IMPORTANT: IN EXCEPTIONAL CASES, THE SUPPORTS (CLAMP) MAY NEED TO BE REMOVED FROM THE MYOGRAPH CHAMBER AND CLEANED INDIVIDUALLY TO ASSURE PROPER CLEANING OF ALL SUPPORT SURFACES. NEVER SOAK THE SUPPORTS IN ANYTHING STRONGER THAN 8% ACETIC ACID FOR EXTENDED PERIODS OF TIME (I.E.

  • Page 36: Chapter 5 - The Mounting Supports And Mounting In The 820Ms Chamber

    CHAPTER 5 - THE MOUNTING SUPPORTS AND MOUNTING IN THE 820MS CHAMBER Each chamber contains supports with mounting clamps to facilitate the mounting of muscle strips in the myograph chambers. These clamps have been designed to optimally “clamp” the tissue without slipping during a contraction.
  • Page 37 Turn the upper wings back into position so that the muscle strip is clamped between the wings and base of the mounting clamp. Secure the screws. IMPORTANT: BE VERY CAREFUL WHEN TIGHTENING THE SCREW ON THE TRANSDUCER SIDE. EXCESSIVE PRESSING DOWN ON THE SCREW OR EXCESSIVE TORQUE FORCE WILL DAMAGE THE FORCE TRANSDUCER.

  • Page 38: Appendix 1 - System Specifications

    APPENDIX 1 - SYSTEM SPECIFICATIONS SPECIFICATIONS: Vessel size 3 mm - 19 mm Chamber Four individual chambers Chamber material Acid-resistant stainless steel Chamber volume Max. 8 ml Chamber suction Manual or automatic, time controlled, user defined Chamber cover Supplied with connections for gassing Chamber gassing Individually controlled per chamber by needle valved Force range...
  • Page 39 Danish Myo Technology A/S DMT-USA,Inc. E-mail: sales@dmt.dk E-mail: sales@dmt-usa.com Tel.: +45 87 41 11 00 Tel.: +1 734 707 0250 Fax: +45 87 41 11 01 Fax: +1 678 302 7013 DMT-China DMT-Asia Pacific Tel.: 86-21-6486 9685 Tel.: +61 2 8814 1597...

Table of Contents