Olympus SZX-RFL3 Instructions Manual
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Olympus SZX-RFL3 Instructions Manual

Olympus SZX-RFL3 Instructions Manual

Fluorescence attachment
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INSTRUCTIONS
SZX-RFL3
FLUORESCENCE ATTACHMENT
This instruction manual is for the Olympus SZX-RFL3 Fluorescence Attachment. To ensure the safety,
obtain optimum performance and familiarize yourself fully with the use of this equipment, we recommend
that you study this manual thoroughly before operating the microscope. Retain this instruction manual in an
easily accessible place near the work desk for future reference.
A X 9 9 0 1

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  • Page 1 SZX-RFL3 FLUORESCENCE ATTACHMENT This instruction manual is for the Olympus SZX-RFL3 Fluorescence Attachment. To ensure the safety, obtain optimum performance and familiarize yourself fully with the use of this equipment, we recommend that you study this manual thoroughly before operating the microscope. Retain this instruction manual in an easily accessible place near the work desk for future reference.

  • Page 2: Table Of Contents

    SZX–RFL3 CONTENTS Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going to assemble the microscope yourself, please read section 4, “ASSEMBLY” (pages 8 to 12) carefully. IMPORTANT - Be sure to read this section for safe use of the equipment. - 1-2 MODULE NOMENCLATURE CONTROLS OPERATION...
  • Page 3 2. The ultrahigh pressure mercury burner used should be the USHIO2D DC burner (mfd. by Ushio Inc.) or the HBO103W/2 (mfd. by OSRAM) that Olympus supplies. 3. Ensure that the burner is installed and that connection cords are connected properly before operating the unit.
  • Page 4 SZX microscope body.) 4. Always use the power cord provided by Olympus. Before plugging the power cord, make sure that the main switch of the power supply unit is set to “...
  • Page 5 MODULE NOMENCLATURE }If you have not assembled the microscope system, please see Chapter 4, “ASSEMBLY”. Lamp housing U-LH100HGAPO U-LH100HG Fluorescence Illuminator SZX-RFA3 ² SZX Zoom Microscope Body ³ SZX-ZB12 Objective DFPLAPO1.2XPF DFPLAPO1XPF Power supply unit (for 100 W mercury burner) Base *1.
  • Page 6 SZX–RFL3 CONTROLS Collector lens focus adjustment knob (Page 14) Excitation filter slider (Page 8/16) Mirror focus adjustment screw (rear panel) (Page 15) Filter turret (Page 5) Lam centering knobs (Page 14) Brightness iris diaphragm lever (Page 5) Shutter slider (Page 5) Pulled out: Shutter IN.

  • Page 7: Operation

    OPERATION }The weight of the fluorescence attachment makes focusing knob operation heavy. Use the optional SZX-CSP counterbalancer to make the operation lighter. Fluorescent Light Observation (Figs. 1 - 3) ³ … † 1. Pull the shutter slider @ out to the “Shutter IN” position. 2.

  • Page 8: Fluorescence Filter Combinations And Applications

    SZX–RFL3 Fluorescence Filter Combinations and Applications Displayed Excitation Applications Model Barrier Filter Name Filter SZX-FU BP330-385 BA420 BFP & U-excited observations. SZX-FBV BP400-440 BA475 CFP & BV-excited observations CFP separation & BV-excited SZX-FCFPA CFPA BP400-440 BA470-495 separation observations. SZX-FGFP BP460-490 BA510IF GFP &...
  • Page 9 3. SZX-FCFPA 4. SZX-FGFP 5. SZX-FGFPA 6. SZX-FG 7. SZX-FRFP1 8. SZX-FREP2...

  • Page 10: Assembly

    SZX–RFL3 ASSEMBLY }Please have your local Olympus representative perform the assembly work. 4-1 Assembly Diagram The following diagram shows how to install the modules, and the numbers indicate the sequence of installation. The items enclosed in are detailed on the subsequent pages.
  • Page 11 4-2 Detailed Assembly Procedures Attaching the Body Mount (Fig. 5) 1. Remove the cap @ on the focusing block by hooking a pointed material in the notch on the cap. 2. Loosen the dovetail mount clamping screw inside the cap of the focus- ing cap by turning the provided Allen screwdriver two or three times (coun- terclockwise).
  • Page 12 SZX–RFL3 4. Tighten the two stop screws … on the body mount firmly using the Allen wrench (small). 5. Using the Allen screwdriver, tighten the tension screw † while lightly push- † … ing it downward. Š 6. Rotate the zooming knob on the right side panel of zoom microscope body toward the front, to the minimum zoom position.
  • Page 13 Attaching the Mercury Burner (Figs. 10 & 11) 1. Loosen the socket clamping screw @ using the Allen screwdriver. 2. Hold the upper section of lamp housing and pull it upward to remove the socket section. ² # To prevent malfunctions, do not hold the lamp housing by the center- ing knobs ².
  • Page 14 ” (OFF) before connecting the power cord. ² Always use the power cord provided by Olympus. If no power cord is ³ provided, please select the proper power cord by referring to “PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of this instruction manual.

  • Page 15: Adjustments And Other

    ADJUSTMENTS AND OTHER Centering the Mercury Burner Turning Power ON Set the main switch to “ I ” (ON). The arc will stabilize in 5 to 10 minutes after ignition. Push the shutter slider into the “Shutter IN” position. }The mercury burner may not be ignited from the beginning due to variance between individual products. In this case, set the main switch to “...
  • Page 16 SZX–RFL3 4. Project the arc image on the stage plate by turning the collector lens ƒ focusing knob ƒ. (A) … If the arc image is not protected, adjust the burner centering knobs …. 5. Ring the arc image on the center of the upper (or lower) half of the field by turning the burner centering knobs ….

  • Page 17: Mounting The Nd Filter

    Precise Centering of the Mirror Arc Image }The mirror arc image position has been adjusted and fixed at the factory. Perform the centering of the mirror arc image after completing the center- † ing of the mercury burner and only when you want to make your adjust- ments very strict and precise.

  • Page 18: Using The Dummy Filter

    SZX–RFL3 Using the Dummy Filter }The dummy filter sliders can be used in fluorescence observation by attaching a filter. Dummy excitation filter slider Dummy barrier filter slider 1. Remove the ring spring then the light shield. 1. Insert the required barrier filter (20 mm dia., 6 mm or less 2.

  • Page 19: Proper Selection Of The Power Supply Cord

    If no power supply cord is provided, please select the proper power supply cord for the eqipment by referring to “ Specifications ” and “ Certified cord ” below: CAUTION: In case you use a non-approved power supply cord for Olympus products, Olympus can no longer warrant the electrical safety of the eqipment.
  • Page 20 SZX–RFL3 Table 2 HAR Flexible Cord APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS Alternative Marking Utilizing Printed or embossed Harmoniza- Black-Red-Yellow Thread tion Marking (May be located on Approval Organization (Length of color section in mm) jacket or insulation of internal wir- ing) Black Yellow...
  • Page 21 MEMO...
  • Page 22 2-43-2,Hatagaya, Shibuya-ku, Tokyo, Japan Postfach 10 49 08, 20034, Hamburg, Germany 2 Corporate Center Drive, Melville, NY 11747-3157, U.S.A. 491B River Valley Road, #12-01/04 Valley Point Office Tower, Singapore 248373 2-8 Honduras Street, London EC1Y OTX, United Kingdom. 104 Ferntree Gully Road, Oakleigh, Victoria, 3166, Australia This publication is printed on recycled paper.

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