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INSTRUCTIONS
SZX-RFL2
COAXIAL FLUORESCENCE ATTACHMENT
This instruction manual is for the Olympus SZX-RFL2 Coaxial Fluorescence Attachment. To ensure safety,
obtain optimum performance, familiarize yourself fully with the use of this equipment, we recommend that
you study this manual thoroughly before operating the microscope. Retain this instruction manual in an
easily accessible place near the work desk for future reference.
A X 9 7 5 1
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Summary of Contents for Olympus SZX-RFL2
- Page 1 SZX-RFL2 COAXIAL FLUORESCENCE ATTACHMENT This instruction manual is for the Olympus SZX-RFL2 Coaxial Fluorescence Attachment. To ensure safety, obtain optimum performance, familiarize yourself fully with the use of this equipment, we recommend that you study this manual thoroughly before operating the microscope. Retain this instruction manual in an easily accessible place near the work desk for future reference.
- Page 2 1. This equipment consists of precision instruments. Handle it with care and avoid subjecting it to sudden or severe impact. 2. The ultrahigh pressure mercury burner used should be the USH102D DC burner (mfd. by Ushio Inc.), that Olympus supplies.
- Page 3 SZX microscope body.) 4. Always use the power cord provided by Olympus. Before plugging the power cord, make sure that the main switch of the power supply unit is set to “...
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Page 4: Table Of Contents
SZX–RFL2 CONTENTS MODULE NOMENCLATURE CONTROLS ASSEMBLY OPERATION Turning on the Power Supply Unit ......................... 9 Centering the Mercury Burner ............................. 9 OBSERVATION Coaxial Reflected Fluorescence Observation .................. 11 Configuration of Mirror Units ..........................12 Spectral Characteristics of Filters ......................12 Brightfield Observation ................................ - Page 5 MODULE NOMENCLATURE Lamp housing U-ULH Burner socket U-ULS100HG Coaxial Fluorescence Illuminator SZX-RFA Collector lens Zoom microscope body IX-UCLHG/XE SZX-ZB12 Objective DFPLAPO1.2XPF Power supply unit (for 100W mercury burner) CONTROLS Coaxial Fluorescence Illuminator SZX-RFA Lamp housing Observation tube mount Illuminator tube mount Mirror units (one of 6 types) ·...
- Page 6 SZX–RFL2 Collector Lens Lamp Housing IX-UCLHG/XE U-ULH Collector lens focusing knob Burner Socket U-ULS100HG Burner centering knob Mercury burner USH102D Burner focusing knob Power Supply Unit (for 100W mercury burner) Burner ON LED AC receptacle Hour counter Main switch Output connector...
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Page 7: Assembly
# When the SZX-BS beam splitter is used together with this attachment, it is not possible to attach the SZX-SLR single lens reflex adapter onto the left side. The SZX-BS manufactured prior to February 1998 has a light path switch knob that is too long and interferes with the SZX-RFA. Contact your local Olympus representative for assistance. Mercury burner USH102D... - Page 8 SZX–RFL2 3-2 Detailed Assembly Procedures Attaching the Coaxial (Fig. 1) Fluorescence Illuminator 1. Remove the observation tube from the microscope. 2. Attach the coaxial fluorescence illuminator onto the microscope body by aligning the illuminator’s positioning groove @ with the microscope’s ²...
- Page 9 When Using the SZX7 Microscope Frame }The SZX-STF Focusing Adapter for Ring Mount makes it possible to build a system using the SZX-ZB7 (SZX7 microscope frame) + SZX-RFL2 (coaxial reflected fluorescent light illuminator) + SZX-ST/SZX-ILL series (SZX base series). The use of the SZX-STF improves the stability of the microscope and reduces oscillations, which pose a problem during fluorescent light imaging under extended exposure or during high-magnification observations.
- Page 10 SZX–RFL2 Assembling the Lamp Housing (Figs. 5 - 8) ² ³ Attaching the Mercury Burner 1. Remove the burner mount holder for transportation by loosening the burner clamping screws @ and ² (Fig. 5). (Also remove the old burner in case of burner replacement.) 2.
- Page 11 Resetting the Burner Hour Counter 1. Press the center of the reset button ² (Fig. 10) on the power supply unit’s front panel @ (Fig. 9) to reset the burner life indicator to 000.0. }The indicator shows elapsed time in hours. For safety’s sake, replace the burner when the indicator counts 200.0 hours.
- Page 12 2. Securely plug the burner socket’s connecting power cord ² into the power supply unit’s output connector ³. Always use the power cord provided by Olympus. If no power cord is † provided, please select the proper power cord by referring to the section “PROPER SELECTION OF THE POWER SUPPLY CORD”...
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Page 13: Operation
OPERATION # Overall precautions for observation 1. Verify that the power supply voltage and frequency match the requirements indicated on the name plate. 2. Make sure that the power cord and connecting cord are plugged in securely. 3. Engage the shutter if you interrupt observation for a short time. (Turning the mercury burner on and off (setting the main switch to “... - Page 14 SZX–RFL2 4. Screw in the burner focusing knob ƒ fully. 5. Using the burner centering knobs …, adjust so that two arc images of the … † specimen will be visible as shown in Fig. 16-A . # During centering with the minimum zoom magnification, the left side of the illuminated field may be vignetted.
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Page 15: Observation
OBSERVATION Coaxial Reflected Fluorescence (Figs. 17 – 19) ³ Observation }Use the black side of the stage plate as the stage intermediate seat. · If the stage glass of the transmitted illumination base is used, image may become hard to observe due to the autofluorescence. }If the reflection from the specimen surface makes viewing uncomfortable, attach the optional SZX-CCV glare shielding plate | onto the extremity of the objective and tighten the 2 clamping knobs ƒ. -
Page 16: Configuration Of Mirror Units
SZX–RFL2 Configuration of Mirror Units Display Plate Mirror Unit Dichroic Mirror Excitation Filter Absorption Filter Applications GFP and B excited observation, YFP observation SZX-MGFP DM505 BP460-490 BA510 IF # GFP band pass observation is not available. GFP observation, B excited pig- GFPA SZX-MGFPA DM505... -
Page 17: Brightfield Observation
SZX-MG SZX-MIY Brightfield Observation (Fig. 20) 1. Set the shutter slider ² to the pulled-out (light-excluding) position to cut the mercury burner illumination. 2. Set the mirror unit switch knob @ fully into the deepest position (indicated 3. Bring the specimen into focus and observe it. ²... -
Page 18: Proper Selection Of The Power Supply Cord
If no power supply cord is provided, please select the proper power supply cord for the equipment by referring to “ Specifications ” and “ Certified Cord ” below: CAUTION: In case you use a non-approved power supply cord for Olympus products, Olympus can no longer warrant the electrical safety of the equipment. - Page 19 Table 2 HAR Flexible Cord APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS Alternative Marking Utilizing Printed or Embossed Harmoniza- Black-Red-Yellow Thread (Length tion Marking (May be located on Approval Organization of color section in mm) jacket or insulation of internal wir- ing) Black Yellow...
- Page 20 MEMO...
- Page 21 MEMO...
- Page 22 Shinjuku Monolith, 3-1, Nishi Shinjuku 2-chome, Shinjuku-ku, Tokyo, Japan Wendenstrasse 14-18, 20097 Hamburg, Germany 3500 Corporate Parkway, Center Valley, Pennsylvania 18034-0610, U.S.A. 491B River Valley Road, #12-01/04 Valley Point Office Tower, Singapore 248373 31 Gilby Road, Mount Waverley, VIC., 3149, Melbourne, Australia 5301 Blue Lagoon Drive, Suite 290 Miami, FL 33126, U.S.A.
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