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104 . Spotlight 200 User's Guide
Analyzing Samples Using the ATR Imaging
Accessory
The ATR Imaging Accessory enables you to collect ATR spectra from an area of your sample
that you have identified using the microscope. Two nominal diameters are available for the
crystal in the accessory: 600 µm and 1200 µm. Using these large crystals, you can collect
spectra over a relatively wide area without lifting the crystal from the sample, as would be
necessary using the ATR Objective. This can help you obtain more reliable results, especially
if your sample is sticky and quickly contaminates the ATR crystal. It also reduces inaccuracies
with selecting the location to scan, due to the possibility that the sample could move each
time the crystal is raised and lowered.
Base
Pillar (b)
Anvil (d)
Mini-stage
adjuster (e)
Thumb pillar (f)
Force lever (g)
Clamp (i)
Figure 41 The ATR imaging accessory, showing the base (top) and the ATR crystal
arm (bottom)
The procedure described here assumes that you are familiar with using the ATR Imaging
Accessory. Further details of the installation and operation of the accessory are given in the
ATR Imaging Accessory User's Guide
(L1050002) supplied with the accessory.
NOTE: The collection and processing of ATR images described in the
User's Guide
requires PerkinElmer's SpectrumIMAGE software. If this software
application was not supplied with your Spotlight 200 system, follow the method
described here which uses Spectrum software to collect the spectra.
1. Remove the lower cassegrain.
Collecting the Spectrum of a Thick Sample
Refer to
2. Remove the dovetail connector underneath the stage (Figure 21).
3. Remove the ATR Objective crystal holder if it is fitted (page 87).
4. Move the stage to its lowest position.
(L1050048), which can be found on the
on page 67.
Pillar (a)
Support pillar
O ring (c)
Mini-stage
adjuster (e)
Thumb pillar (f)
Dust cover
Scribed line on
crystal arm (h)
Clamp (i)
Brake button
IR Manuals CD
ATR Imaging Accessory
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