Automated Atr Objective - PerkinElmer Spotlight 200 User Manual

100 . Spotlight 200 User's Guide
5. Click on the Stage View toolbar to collect an image survey.
6. Ensure that the ATR sampling mode is selected in the Setup Microscope Basic tab.
7. Use Monitor to find the most suitable stage height to give a staisfactory spectrum of
your sample.
Collecting a Sample Spectrum
Refer to
not change the stage height.
8. Place markers, lines or maps on to your image survey as required.
For information on how to do this, see the on-screen Help. The markers will all be set at
the stage height (Z-axis position) that you determined previously using Monitor.
9. Click the Scan Markers
10. When prompted, lower the ATR crystal to the working position and click Retry.
The stage will move to the position of each marker in turn and collect a spectrum. The
results will be displayed in an Image View.
Always retract the ATR crystal immediately after data collection has
been completed, in order to avoid accidental damage.
CAUTION

Automated ATR Objective

Collecting a Sample Spectrum
1. Select the ATR sampling mode on the Setup Microscope Basic tab.
2. Choose the Scan Settings you want on the Setup Instrument Basic tab.
3. Move the sample to the center of the Camera View.
Even though the microscope may not be focused on the sample, you can see a change
in the light intensity as the sample moves into the center of the field of view.
4. Look at the image in the Camera View pane and focus on the sample using the
Z-control on the joystick, or the Auto-Focus option, and then move the area of interest
into the center of the field of view.
5. Set the infrared aperture to a suitable size and rotation.
For information on how to do this see the on-screen Help.
The contact area of the ATR objective is approximately 100 µm in diameter. We
therefore recommend that you use a maximum aperture size of 100 x 100 µm.
6. Click to collect a background spectrum.
The stage moves downwards a small distance and the ATR crystal is lowered to collect
the background spectrum. Once complete, the crystal is raised and the stage returns to
its original position.
on page 98. Click Halt when completed, but do
button on the Measurement toolbar.