THORLABS LEDD3 Series Operation Manual
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THORLABS LEDD3 Series Operation Manual

THORLABS LEDD3 Series Operation Manual

Led illumination system for fluorescence lifetime imaging (flim)
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Operation Manual
Thorlabs Instrumentation
LED Illumination System for Fluorescence Lifetime Imaging
(FLIM)
LEDD3
2009

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Summary of Contents for THORLABS LEDD3 Series

  • Page 1 Operation Manual Thorlabs Instrumentation LED Illumination System for Fluorescence Lifetime Imaging (FLIM) LEDD3 2009...
  • Page 2 Version: Date: 03.03.2009 © 2009 Thorlabs © 2009 Thorlabs...

  • Page 3: Table Of Contents

    2 Certifications and Compliances ........................... 3 Technical Data ........................... Common Data ................................. 47 Technical Data ................................. 47 LED for Frequency Domain FLIM ................................. 48 4 Thorlabs 'End of Life' Policy (WEEE) ........................... Waste Treatment on your own Responsibility ................................. 52 © 2009 Thorlabs...

  • Page 4: List Of Acronyms

    Part VII Listings 1 List of Acronyms ........................... 2 List of Figures ........................... 3 Addresses ........................... Part VIII Application Note 1 Fluorescence Lifetime Imaging (FLIM) ........................... Time Domain FLIM ................................. 58 Frequency Domain FLIM ................................. 59 Index © 2009 Thorlabs...

  • Page 5: Foreword

    This manual contains "WARNINGS" and "ATTENTION" label in this form, to indicate danger for persons or possible damage of equipment. Please read these advises carefully! NOTE This manual also contains "NOTES" and "HINTS" written in this form. © 2009 Thorlabs...
  • Page 6 General Information LEDD3 - FLIM LED Driver User Manual Part...

  • Page 7: Part I General Information

    Fluorescence Lifetime Imaging (FLIM) and other microscopy applications that require modulated high-power LED illumination sources. The compact LED driver in the LEDD3 series can supply a high current (up to 1A) to high-power LEDs. The LEDD3 series system includes an optical head, modulating electronics, and a heat sink designed to dissipate the heat generated by high-power LEDs.
  • Page 8 If viewing the UV light is necessary, use UV light protective glasses to avoid damage by the UV light. GCautionG The head of the LEDD3 is also a heat sink for the LED and will be heated up to more than 50°C! © 2009 Thorlabs...
  • Page 9 Thorlabs GmbH. The correction of interference caused by such unauthorized modification, substitution or attachment will be the responsibility of the user.

  • Page 10: Ordering Codes And Accessories

    LEDD3 - FLIM LED Driver with LED Head @ 470nm LEDD3-630 LEDD3 - FLIM LED Driver with LED Head @ 630nm Other wavelength on request ranging from 365nm up to 940nm. Please visit our homepage http://www.thorlabs.com for further information. © 2009 Thorlabs...
  • Page 11 Getting Started LEDD3 - FLIM LED Driver User Manual Part...

  • Page 12: Part Ii Getting Started

    Turn the unit on by pressing the power switch on the rear side of the unit. After the device is powered up, the graphics display will show a 'Welcome' screen © 2009 Thorlabs...

  • Page 13: Physical Overview

    The LEDD3 is immediately ready to use after turning on. The rated accuracy is reached, however, after a warming-up period of approximately 10 minutes. Physical Overview 2.3.1 Operating Elements on the Front Panel Figure 2 Display and Operating Elements on the Front Panel © 2009 Thorlabs...

  • Page 14: Operating Elements On The Rear Panel

    LEDD3 - FLIM LED Driver User Manual 2.3.2 Operating Elements on the Rear Panel Figure 3 Operating Elements on the Rear Panel 2.3.3 Operating Elements on the LED Head Figure 3a Operating Elements on the LED Head © 2009 Thorlabs...

  • Page 15: Part Iii Operating The Ledd3

    Operating the LEDD3 LEDD3 - FLIM LED Driver User Manual Part...

  • Page 16: Operation And Settings

    Current, Internal Modulation or External Control Mode). The 'Main Menu' can be accessed via the 'ESC' button. Figure 5 Main Menu To select an operation mode or a configuration display the multi-control knob is used. Press the 'Ok' button to enter the selected item. © 2009 Thorlabs...

  • Page 17: Operation Modes

    To change the settings select one of the parameters using the multi-control knob and press the 'Ok' button. The corresponding screen appears and the value for the current, the frequency or the modulation depth can be adjusted with the multi-control © 2009 Thorlabs...
  • Page 18 Internal Modulation - Frequency Adjustment Figure 10 Internal Modulation - Modulation Depth Adjustment See the chapter Technical Data for information about cut-off frequencies for certain LEDs. The following diagram illustrates the definition of current and modulation depth: © 2009 Thorlabs...

  • Page 19: Figure 11 Definition Of The Parameter Current And Modulation Depth

    The last selected operation mode will be saved. After switching on the LEDD3 again this operation mode will be automatically the active mode. All settings are saved and validated after a shut off. The current settings in the 'Constant Current Mode' and in © 2009 Thorlabs...

  • Page 20: Settings And Configuration

    (LED fmax) can be set. Press the 'Ok' button to select one of these limits. The multi- control knob is used to change the value and has to be confirmed with the 'Ok' button or canceled with the 'ESC' button. © 2009 Thorlabs...

  • Page 21: Figure 15A Led Maximum Current

    'Brightness' setting can be accessed by pressing the 'Ok' button. The multi-control knob is used to change the brightness of the LCD backlight between 0 and 100%. Confirm your setting with 'Ok' or cancel it with 'ESC'. Figure 16 LCD Backlight Brightness Setting © 2009 Thorlabs...

  • Page 22: Remote Application

    3.2.1.1 The Installation Menu Before installing the LEDD3 remote application, please make sure that no Thorlabs LEDD3 - FLIM LED Driver is connected. After you inserted the LEDD3 installation CD an autorun menu will appear. If autorun is disabled on your system please browse the installation CD and run "CD-Drive:\Autorun\Autorun.exe".

  • Page 23: Figure 19 Visa Installation Start Screen

    VISTA©® you might be prompted to change to the "elevated mode". Please ask your administrator for help when you do not have administrator privileges. Press "Next" to continue. Figure 19 VISA Installation Start Screen Now you have to specify an installation path. Press "Next" to continue. © 2009 Thorlabs...

  • Page 24: Figure 20 Selection Of The Installation Path

    License Agreement In the next window please select the features to install. To operate the LEDD3 remote application you need at least the USB and the Serial feature (see figure 22). Confirm with "Next" to start installation. © 2009 Thorlabs...

  • Page 25: Figure 22 Selection Of Visa Features

    Select "LEDD3 - Application software" from the installation menu to start the installation wizard. If you are running Windows VISTA©® you might be promted to change to the "elevated mode", as shown in figure 24. Please ask your administrator for help when you do not have administrator privileges. © 2009 Thorlabs...

  • Page 26: Figure 24 Request For Administrator Privileges

    Confirm with "Next" when you selected the installation path of your choice. Figure 25 Selecting the Installation Paths During the installation process a separate installation wizard will start to register the device drivers on your system. © 2009 Thorlabs...

  • Page 27: Figure 26 The Device Driver Installer

    Please press 'Next >' and follow this wizard. You will be asked two times to "Continue anyway" the installation. Please do so. Figure 27 The Windows Logo Test After installation was successful you will see a window containing some information about the installed drivers. Press "Finish" to close the wizard. © 2009 Thorlabs...
  • Page 28 On the first connection of a device the "Found New Hardware Wizard" will start to install the new device. Depending on the configuration of your system you might be asked if you want to connect to "Windows Update to search for software" shown in © 2009 Thorlabs...
  • Page 29 30. Please select "No, not this time" and click "Next" to continue. Figure 30 The Found New Hardware Wizard Select "Install the software automatically" and click "Next" to continue. Figure 31 Choosing the Installation Mode Finalize the installation by clicking "Finish". © 2009 Thorlabs...

  • Page 30: Operating The Ledd3 By The Remote Application

    "USB" at the back of the main control. After starting the application the following window is displayed. Figure 33 The Start Screen Use the green button or the menu option 'Connect...' to open the device selection dialog. © 2009 Thorlabs...
  • Page 31 The maximum user frequency is stored in the LED head and is specific to the mounted LED. The maximum current is half of the user current limit, which is set in the configuration menu. © 2009 Thorlabs...
  • Page 32 LEDD3. The applied voltage corresponds to the LED current. 1V is equivalent to a LED current of 100mA. A maximum voltage of 10V can be applied, which will result in a current of 1A. Figure 37 The External Control Mode © 2009 Thorlabs...
  • Page 33 Configuring a new LED 3.2.2.6 The LEDD3 - FLIM LED Driver offers the possibility to operate a LED of your choice. Use the menu 'Configuration/LED Configuration' to set the new LEDs maximum current and frequency. Figure 40 LED Configuration Menu © 2009 Thorlabs...

  • Page 34: Changing The Led

    LEDD3 - FLIM LED Driver User Manual Figure 41 LED Maximum Limit Figure 42 LED Maximum Frequency Changing the LED The LEDD3 - FLIM LED Driver offers the possibility to operate a LED of your choice. Contact Thorlabs for more information! © 2009 Thorlabs...
  • Page 35 Computer Interface LEDD3 - FLIM LED Driver User Manual Part...

  • Page 36: Part Iv Computer Interface

    Please refer to the manuals, which are copied on your system when installing the device driver. Note: Please be aware that you need a VISA engine installed on your system to use the LEDD3 - VISA Instrument Driver. © 2009 Thorlabs...

  • Page 37: Command Reference

    Set Maximum Current Limit 4.3.2.3 Command: LEDD3_setMaxLimit Parameter: Maximum current limit in ampere Response: None Description: Sets the LEDs maximum current limit in ampere. This limit should only be set in case a new LED was installed. © 2009 Thorlabs...
  • Page 38 Sets the LED output. 4.3.2.10 Get LED OnOff Command: LEDD3_getLedOnOff Parameter: None Response: LED output state Description: Returns the LED output state. 4.3.2.11 Set Internal Modulation Current Command: LEDD3_setModuCurrent Parameter: Internal modulation current in Ampere Response: None © 2009 Thorlabs...
  • Page 39 Description: Sets the current in ampere used for the constant current mode. Get Constant Current 4.3.2.18 Command: LEDD3_getConstCurrent Parameter: None Response: Constant current in ampere Description: Returns the current in Ampere used for the constant current mode. © 2009 Thorlabs...
  • Page 40 Serial number Firmware revision Description: This function returns the device identification information. 4.3.2.24 Revision Query Command: LEDD3_revisionQuery Parameter: None Response: Instrument driver revision Firmware revision Description: This function returns the instruments driver revision and the devices firmware revision. © 2009 Thorlabs...

  • Page 41: Status Reporting

    OTP Head Changed The bit 'OTP Head' (Over Temperature Head) has changed. Bit 11 OTP Head Over temperature in the LED head was detected. The LED head was switched off. Bit 12 Interface Refresh The user has changed settings. © 2009 Thorlabs...

  • Page 42: Part V Maintenance And Repair

    Maintenance and Repair LEDD3 - FLIM LED Driver User Manual Part...

  • Page 43: Maintenance

    If necessary the unit and the display can be cleaned with a cloth dampened with water. The LEDD3 does not contain any modules that could be repaired by the user himself. If a malfunction occurs, the whole unit has to be sent back to Thorlabs. Do not remove covers! Firmware Update Firmware upgrades can be done by the user via the USB interface.
  • Page 44 · Is on of the three operation modes (Constant Current, Internal Modulation or External Control) selected? - An operation mode can be selected from the main menu using the multi- function knob and pressing the 'Ok' button. © 2009 Thorlabs...
  • Page 45 Appendix LEDD3 - FLIM LED Driver User Manual Part...

  • Page 46: Part Vi Appendix

    Thorlabs GmbH reserves the right to change this instruction manual or the technical data of the described unit at any time and without notice. © 2009 Thorlabs...

  • Page 47: Certifications And Compliances

    Safety requirements for electrical equipment for Compliance 1:2001 measurement, control, and laboratory use. Equipment Test and measuring Type Safety Class I equipment (as defined in IEC 60950-1:2001) Class Compliance demonstrated using high-quality shielded interface cables shorter than 3 meters. © 2009 Thorlabs...
  • Page 48 Emissions, which exceed the levels required by these standards, may occur when this equipment is connected to a test object. MOD IN port capped at IEC 61000-4-3 test. Performance Criterion C was reached at additional test levels according to EN 61326-1:2006 table 2 © 2009 Thorlabs...

  • Page 49: Technical Data

    Arbitrary (External Control Mode only) Trigger Input max. 10V (External Control Mode only) 1V corresponds to 100mA LEDD3-365 Nominal Wavelength 365nm Maximum Current 700mA Cut-off Frequency 90MHz LEDD3-405 Nominal Wavelength 405nm Maximum Current 1000mA Cut-off Frequency 95MHz © 2009 Thorlabs...

  • Page 50: Led For Frequency Domain Flim

    UV light can catch one's eye directly. If viewing the UV light is necessary, use UV light protective glasses to avoid damage by the UV light. The 10dB cut-off frequency of the LEDD3-365 is 90MHz. The maximum current is © 2009 Thorlabs...
  • Page 51 The LEDD3-405 contains the UV LED LZ1-00UA05 from LedEngin. The LED emits a wavelength at 405nm and an optical power of up to 1000mW. The 10dB cut-off frequency of the LEDD3-405 is 95MHz. The maximum current is 1000mA. Figure 45 Phase Difference of LEDD3-405 © 2009 Thorlabs...
  • Page 52 The LEDD3-630 contains the red LED LZ1-00R105 from LedEngin. The LED emits a wavelength at 630nm and an optical power of up to 100lm. The 10dB cut-off frequency of the LEDD3-630 is 70MHz. The maximum current is 1000mA. © 2009 Thorlabs...
  • Page 53 Appendix Figure 47 Phase Difference of LEDD3-630 © 2009 Thorlabs...

  • Page 54: Thorlabs 'End Of Life' Policy (Weee)

    Waste Treatment on your own Responsibility If you do not return an 'end of life' unit to Thorlabs, you must hand it to a company specialized in waste recovery. Do not dispose of the unit in a litter bin or at a public waste disposal site.

  • Page 55: Part Vii Listings

    Listings LEDD3 - FLIM LED Driver User Manual Part...

  • Page 56: List Of Acronyms

    Figure 21 License Agreement Figure 22 Selection of VISA Features Figure 23 Finalize Installation Figure 24 Request for Administrator Privileges Figure 25 Selecting the Installation Paths Figure 26 The Device Driver Installer Figure 27 The Windows Logo Test © 2009 Thorlabs...
  • Page 57 Figure 43 No VISA Engine installed Figure 44 Phase Difference of LEDD3-365 Figure 45 Phase Difference of LEDD3-405 Figure 46 Phase Difference of LEDD3-470 Figure 47 Phase Difference of LEDD3-630 Figure 48 Crossed out "Wheelie Bin" Symbol © 2009 Thorlabs...

  • Page 58: Addresses

    Email: sales@thorlabs.jp Web: www.thorlabs.jp Our company is also represented by several distributors and sales offices throughout the world. Please call our hotline, send an Email to ask for your nearest distributor or just visit our homepage http://www.thorlabs.com © 2009 Thorlabs...
  • Page 59 Application Note LEDD3 - FLIM LED Driver User Manual Part VIII...

  • Page 60: Part Viii Application Note

    The decay can be described with a simple -t/ t exponential function: I(t)=I(t Mostly lasers are used to generate pulses with several ps pulse width. Figure A1 Time Domain FLIM © 2009 Thorlabs...

  • Page 61: Frequency Domain Flim

    Furthermore, the intensity to the excitation and fluorescence sine waves will be modulated. The lifetime can be calculated from the modulation ratio of the intensity. Hence, the FLIM in the frequency domain provides 2 values for the lifetime. Figure A2 Frequency Domain FLIM © 2009 Thorlabs...

This manual is also suitable for:

Ledd3-365Ledd3-405Ledd3-470Ledd3-630

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