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Waters ACQUITY System Manual

Uplc h-class
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ACQUITY UPLC H-Class
System Guide
715005049
Copyright © Waters Corporation 2016
Revision C
All rights reserved

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  • Page 1 ACQUITY UPLC H-Class System Guide 715005049 Copyright © Waters Corporation 2016 Revision C All rights reserved...

  • Page 2: General Information

    This document is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, its use. For the most recent revision of this document, consult the Waters Web site (waters.com).

  • Page 3: Customer Comments

    We seriously consider every customer comment we receive. You can reach us at tech_comm@waters.com. Contacting Waters Contact Waters with enhancement requests or technical questions regarding the use, transportation, removal, or disposal of any Waters product. You can reach us via the Internet, telephone, or conventional mail. Waters contact information Contacting medium Information...

  • Page 4: Safety Hazard Symbol Notice

    Milford, MA 01757 Safety considerations Some reagents and samples used with Waters instruments and devices can pose chemical, biological, or radiological hazards (or any combination thereof). You must know the potentially hazardous effects of all substances you work with. Always follow Good Laboratory Practice (GLP), and consult your organization’s standard operating procedures as well as your local...

  • Page 5: Bottle Placement Prohibition

    Bottle placement prohibition Warning: To avoid injury from electrical shock or fire, and damage to the equipment, do not place vessels containing liquid atop the workstation or ancillary equipment or otherwise expose those units to dripping or splashing liquids. Prohibited: Do not place vessels containing liquid—such as solvent bottles—atop the workstation or ancillary equipment or otherwise expose those units to dripping or splashing liquids.

  • Page 6: Applicable Symbols

    Directive (WEEE) 2012/19/EU, contact Waters Corporation for the correct disposal and recycling instructions. Serial number Part number catalog number Audience and purpose This guide is intended for personnel who operate and maintain the ACQUITY UPLC H-Class system. August 8, 2016, 715005049 Rev. C Page vi...

  • Page 7: Intended Use

    Intended use Waters designed this system to perform liquid chromatography separations in these environments: • Pharmaceutical development and discovery • Quality assurance and quality control • Chemical materials • Environmental • Food safety The system is not intended for use in diagnostic applications.

  • Page 8: Ism Classification: Ism Group 1 Class B

    Class B products are suitable for use in both commercial and residential locations and can be directly connected to a low voltage, power-supply network. EC authorized representative Address Waters Corporation Stamford Avenue Altrincham Road Wilmslow SK9 4AX UK Telephone...

  • Page 9: Table Of Contents

    ISM classification: ISM group 1 class B..................viii EC authorized representative ......................viii 1 ACQUITY UPLC H-Class system ................14 1.1 UPLC technology .......................... 14 1.2 Features of the ACQUITY UPLC H-Class system ................ 16 1.2.1 Software features......................... 17 1.3 System components........................18 1.3.1 Quaternary solvent manager ....................20 August 8, 2016, 715005049 Rev.
  • Page 10 1.3.2 Sample manager-flow through needle................. 20 1.3.3 Column heater ........................21 1.3.4 Column manager (optional) ....................21 1.3.5 Sample organizer (optional)....................22 1.3.6 Detection..........................22 1.3.7 Local Console Controller (optional) ..................23 1.3.8 FlexCart (optional) ....................... 23 1.3.9 Column technology ......................23 1.4 For additional information......................24 2 Performance optimization..................26 2.1 General guidelines ........................
  • Page 11 3.5.8 TUV control panel ........................ 58 3.5.9 2432 conductivity control panel ................... 59 3.6 Starting up the system ........................60 4 System maintenance ....................63 4.1 Contacting Waters Technical Service ..................63 4.1.1 Viewing module information....................63 4.2 Maintenance procedures and frequency..................64 4.3 Spare parts............................ 64 4.4 Troubleshooting with Connections INSIGHT ................64 4.4.1 To submit a Connections INSIGHT request: ...............
  • Page 12 A.1.1 Specific warnings ........................ 76 A.2 Notices............................77 A.3 Bottles Prohibited symbol ......................77 A.4 Required protection ........................78 A.5 Warnings that apply to all Waters instruments and devices ............78 A.6 Warnings that address the replacing of fuses................82 A.7 Electrical symbols ......................... 84 A.8 Handling symbols ......................... 85 B Solvent considerations....................86...
  • Page 13 B.12 Quaternary solvent manager recommendations................. 91 B.13 Sample manager - flow through needle recommendations ............92 B.14 Common solvent properties......................92 B.14.1 Solvent miscibility ......................94 B.14.2 Solvent stabilizers ......................95 B.14.3 Solvent viscosity........................ 95 B.14.4 Wavelength selection ......................96 C Specifications......................101 C.1 System specifications ......................... 101 C.1.1 Instrument control specifications..................102 C.1.2 Environmental specifications.....................

  • Page 14: Acquity Uplc H-Class System

    In 2004, Waters made significant advances in instrumentation and column design to introduce UPLC technology to the field of separation science. By employing this technology, Waters’ ACQUITY UPLC systems achieve a marked increase in resolution, speed and sensitivity in liquid chromatography when compared to conventional systems.
  • Page 15 Figure 1–1: Evolution of particle size in liquid chromatography and the impact on separation efficiency It is apparent from the figure, above, that using 1.7-µm particles achieves higher efficiency that persists as flow rate increases (lower HETP indicates higher efficiency). When operating in this area of the plot, the peak capacity and the speed of a separation can set limits well beyond those of conventional HPLC technology.

  • Page 16: Features Of The Acquity Uplc H-Class System

    1.2 Features of the ACQUITY UPLC H-Class system The ACQUITY UPLC H-Class system combines the speed and performance of UPLC with the ability to run HPLC separations. This combination provides many benefits, including these: • High-pressure, small-particle chromatography allowing faster, higher-resolution analyses, compared with conventional HPLC •...

  • Page 17: Software Features

    • Pump and sample manager design enhancements, to minimize dispersion and reduce cycle time • Flexible column management options to support different column lengths and automated switching of up to 6 columns in independent temperature zones • An optional sample organizer to expand sample capacity 1.2.1 Software features 1.2.1.1 Quantum Synchronization Introducing a low-pressure sample into the high-pressure fluid stream during injection causes a...

  • Page 18: System Components

    Use the Auto•Blend Plus feature to create and store buffer systems in a solvent catalog that all users of an ACQUITY quaternary solvent manager can share. To prepare and adjust chromatographic mobile phases, you add acid, base, salt or organic solvent, and water to the solvent reservoirs.
  • Page 19 The system includes a solvent manager, sample manager, column heater, detector (tunable ultraviolet, photodiode array, evaporative light scattering, fluorescent, conductivity, refractive index, or mass spectrometry), and an ACQUITY UPLC column. Waters Empower chromatography software, UNIFI, or MassLynx mass spectrometry software controls the system.

  • Page 20: Quaternary Solvent Manager

    1.3.1 Quaternary solvent manager The quaternary solvent manager (QSM) is a low-pressure mixing, high-pressure pump. It provides steady (pulse-free) solvent flow at analytical flow rates to 1 mL/min at 103,421 kPa (1034 bar, 15,000 psi) and to 2.2 mL/min, at reduced pressures, to 53779 kPa (537 bar, 7800 psi).

  • Page 21: Column Heater

    1.3.4 Column manager (optional) The ACQUITY UPLC H-Class column manager is an option for helping to ensure precise, reproducible separations. The column manager can regulate the temperature of columns from 4 to 90 °C. Its troughs can accommodate columns of up to 4.6-mm I.D. and up to 150-mm length, depending on the configuration.

  • Page 22: Sample Organizer (Optional)

    system with a column manager base unit and two column heater/cooler modules can accommodate as many as six columns (two columns in the base unit). 1.3.4.1 Active solvent conditioning HPLC and UPLC applications benefit from pre-column, mobile-phase heating to improve chromatographic separations.

  • Page 23: Local Console Controller (Optional)

    1.3.9.1 eCord technology ACQUITY UPLC columns include an eCord column chip that tracks the usage history of the column. The eCord column chip interacts with the system software, recording information for as many as 50 sample queues run on the column.

  • Page 24: For Additional Information

    • ACQUITY UPLC Sample Manager - Flow Through Needle Overview and Maintenance Guide • ACQUITY UPLC Column Heater-Active Overview and Maintenance Guide • ACQUITY UPLC Column Manager - Active and Column Manager - Auxiliary Overview and Maintenance Guide • ACQUITY UPLC 30-cm Column Heater-Active Overview and Maintenance Guide •...
  • Page 25 ACQUITY UPLC online community, where you can do these things: • Share information with and ask questions of ACQUITY UPLC experts and scientists • Access ACQUITY UPLC publications and user experiences from around the globe •...

  • Page 26: Performance Optimization

    When performing fast UPLC analyses, a peak of interest can be less than 0.5 seconds in width. Waters recommends a sampling rate that will generate between 25 and 50 points across the narrowest integrated peak in the separation in order to ensure repeatable quantification and while maximizing sensitivity.

  • Page 27: Follow These General Recommendations When Performing A Uplc Analysis

    2.1.1 Follow these general recommendations when performing a UPLC analysis Select appropriate solutions • Use high-quality solvents, buffers, and additives (HPLC or MS grade). • Keep concentrated stock solutions, to use when preparing working solutions. • Start gradients that include an organic component (0.1%, for example) to provide more consistent and predictable gradient formation than when you start with 0% organic.

  • Page 28: Dispersion

    An ACQUITY UPLC H-Class system typically exhibits a bandspread between 10 and 12 µL, depending on system configuration. An Alliance HPLC system can exhibit a bandspread between 35 µL and 50 µL.

  • Page 29: Cycle Time (Between Injections)

    (downstream of the "intelligent" intake valves) can leak solvent but do not introduce air. To prevent leaks, follow Waters’ recommendations for the proper tightening of system fittings. Note specifically that different techniques apply to re-tightening fittings versus installing them for the first time.

  • Page 30: Installation Recommendations For Fittings

    2.5.1 Installation recommendations for fittings Two types of fittings are used within the system: PEEK (polymer-based) fittings and tubing and SST (stainless steel) tubing and fittings. When connecting tubing, heed the following recommendations for installing and tightening fittings. Warning: Observe Good Laboratory Practice (GLP) at all times, particularly when working with hazardous materials.
  • Page 31 To assemble the new fittings: Insert the end of a tube into the hexagonal end of the compression screw. Insert the tube into the larger end of the ferrule. Insert the tube into the fitting body. Rotate the compression screw, clockwise, into the fitting body until the screw is finger-tight. Using a permanent marker, mark the compression screw at the 12 o’clock position.
  • Page 32 Compression screw 2-piece ferrule Tighten the fitting finger-tight. 2.5.1.4 Long 1/4-28 fitting with flangeless ferrule and stainless steel lock ring installed on 1/8-inch OD tubing First use or re-installed Compression screw Lock ring Ferrule End of lock ring with larger inside diameter (ID) Tighten the fitting finger-tight.
  • Page 33 Compression screw Lock ring Ferrule End of lock ring with larger inside diameter (ID) Tighten the fitting finger-tight. 2.5.1.6 5/16-24 fitting with filter and stainless steel lock ring First use or re-installed Compression screw Lock ring Ferrule and filter End of lock ring with larger inside diameter (ID) Tighten the fitting finger-tight.
  • Page 34 Long flats Compression screw 2-piece stainless-steel ferrule Tighten the fitting finger-tight plus an additional 3/4-turn using a 1/4-inch open-end wrench. For detailed instructions about assembling new fittings, see Assembling new fittings. Tip: To prevent band spreading, ensure that the tubing is fully bottomed in the fitting before tightening the compression screw.
  • Page 35 Re-installed tightening 2.5.1.8 Stainless steel (gold-plated) fitting with short flats and 2-piece stainless-steel ferrule First use Short flats Compression screw 2-piece stainless steel ferrule Tighten the fitting finger-tight plus an additional 3/4-turn using a 1/4-inch open-end wrench. For detailed instructions about assembling new fittings, see Assembling new fittings.
  • Page 36 Re-installed Short flats Compression screw 2-piece stainless steel ferrule Tighten the fitting finger-tight plus as much as an additional 1/6-turn using a 1/4-inch open-end wrench. Re-installed tightening 2.5.1.9 PEEK fitting with PEEK ferrule and stainless steel lock ring First use or re-installed Compression screw Lock ring Ferrule...
  • Page 37 2.5.1.10 One-piece PEEK fitting First use or re-installed Compression screw Ferrule Tighten the fitting finger-tight. 2.5.1.11 Gold-plated compression screw with collet First use Hole for inserting the collet and compression-screw multi-tool Compression screw Collet PEEK ferrule To tighten this fitting for first use: Finger-tighten the fitting.
  • Page 38 Placement of collet and compression-screw multi-tool and 5/16-inch open-end wrench Collet and compression-screw multi-tool Column or column in-line filter 5/16-inch open-end wrench Tip: To prevent band spreading, ensure that the tubing bottoms in its fitting before you tighten the compression screw. First use tightening Re-installed August 8, 2016, 715005049 Rev.
  • Page 39 Hole for collet and compression-screw multi-tool Compression screw Collet PEEK ferrule To tighten a re-installed fitting: Insert the tool between the collet and compression screw and pry the collet until it loosens from the inside of the fitting. Re-installed Hole for collet and compression-screw multi-tool Collet and compression-screw multi-tool Collet PEEK ferrule...
  • Page 40 Placement of collet and compression-screw multi-tool and 5/16-inch open-end wrench Collet and compression-screw multi-tool Column or column in-line filter 5/16-inch open-end wrench Tip: When reinstalling the fitting, • examine the collet and ferrule for damage, and replace them, if necessary; •...

  • Page 41: Developing Methods

    2.7.1 Particulates Waters recommends filtering all samples with particulates through a 0.2 µm sample filter or installing a column pre-filter. The small column frit size (0.2 µm) can become blocked more easily than larger HPLC column frits (2.0 µm). As a result, particle-free mobile phase solvents and August 8, 2016, 715005049 Rev.

  • Page 42: Matching Sample Diluents

    HPLC column, whereby selectivity and resolution are maintained. Using the ACQUITY UPLC columns calculator, it is then scaled to UPLC and optimized for the shortest analysis time at equal peak capacity. The run time decreased by 46 minutes.
  • Page 43 Using the Waters ACQUITY UPLC Columns Calculator ensures the best results for transferring the LC method from HPLC to UPLC or UPLC to HPLC.

  • Page 44: Columns Calculator

    • For a target system, with a smaller volume, use an isocratic hold to account for the dwell volume differences. • Active preheating is the default configuration for the ACQUITY UPLC H-Class system. An optional, passive, column stabilizer is available for existing chromatographic methods not suitable for active preheating.
  • Page 45 L/dp of the existing method column and the target column. Because the dwell volumes of the ACQUITY UPLC H-Class system are far smaller than those of a conventional HPLC system, often a gradient hold is required.

  • Page 46: System Preparation

    System preparation Before proceeding, ensure that all of the procedures that explain how to prepare the system modules for operation were performed as specified in the modules' overview and maintenance guides. 3.1 Powering-on the system To power-on the system, you must power-on the system workstation, system modules, and the chromatography software.

  • Page 47: Opening The Console

    Requirement: If this is the first time you are using this system, you must define a new system. For instructions, see the online Help. Open the control panels and console. See also: Monitoring from control panels Opening the console Prime the system. See also: Priming the system 3.2 Opening the console...

  • Page 48: To Open The Console From Unifi Software

    3.2.3 To open the console from UNIFI software From the UNIFI Portal, click the My Work tab. From the My Work tab, select Instrument Systems, and then double-click on the device that you want to monitor. Alternative: Launch the System Console from the System Control Panel menu. Result: A control panel for the selected device appears.
  • Page 49 Alternative: Firmware upload is in progress. Steady red A failure is preventing operation. Cycle power to the sample manager. If the LED remains red, report the problem to Waters Technical Support. Alternative: Firmware upload is complete. 3.4.2.2 Flow LED Flow status is indicated by an LED on the solvent manager’s front panel. The flow LED is on the right-hand side of the power LED.

  • Page 50: Monitoring From Control Panels

    Steady red A failure is preventing the detector from operating. If, after you cycle power to the detector, the LED remains red, report the problem to Waters Technical Service. 3.5 Monitoring from control panels You can monitor the sample manager, solvent manager, column module, and detector from control panels, which you access via the chromatography data system.

  • Page 51: Column Manager Control Panel

    • When Empower software controls the system, the control panels appear at the bottom of the Run Samples window. • When MassLynx software controls the system, the control panels appear on the Additional Status tab of the Inlet Editor window. •...

  • Page 52: Sample Manager Control Panel

    Table 3–4: Additional functions in the column manager's control panel Control panel function Description Reset module Resets the column manager after an error condition. Help Displays the console online Help. 3.5.2 Sample manager control panel The control panel of the sample manager-flow through needle (SM-FTN) displays the current temperatures of the sample compartment and column heater, their set points, and the selected column.

  • Page 53: Solvent Manager Control Panel

    You can access these additional functions by right-clicking anywhere in the sample manager control panel. Table 3–5: Additional functions in the sample manager control panel Control panel function Description Prime Displays the Prime dialog box. Wash needle Displays the Wash Needle dialog box. Launch Console Launches the console software.

  • Page 54: Els Control Panel

    You can access these additional functions by right-clicking anywhere in the solvent manager control panel. Table 3–6: Additional functions in solvent manager the control panel Control panel function Description Start up system Brings the system to operational conditions after an extended idle period or when switching to different solvents.

  • Page 55: Flr Control Panel

    Lamp icon – When clicked, ignites or extinguishes the lamp. If the icon is green, the lamp is ignited. If the icon is red, the lamp is extinguished. Current nebulizer temperature – Displays the current nebulizer temperature. Current nebulizer gas pressure – Displays the current nebulizer gas pressure. Photomultiplier tube gain factor –...

  • Page 56: Pda Control Panel

    Lamp LED – Mirrors the lamp status LED on the front panel of the detector, unless communications with the detector are lost. Emission units or energy units – Displays the emission units or energy units. Lamp icon – When clicked, ignites or extinguishes the lamp. If the icon is green, the lamp is ignited.

  • Page 57: Ri Control Panel

    Figure 3–6: PDA detector control panel Lamp LED – Mirrors the lamp status LED on the front panel of the detector, unless communications with the detector are interrupted. Lamp icon – When clicked, ignites or extinguishes the lamp. If the icon is green, the lamp is ignited.

  • Page 58: Tuv Control Panel

    Figure 3–7: RI detector control panel Run status LED – Mirrors the run status LED on the front panel of the detector, unless communications with the detector are interrupted. Recycle indicator – When clicked, the recycle valve changes positions to avoid wasting solvent when equilibrating the detector.

  • Page 59: 2432 Conductivity Control Panel

    You can edit detector parameters when the system is idle by clicking the underlined value. You cannot edit these values while the system is running samples. Figure 3–8: TUV detector control panel Lamp LED – Mirrors the lamp status LED on the front panel of the detector, unless communications with the detector are interrupted.

  • Page 60: Starting Up The System

    Figure 3–9: 2432 conductivity detector control panel Autozero button – Eliminates the eluent's contribution to conductivity. Cell temperature set point – Displays the set point for the flow cell temperature. Current cell temperature – Displays the current flow cell temperature. Peak polarity –...
  • Page 61 To start up the system: From the system view of the console, click Control > Start up system. Alternative: Right-click in the control panel and click Start up system. On the Prime Solvents tab, click the solvent manager sub-tab and if necessary, change the value in the Duration of Prime field.
  • Page 62 Table 3–13: Equilibrate to Method table values (continued) System startup Default Allowed values parameters Composition of A, B, A, 100% A; 0 to 100% C, and D (sum must B, C, D, 0% B; 0 to 100% be 100%) C; 0 to 100% D;...

  • Page 63: System Maintenance

    4.1 Contacting Waters Technical Service If you are located in the USA or Canada, report malfunctions or other problems to Waters Technical Service (800-252-4752). From elsewhere, phone the Waters corporate headquarters in Milford, Massachusetts (USA), or contact your local Waters subsidiary. The Waters Web site includes phone numbers and e-mail addresses for Waters locations worldwide.

  • Page 64: Maintenance Procedures And Frequency

    Connections INSIGHT is an intelligent device management (IDM) Web service that enables Waters to provide proactive service and support for a system. To use Connections INSIGHT, you must install its service agent software on a MassLynx or Empower workstation. In a client/server system, you must also install the service agent on the computer that controls the system.

  • Page 65: To Submit A Connections Insight Request

    Connections INSIGHT iAssist service level. These sources, available on the Waters’ Web site, provide more information about Connections INSIGHT and Connections INSIGHT iAssist: • Connections INSIGHT Quick Start Guide • Connections INSIGHT User's Guide 4.4.1 To submit a Connections INSIGHT request:...

  • Page 66: External Connections

    Installation recommendations for fittings. Note: A Waters Technical Service representative unpacks and installs the system components. Warning: To avoid spinal and muscular injury, do not attempt to lift a system module without assistance. If you must transport a system component, or remove it from service, request recommended cleaning, flushing, and packaging procedures from Waters Technical Service.

  • Page 67: External Cable Connections

    5.2 External cable connections Figure 5–1: System rear-panel cable connections Detector Column heater Sample manager (SM-FTN) Solvent manager (QSM) Ethernet switch Workstation Interconnect (D-sub) cables Ethernet cables August 8, 2016, 715005049 Rev. C Page 67...

  • Page 68: Plumbing Connections

    5.3 Plumbing connections Figure 5–2: System plumbing connections Detector Column heater Sample manager (SM-FTN) Solvent manager (QSM) Stainless steel tubing PEEK tubing August 8, 2016, 715005049 Rev. C Page 68...

  • Page 69: Waste-Tubing Connections

    5.4 Waste-tubing connections Figure 5–3: System waste-tubing connections Solvent bottle tray Detector August 8, 2016, 715005049 Rev. C Page 69...

  • Page 70: Electricity Source

    To avoid electrical shock, observe these precautions: • Use SVT-type power cord in the United States and HAR-type power cord, or better, in Europe. For requirements elsewhere, contact your local Waters distributor. • Inspect the power cord for damage, and replace it, if necessary.

  • Page 71: Connecting To A Cart's Electricity Source

    To avoid electrical shock, observe these precautions: • Use SVT-type power cord in the United States and HAR-type power cord, or better, in Europe. For requirements elsewhere, contact your local Waters distributor. • Inspect the power cord for damage, and replace it, if necessary.
  • Page 72 Figure 5–4: Inserting connector into connector port Connector port Connector Using the flat-blade screwdriver, attach the positive and negative leads of the signal cable to the connector. Figure 5–5: Positive and negative lead connections Screw Connector Signal cable Fit the grounding cable's fork terminal on the rear-panel grounding stud, and secure the terminal using the locking nut.

  • Page 73: Connecting To A Column Module

    Figure 5–6: Grounding cable fork terminal on grounding stud Fork terminal Locking nut Grounding stud 5.7 Connecting to a column module The following column modules are compatible with the ACQUITY UPLC H-Class system: • Column heater • Column heater 30 cm • Column heater/cooler • Column manager •...
  • Page 74 To connect the column module: Notice: To avoid damaging the electronic components of the sample manager and the column heater or column heater/cooler, always power-off the sample manager and column heater/cooler before connecting or disconnecting the interconnect cable. Make sure the sample manager and the column module are powered-off. Connect the interconnect cable to the High Density (HD) port on the rear of the column module.

  • Page 75: A Safety Advisories

    Heed all warnings when you install, repair, or operate any Waters instrument or device. Waters accepts no liability in cases of injury or property damage resulting from the failure of individuals to comply with any safety precaution when installing, repairing, or operating any of its instruments or devices.

  • Page 76: Specific Warnings

    (Risk of explosion.) A.1.1 Specific warnings A.1.1.1 Burst warning This warning applies to Waters instruments and devices fitted with nonmetallic tubing. Warning: To avoid injury from bursting, nonmetallic tubing, heed these precautions when working in the vicinity of such tubing when it is pressurized: •...

  • Page 77: Notices

    A.1.1.2 Biohazard warning The following warning applies to Waters instruments and devices that can process material containing biohazards, which are substances that contain biological agents capable of producing harmful effects in humans. Warning: To avoid infection with potentially infectious, human-sourced products, inactivated microorganisms, and other biological materials, assume that all biological fluids that you handle are infectious.

  • Page 78: Required Protection

    Use eye protection when performing this procedure. Requirement: Wear clean, chemical-resistant, powder-free gloves when performing this procedure. A.5 Warnings that apply to all Waters instruments and devices When operating this device, follow standard quality-control procedures and the equipment guidelines in this section.
  • Page 79 警告： 未經有關法規認證部門允許對本設備進行的改變或修改,可能會使使用者喪失操作 該設備的權利。 警告： 未经有关法规认证部门明确允许对本设备进行的改变或改装，可能会使使用者丧 失操作该设备的合法性。 경고: 규정 준수를 책임지는 당사자의 명백한 승인 없이 이 장치를 개조 또는 변경할 경우, 이 장치를 운용할 수 있는 사용자 권한의 효력을 상실할 수 있습니다. 警告: 規制機関から明確な承認を受けずに本装置の変更や改造を行うと、本装置のユーザーとして の承認が無効になる可能性があります。 Warning: Use caution when working with any polymer tubing under pressure: •...
  • Page 80 Warnung: Bei der Arbeit mit Polymerschläuchen unter Druck ist besondere Vorsicht angebracht: • In der Nähe von unter Druck stehenden Polymerschläuchen stets Schutzbrille tragen. • Alle offenen Flammen in der Nähe löschen. • Keine Schläuche verwenden, die stark geknickt oder überbeansprucht sind. •...
  • Page 81 圧力のかかったポリマーチューブを扱うときは、注意してください。 • 加圧されたポリマーチューブの付近では、必ず保護メガネを着用してください。 • 近くにある火を消してください。 • 著しく変形した、または折れ曲がったチューブは使用しないでください。 • 非金属チューブには、テトラヒドロフラン(THF)や高濃度の硝酸または硫酸などを流さないでくださ い。 • 塩化メチレンやジメチルスルホキシドは、非金属チューブの膨張を引き起こす場合があり、その場 合、チューブは極めて低い圧力で破裂します。 This warning applies to Waters instruments fitted with nonmetallic tubing. This warning applies to instruments operated with flammable solvents. August 8, 2016, 715005049 Rev. C Page 81...

  • Page 82: Warnings That Address The Replacing Of Fuses

    Warning: The user shall be made aware that if the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. Avertissement : L’utilisateur doit être informé que si le matériel est utilisé d’une façon non spécifiée par le fabricant, la protection assurée par le matériel risque d’être défectueuses.
  • Page 83 Avertissement : pour éviter tout risque d'incendie, remplacez toujours les fusibles par d'autres du type et de la puissance indiqués sur le panneau à proximité du couvercle de la boite à fusible de l'instrument. Warnung: Zum Schutz gegen Feuer die Sicherungen nur mit Sicherungen ersetzen, deren Typ und Nennwert auf den Tafeln neben den Sicherungsabdeckungen des Geräts gedruckt sind.

  • Page 84: Electrical Symbols

    警告： 為了避免火災，更換保險絲時，應使用「維護步驟」章節中「更換保險絲」所指 定之相同類型與規格的保險絲。 警告： 为了避免火灾，应更换“维护步骤”一章的“更换保险丝”一节中介绍的相同类 型和规格的保险丝。 경고: 화재의 위험을 막으려면 유지관리 절차 단원의 “퓨즈 교체” 절에 설명된 것과 동 일한 타입 및 정격의 제품으로 퓨즈를 교체하십시오. 警告: 火災予防のために、ヒューズ交換ではメンテナンス項目の「ヒューズの交換」に記載されているタ イプおよび定格のヒューズをご使用ください。 A.7 Electrical symbols The following electrical symbols and their associated statements can appear in instrument manuals and on an instrument’s front or rear panels.

  • Page 85: Handling Symbols

    A.8 Handling symbols The following handling symbols and their associated statements can appear on labels affixed to the packaging in which instruments, devices, and component parts are shipped. Symbol Description Keep upright! Keep dry! Fragile! Use no hooks! Upper limit of temperature Lower limit of temperature Temperature limitation August 8, 2016, 715005049 Rev.

  • Page 86: B Solvent Considerations

    • ACQUITY UPLC FLR detector • 2432 Conductivity detector B.1 Solvent recommendations The system is designed for reversed-phase chromatography and ACQUITY UPLC BEH column chemistries. Waters evaluated the system’s reliability using traditional reversed-phase solvents. Notes: • When implementing passive check valves on ACQUITY systems, Waters recommends that you use MS Grade solvents.

  • Page 87: Recommended Solvents

    Store mobile phases in borosilicate glass reservoirs type 1, class A type 3.3 . Use high-quality, brown-tinted glassware to inhibit microbial growth. Use aluminum foil or Waters caps to cover the reservoirs. August 8, 2016, 715005049 Rev. C Page 87...

  • Page 88: Water

    Visit www.waters.com. B.5 Other solvents You can use the following solvents. Note, however, that these solvents can shorten instrument life. If you routinely use the solvents on this list, Waters recommends you install the Hexane/THF Compatibility Kit. • Tetrahydrofuran (THF) •...

  • Page 89: Hexane/Thf Compatibility Kit

    The ACQUITY UPLC System Hexane/THF Compatibility Kit (contact Waters for part number) can be installed in ACQUITY UPLC systems with closed waste management. It is designed for users that need to run their systems with hexane or THF at high concentrations and high pressure and is recommended for many ELS detector-based applications where THF is used in the mobile phase, at high concentrations.

  • Page 90: Cleaning Agents

    Do not use buffers as needle wash. B.9 Cleaning agents Recommendation: See the cleaning procedures in Controlling Contamination in Ultra Performance LC/MS and HPLC/MS Systems (part number 715001307) on the Waters Web site. Visit www.waters.com. • Phosphoric acid (≤30%) • Sodium hydroxide (≤1M) •...

  • Page 91: Quaternary Solvent Manager Recommendations

    • THF, hexane, ethyl acetate, and acetone can be used as the mobile-phase in ACQUITY UPLC H-Class systems. However, as with many nonaqueous solvents, they can shorten system and instrument life compared with equipment running typical reversed-phase solvents.

  • Page 92: Sample Manager - Flow Through Needle Recommendations

    B.13 Sample manager - flow through needle recommendations • Do not use concentrations of THF or hexane greater than 10% as purge solvent. • Typical organic sample diluents such as dimethylsolfoxide (DMSO) and dimethylformamide (DMF) are supported. Notice: • Do not leave buffers stored in the system. •...
  • Page 93 Table B–1: Properties of common solvents (continued) Solvent Vapor pressure mm Hg (Torr) Boiling point Flash point (°C) (°C) N, N-Dimethylformamide 2.7 at 20 °C 153.0 Dimethyl sulfoxide 0.6 at 25 °C 189.0 1, 4-Dioxane 29 at 20 °C 101.32 Ethyl acetate 73 at 20 °C 77.11...

  • Page 94: Solvent Miscibility

    B.14.1 Solvent miscibility Before you change solvents, refer to the table below to determine solvent miscibility. Be aware of these effects: • Changes involving two miscible solvents can be made directly. Changes involving two solvents that are not totally miscible (for example, from chloroform to water) require an intermediate solvent like n-propanol.

  • Page 95: Solvent Stabilizers

    To predict the miscibility of two liquids, subtract the smaller M-number value from the larger M- number value. • If the difference between two M-numbers is 15 or less, the two liquids are miscible, in all proportions, at 15 °C. •...

  • Page 96: Wavelength Selection

    B.14.4 Wavelength selection The tables in this section provide UV cutoff values for these items: • Common solvents • Common mixed mobile phases B.14.4.1 UV cutoffs for common solvents The table below shows the UV cutoff (the wavelength at which the absorbance of the solvent equals 1 AU) for some common chromatographic solvents.
  • Page 97 TRIS HCl, 20 mM, pH 8.0 Triton X-100, 0.1% Waters PIC Reagent A, 1 vial/liter Waters PIC Reagent B-6, 1 vial/liter Waters PIC Reagent B-6, low UV, 1 vial/liter Waters PIC Reagent D-4, 1 vial/liter August 8, 2016, 715005049 Rev. C Page 97...
  • Page 98 B.14.4.3 Mobile phase absorbance This section lists the absorbances at several wavelengths for frequently used mobile phases. Choose the mobile phase carefully to reduce baseline noise. The best mobile phase for your application is one that is transparent at the chosen detection wavelengths. With such a mobile phase, ensure that any absorbance is caused only by the sample.
  • Page 99 — — — Tris HCl, 20 mM, pH 8.0 1.80 1.90 1.11 0.43 0.13 <0.01 — — — — Waters PIC reagents PIC A, 1 vial/L 0.67 0.29 0.13 0.05 0.03 0.02 0.02 0.02 0.02 <0.01 PIC B6, 1 vial/L 2.46...
  • Page 100 Table B–5: Mobile phase absorbance measured against air or water (continued) Absorbance (AU) at specified wavelength (nm) PIC D4, 1 vial/L 0.03 0.03 0.03 0.03 0.02 0.02 0.02 0.02 0.02 0.01 BRI J 35, 1% 0.06 0.03 0.02 0.02 0.02 0.01 <0.01 —...

  • Page 101: C Specifications

    Specifications The reproducibility of the specifications presented in this document depends on the conditions in individual laboratories. Contact the Waters Technical Service organization for additional information about specifications. C.1 System specifications See also: For individual module physical, environmental, and input/out specifications consult the module's overview and maintenance guide.

  • Page 102: Instrument Control Specifications

    Test conditions: • System: ACQUITY UPLC H-Class quaternary solvent manager (QSM), ACQUITY UPLC H-Class sample manager with flow through needle (SM-FTN), ACQUITY UPLC H-Class column heater with active pre-heater (CH-A), and ACQUITY UPLC TUV detector •...

  • Page 103: Environmental Specifications

    Item Specification Connections INSIGHT Provides real-time monitoring and automatic notification of instrument performance and diagnostic information Local control ACQUITY UPLC Local Console Controller (LCC) C.1.2 Environmental specifications Attribute Specification Acoustic noise, system <65 dBA Ambient operating temperature 4.0 to 40.0 °C...

  • Page 104: Physical Specifications

    Attribute Specification Power consumption QSM: 360 VA SM-FTN: 400 VA Column manager with active pre-heater (CM-A): 400 VA Sample organizer: 540 VA a. Protection Class I – The insulating scheme used in the instrument to protect from electrical shock. Class I identifies a single level of insulation between live parts (wires) and exposed conductive parts (metal panels), in which the exposed conductive parts are connected to a grounding system.

  • Page 105: Qsm And Bioqsm Performance Specifications

    • Gradient conditions: 1.0 to 33% B in 10 min; time average window, 10 s. Noise range 4.00 to 6.00 min • Column: ACQUITY UPLC BEH C , 1.7 µm, 2.1 × 50 mm • Detector: ACQUITY TUV, 214 nm wavelength, 10 Hz sampling rate August 8, 2016, 715005049 Rev. C Page 105...
  • Page 106 • Flow rate: 0.5 mL/min • Sample mix: alkylphenone mix (5.0 µL injection volume) • Column: ACQUITY UPLC BEH C , 1.7 µm, 2.1 × 50 mm • Column temperature: 35 °C ±0.3 °C • Detector: UV, 254 nm wavelength...

  • Page 107: Sm-Ftn And Biosm-Ftn Performance Specifications

    • Needle volume: 1 to 70% • Sample mix: caffeine 0.03 mg/mL (0.2 to 10.0 µL, with 15 µL needle installed, no extension loop) • Column: ACQUITY UPLC BEH C , 1.7 µm, 2.1 × 50 mm • Detector: UV, 273-nm wavelength •...
  • Page 108 Waters website. Maximum sample 768 in two, 384-well Waters-certified plates, or 96 in 2-mL vial holders. capacity Four additional positions for dilution functions. For more information, see the Waters Sample Vials and Accessories Brochure, part number 720001818 or visit the...
  • Page 109 • Solvent B: 100% acetonitrile • Weak wash: 10:90 acetonitrile/water • Strong wash: 10:90 acetonitrile/water • Column: ACQUITY UPLC BEH C 1.7 mm, 2.1 × 50 mm • Mobile phase: 90% solvent A:10% solvent B • Flow rate: 0.6 mL/min •...
  • Page 110 (challenge) in 10:90 acetonitrile/water +0.1% formic acid, compared with blanks of 10:90 acetonitrile/water +0.1% formic acid • Injection volume: 5 µL • Column: ACQUITY UPLC BEH C 1.7 µm, 2.1 × 50 mm • Column temperature: 40 °C • Sample temperature: 10 °C •...
  • Page 111 Figure C–1: Recommended temperature sensor locations TS Temperature sensor Figure C–2: Minimum sample compartment temperature specifications Maximum compartment temperature +4 °C Minimum sustainable compartment set point temperature -2 °C Minimum compartment temperature Ambient temperature (°C) August 8, 2016, 715005049 Rev. C Page 111...

  • Page 112: Ch-A Performance Specifications

    C.2.3 CH-A performance specifications Item Specification Settable temperature 20.0 to 90.0 °C, in 0.1 °C increments range Controllable temperature (Ambient +5 °C) to 90.0 °C range Tested to ±0.5 °C between 20 and 50 °C (±1.0 °C for remaining Temperature accuracy range) Test qualification: The temperature measured by a NIST-traceable probe located next to the measurement sensor must fall within this...

  • Page 113: 30-Cm Chc Performance Specifications

    Item Specification Leak control Compartment drip tray with leak sensor installed under compartment. Single exit drain manages leaks to waste. Door open to heater cutoff 1-minute maximum delay Column tracking eCord technology column information management tracks and archives column usage history. a.
  • Page 114 Item Specification Temperature Tested to ±0.5 °C Test conditions: accuracy • Door closed • No column installed • No flow • Measurement taken with traceable, external temperature measurement device • Measurement taken after 1 hour of thermal equilibration at set point •...

  • Page 115: Sample Organizer Performance Specifications

    Item Specification Temperature stability Tested to ±0.3 °C Test conditions: • Door closed • No column installed • No flow • Measurement taken with traceable, external temperature measurement device • Measurement taken for 1 hour after thermal equilibration at set point •...
  • Page 116 Item Specification Maximum sample plate 53 mm height (includes vials, caps, and cap mats) Sample compartment Between 4 and 40 °C, in increments of 0.1 °C, with a tolerance range temperature range of between -2 and +4 °C • At a setpoint of 4 °C with ambient temperature <23 °C and humidity <80%, maintains a sample temperature of 2 to 8 °C.

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