Quality Control; Cell Location Test For Peripheral Blood - CellaVision DM1200 User Manual

Quality control

3 Quality control

3.1 Cell location test for peripheral blood

The cell location test verifies that the quality of the slide preparation process is
good enough to allow the system to locate the cells needed for the analysis. It
also verifies the system's ability to locate cells.
When you run a cell location test, the system will try to locate a monolayer on a
peripheral blood slide and will then try to locate 200 nucleated cells (that is,
WBCs and NRBCs) in that monolayer. It will then show you overview images,
with the located cells marked.
You can verify that the system has located all nucleated cells, by examining the
overview images and counting any cells that have not been located by the
system. The system will then calculate how many percent of the nucleated cells
that have been located.
To prepare and process a cell location slide
1. Use a freshly stained slide from a blood sample with a WBC count within the
normal range:
• A WBC count above 7 × 10
• If the system cannot locate at least 100 nucleated cells on the slide, the
• The percentage of non-nucleated cells must not exceed 50% of the total
2. Put a barcode with an order ID beginning with "QC" on the slide.
Barcode labels with "QC" order ID are available from your local vendor.
3. Put the slide in a magazine, put the magazine in the system and process the
slide.
4. When the slide has been processed, examine the cell location slide as
described later in this section.
18
CellaVision
Important!
Run a cell location test at least once a day. Also run a cell location test
after any changes in the staining procedure or the staining solutions.
This will help to prevent misleading results caused by the system failing
to locate cells.
For a system with a high work load, it is recommended to run cell
location tests more often.
concentration, the test will take longer time).
test result will be discarded.
number of objects on the slide.
Non-nucleated cells are all objects that the system doesn't preclassify as
being a WBC or an NRBC, for example smudge cells.
PM-10829-01 2015-07-10
® DM1200
9
/L is recommended (with a lower
User's Manual