Chapter 7 Sample Preparation And Measurement - THORLABS OTKB/M User Manual

Modular Optical Tweezers Chapter 7: Sample Preparation and Measurement
Chapter 7 Sample Preparation and Measurement
For initial testing we recommend 1 μm or 2 μm silica beads. The sample solution loaded into the channel using a
microscopy slide with built-in channel (offered via our optical trapping accessories kit, Thorlabs item number
OTKBTK, sold separately), or you can build a simple channel by placing double sided tape on a standard slide, and
adding a cover glass on top. Liquid can be pipetted in-between. The two open sides can be sealed off with nail
polish, to prevent the sample from drying out. Place the slide onto the sample holder and carefully place the slide
between objective and condenser. Make sure to either use immersion oil on the bottom of the slide or to apply it to
the objective before trying to image the sample.
Figure 11 Beads are diluted using a pipette and placed between strips of double sided tape.
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