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Summary of Contents for Hoefer SG15
- Page 1 Hoefer SG15, SG30, SG50 and SG100 Gradient makers SG100-IM/Rev.C0/08-12...
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Page 2: Table Of Contents
Page finder 1. Introduction: Hoefer SG15, SG30, SG50, and SG100 gradient makers .......1 2. Generating linear gradients .........2 Pouring gradients from the top ......4 Pouring gradients from the bottom ......5 3. Care and maintenance ........6 4. Ordering information ..........6 •... -
Page 3: Introduction: Hoefer Sg15, Sg30, Sg50, And Sg100 Gradient Makers
The gradient makers have a white Luer outlet fitting that can take either tubing with female reservoir (black) Fig 1. Hoefer SG gradient maker. chamber mixing (front) chamber Included but not shown:... -
Page 4: Generating Linear Gradients
For the most consistent delivery of gradients, a peristaltic pump is recommended. Table 1. Operating ranges for linear gradients model max total vol min vol/chamber max stir bar length (ml) (ml) (mm) SG15 SG30 SG50 SG100 •... - Page 5 Make sure all parts are clean and liquid flows freely through all channels, stopcocks and tubing. Add a magnetic stir bar of the appropriate dimensions to the mixing chamber (Table 1) and place the unit on a magnetic stirrer. If volumes will be less than half the capacity of the unit, an identical stir bar should be placed in the reservoir chamber as well to balance the displacement and prevent backflow into the reser-...
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Page 6: Pouring Gradients From The Top
If it is important that no bubbles disturb the gradient, watch the delivery carefully and as soon as the last of the solution has entered the pump head, stop the pump and remove the tubing from the receiving container. Flush and rinse all parts thoroughly with distilled water after use. -
Page 7: Pouring Gradients From The Bottom
Pouring gradients from the bottom This technique is commonly used in filling multiple gel casting chambers. Proceed as described on page 3, putting dense (final or bottom) solution in the reservoir chamber (step 3) and light solution in the mixing chamber (step 5). Connect the delivery tubing to the bottom inlet of a gel casting unit or to a cannula long enough to reach the bottom of the receiving container. -
Page 8: Care And Maintenance
SG50 50 ml total volume SG100 gradient maker, SG100 100 ml total volume White Luer outlet fitting, (4 mm) SG100-6 SG15, SG30, SG50, SG100 Stopcock, standard size, SG100-1 for SG15, SG30, SG50 Long stopcock, for SG100, SG100-9 connector stopcock Short stopcock, for SG100,... - Page 9 Hoefer, Inc. 84 October Hill Road Holliston, MA 01746 Toll Free: 1-800-227-4750 Phone: 1-508-893-8999 Fax: 1-508-893-0176 E-mail: support@hoeferinc.com Web: www.hoeferinc.com Hoefer is a registered trademark of Hoefer, Inc. © 2012 Hoefer, Inc. — All rights reserved. Printed in the USA. •...
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