Advertisement
Quick Links
Advertisement
Related Manuals for Hoefer SG500
Summary of Contents for Hoefer SG500
- Page 1 Hoefer SG500 Gradient maker SG500-IM/Rev.E0/08-12...
-
Page 2: Table Of Contents
Page finder SG500 instructions ..........1 1. Generating linear gradients .........2 Pouring gradients from the top ......4 Pouring gradients from the bottom ......5 2. Generating convex and concave exponential gradients ............6 To generate an exponential gradient ....7 3. Care and maintenance ........9 4. -
Page 3: Sg500 Instructions
SG500 gradient maker is designed for producing linear gradients of aqueous solutions ranging in volume from 100–500 ml. The SG500 can be used to create convex and concave exponential gradients with the addition of a one-holed rubber stopper, a piece of rigid tubing and a piece of flexible tubing. -
Page 4: Generating Linear Gradients
1. Generating linear gradients To generate a linear gradient between two concentrations, equal volumes of solutions of the two concentrations are measured into the two chambers of the gradient maker. As solution is delivered out of the mixing chamber, an equal volume flows in from the reservoir chamber where it is rapidly diluted and mixed to unifor- mity by a magnetic stir bar. - Page 5 Carefully open the slide valve and allow just enough Note: If there is a substan- solution to flow through the connector channel to fill tial difference in densities it to the edge of the mixing chamber, then close the between the two solutions, valve.
-
Page 6: Pouring Gradients From The Top
Pouring gradients from the top Filling a container with a gradient (e.g. casting acrylamide pore gradient gels) can be done either dense solution first (“from the top”), or light solution first (“from the bottom”). To fill from the top: Proceed as described in Section 1, putting light (final or top) solution in the reservoir chamber (step 3) and dense solution in the mixing chamber (step 5). -
Page 7: Pouring Gradients From The Bottom
Pouring gradients from the bottom This technique is commonly used in filling multiple gel casting chambers. Proceed as described in Section 1, putting dense (final or bottom) solution in the reservoir chamber (step 3) and light solution in the mixing chamber (step 5). -
Page 8: Generating Convex And Concave Exponential Gradients
(as opposed to declining for linear gradients), the gradient generated will have an exponential concentration curve. The mixing volume in the SG500 unit can be held constant by sealing the top of the mixing chamber with a rubber stopper and tubing assembly (Fig 4). The... -
Page 9: To Generate An Exponential Gradient
Fig 3. Examples of exponential gradients. 100% % of gradient delivered To generate an exponential gradient: Make sure all parts are clean and liquid flows freely through all channels, stopcocks and tubing. Assemble the stopper. Select a one-hole rubber stop- per that fits securely in the top of the mixing cham- ber. - Page 10 Close the slide valve and add a portion of the final solution to the reservoir (back) chamber. Carefully open the slide valve and allow just enough solution to flow through the connector channel to fill it to the edge of the mixing chamber, then close the valve.
-
Page 11: Care And Maintenance
Do not use abrasives, acetone, pure alcohols or organic solvents to clean this unit. 4. Ordering information product qty. code no. SG500 Gradient Maker, SG500 500 ml total volume Push-pull valve for salt gradient SG500-10 Red outlet fitting, 4 mm XPO10... - Page 12 Hoefer, Inc. 84 October Hill Road Holliston, MA 01746 Toll Free: 1-800-227-4750 Phone: 1-508-893-8999 Fax: 1-508-893-0176 E-mail: support@hoeferinc.com Web: www.hoeferinc.com Hoefer is a registered trademark of Hoefer, Inc. © 2012 Hoefer, Inc. — All rights reserved. Printed in the USA.
Need help?
Do you have a question about the SG500 and is the answer not in the manual?
Questions and answers