Hoefer TE70X User Manual
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Hoefer TE70X User Manual

Semi-dry blotters, transfer unit
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TE70X Semi-dry Blotters
TE70X Semi-dry transfer unit
TE77X Semi-dry transfer unit
TE70XP Semi-dry transfer unit
TE77XP Semi-dry transfer unit
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TE70X-IM/Rev. A0/12-05

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Summary of Contents for Hoefer TE70X

  • Page 1 TE70X Semi-dry Blotters TE70X Semi-dry transfer unit TE77X Semi-dry transfer unit TE70XP Semi-dry transfer unit TE77XP Semi-dry transfer unit TE70X-IM/Rev. A0/12-05...

  • Page 3: Table Of Contents

    Waste Electrical and Electronic Equipment (WEEE) . . v 1. TE70X Semi-dry blotters: Description ...1 Unpacking ......2 Specifications .

  • Page 4: Important User Information

    Hoefer, Inc. Hoefer, Inc. shall in no event be liable for incidental or consequential damages, including without limitation, lost profits, loss of income, loss of business opportunities, loss of use and other related exposures, however caused, arising from the faulty and incorrect use of the product.
  • Page 5 Einhaltung der veröffentlichten Spezifikationen getestet wurde. Die in den Lieferbedingungen näher erläuterten Gewährleistungsansprüche gelten nur dann, wenn das Produkt gemäß den von Hoefer, Inc. geliefer- ten Anweisungen installiert und benutzt wurde. Hoefer, Inc. übernimmt keinerlei Haftung für Schäden oder Folge- schäden, einschließlich, aber nicht begrenzt auf Gewinneinbußen,...
  • Page 6 Hoefer, Inc. 953 Indiana Street San Francisco, CA 94107 USA support@hoeferinc.com Hoefer, Inc. si riserva il diritto di apportare modifiche ai dati tecnici senza preavviso. Garanzia e responsabilitá Hoefer, Inc. garantisce che prima della consegna il prodotto è stato collaudato a fondo per soddisfare i requisiti specificati. La garanzia inclusa nelle condizioni di consegna risulta valida solamente se il prodotto è...

  • Page 7: Waste Electrical And Electronic Equipment (Weee)

    Waste Electrical and Electronic Equipment (WEEE) This symbol indicates that the waste of electrical and English electronic equipment must not be disposed as unsorted municipal waste and must be collected separately. Please contact an authorized representative of the supplier for information concerning the decommissioning of your equipment.
  • Page 8 • TE70X-IMA0...

  • Page 9: Te70X Semi-Dry Blotters: Description

    SE 400. The larger TE77X transfer surface is 21 × 26 cm, suitable for transferring large format gels. The TE70X and TE77X have safety circuits built lower electrode into the instrument that limit the voltage to 30 V and the current to 0.5 A.

  • Page 10: Unpacking

    The TE70X and TE77X items arrived. If any part is missing, contact require a power supply Hoefer, Inc. Inspect all components for damage with a minimum rating of 0 – 500 mA, 0 – 30 V. that may have occurred while the unit was in transit.

  • Page 11: Specifications

    14 × 16 21 × 26 when it is: Output: • used in laboratory locations. Voltage (V) • used as delivered from Hoefer, Current (A) Inc. except for alterations Inputs: described in the user manual. Voltage (V) 100 – 240 100 –...

  • Page 12: Important Information

    • If this equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. • Only accessories and parts approved or supplied by Hoefer, Inc. may be used for operating, maintaining, and servicing this product. Informations importantes Français •...

  • Page 13: Operating Instructions

    2. Operating instructions To transfer proteins, prepare the unit, assemble the stack, and connect to a power supply if necessary. Then run the transfer for the required amount of time. Each step is described below. Prepare the unit by rinsing the electrodes with distilled water.
  • Page 14 fingerprints. which current can bypass the gel.) Pre-wet nitrocellulose or nylon membranes with distilled water. Pre-wet PVDF or other hydrophobic membranes with methanol. Then soak all membrane types in transfer buffer for 2– 5 minutes. • TE70X-IMA0...
  • Page 15 Complete the stack Important! Stack each layer with care, with edges parallel. As each a) Place the pre-wet membrane onto the stack of layer is added, remove all air pock- blotting paper. ets by rolling a clean pipet from b) Place the gel on the membrane. Note: Proteins bind the center to the edges.
  • Page 16 Place the Cover on top of the transfer stack. Do not remove the cover until after the transfer is complete in order to prevent stack components from moving. Blotting paper, (2–3 sheets) Membrane Blotting paper, (2– 3 sheets) Mask (optional) • TE70X-IMA0...
  • Page 17 Blotting paper, (2–3 sheets) Membrane Blotting paper, (1 sheet) Cellophane Membrane Blotting paper, (2– 3 sheets) Mask (optional) •...
  • Page 18 Electrotransfer TE70X and TE77X Note: Transfers exceeding one The power supply should be switched off and both the hour may require additional current and voltage controls set at zero sheets of buffer saturated blot paper in the transfer stack. Then plug the color-coded leads from the base of the transfer unit into the power supply jacks, matching red to red and black to black.
  • Page 19 TE70XP and TE77XP Turn the instrument on using the POWER button on the front keypad. The instrument works by setting the current and time, and then starting the transfer. During the transfer, the voltage can be displayed. The trans- fer can not be set to run at constant voltage. Note: While setting the time, there is an extra setting labelled The DISPLAY MODE BUTTON toggles...
  • Page 20 If desired, the cover can be removed, and buffer can be added to the transfer stack. Replace cover, and press any key (except Dry error message. Power ON/OFF) to continue. For future transfers, add more layers of buffer soaked blot paper. • TE70X-IMA0...
  • Page 21 After the transfer is complete TE70X and TE77X Turn off the power supply and disconnect the leads from the power supply. TE70XP and TE77XP After a transfer is complete, the unit will beep for 5 seconds. The LED display will blink and cycle between the values for the current, time and voltage at the time the transfer was stopped.

  • Page 22: Care And Maintenance

    Rinse the cathode in the cover and the anode in the base with distilled water. Let the unit air dry completely. If using radioactive reagents, decontaminate the unit with a cleaning agent such as Contrad 70. Never use abrasive ™ cleansers. • TE70X-IMA0...

  • Page 23: Troubleshooting

    4. Troubleshooting problem solution Incomplete transfer Blank or faint areas on Remove trapped air pockets between the gel and the membrane membrane during stack assembly. Use buffer with a lower ionic strength. Molecules do not migrate Check all electrical connections. Confirm that current out of gel is flowing through the transfer stack.
  • Page 24 Open circuit or no output current Blown fuse in protection circuit. for TE70X or TE77X Replace fuse, (see Accessories in Ordering informa- tion on page 22). Smeared or diffuse band patterns If equilibrating before the transfer, shorten or elimi- nate the equilibration time and/or equilibrate under coldroom conditions.
  • Page 25 problem solution Inefficient binding to membrane Chemical parameters Prepare transfer buffer without SDS. (SDS can improve transfer efficiency but can interfere with protein binding to a PVDF membrane.) Add 10 – 20% methanol to the transfer buffer to enhance binding to nitrocellulose or PVDF. Membrane parameters Use a membrane with a smaller pore size (0.20 µm) if proteins pass through the membrane.

  • Page 26: Electrotransfer Notes

    Bring to 1 liter with distilled water. Do not adjust the pH, which should be between 8.2 – 8.4. Optional: Chill before use. *Optional: Adding SDS can improve transfer efficiency. † Depending on the membrane type selected (see table above), adding methanol can improve transfer results. • TE70X-IMA0...
  • Page 27 CAPS buffer, 1X (10 mM CAPS, pH 11.0, 1 liter) CAPS (FW 221.3) 10 mM 2.2 g [3-(cyclohexylamino)-1-propanesulfonic acid] Dissolve in 600 ml distilled water, adjust to pH 11.0 with conc. NaOH. Adjust volume to 1.0 liter. • For a 3-buffer system, refer to Kyhse- Anderson, J.

  • Page 28: Bibliography

    Smejkal, G., and Gallagher, S., Determination of Semidry Protein Transfer Efficiency with Transverse Gradient Gel Electrophoresis. Biotechniques. 16, 196–202 (1994). Tovey, E. and Baldo, B., Comparison of semi-dry and conventional tank-buffer electrotransfer of proteins from polyacrylamide gels to nitrocellulose membranes. Electrophoresis 8, 384–387 (1987). • TE70X-IMA0...

  • Page 29: Customer Service Information

    No items can be arrange to repair it, please call or fax your local accepted for servicing or return unless this form is properly Hoefer, Inc. representative. completed. Check the Hoefer, Inc. website for the distributor in you area. www.hoeferinc.com •...

  • Page 30: Ordering Information

    8. Ordering information product qty. order no. TE70X Semi-dry Transfer Unit, 14 × 16 cm TE70X Includes 25 sheets of blotting paper, 50 sheets of cellophane, 2 solid masks, masks for 7 × 8 cm and for 14 × 16 cm gels TE77X Semi-dry Transfer Unit, 21 ×...
  • Page 31 Related products product order no. SE600 Chroma SE600X-15-1.5 MiniVE Vertical Electrophoresis system SE300-10A-1.0 PS 2A200 Power Supply PS2A200 Transfer Membranes Pure Nitrocellulose, sheets and roll 0.45 µm pore size 8 × 9.5 cm, 10 sheets GM-NC45-89 16 × 16 cm, 10 sheets GM-NC45-1616 20 ×...
  • Page 32 Notes: • TE70X-IMA0...
  • Page 33 Notes: •...
  • Page 34 Notes: • TE70X-IMA0...
  • Page 36 Tween is a trademark of ICI Americas Inc. Coomassie is a trademark of ICI plc. Hoefer, Inc. 953 Indiana Street San Francisco, CA 94107 USA www.hoeferinc.com © 2005 Hoefer, Inc. — All rights reserved. Printed in the USA • TE70X-IMA0...

This manual is also suitable for:

Te77xTe70xpTe77xp

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