Fluidigm Access Array User Manual page 40

Chapter 6: Post-PCR Amplicon Purification and Quantitation
PCR Product Library Quantitation Procedure
3 Continue to quantify the pooled, purified library by calculating the PCR Product Library
Concentration outlined later in this chapter.
4 Once each IFC Library has been purified and quantified, normalize the concentration of
each IFC Library and pool libraries volumetrically by adding an equal volume of each
library to a new microcentrifuge tube.
The pooled Access Array IFC library is now ready for sequencing.
40 Access Array System for the Ion Torrent PGM Sequencing System: User Guide