Required Equipment; Example Sample And Primer Layout In 96-Well Plates - Fluidigm Access Array User Manual

Chapter 1: Access Array System Overview

Example Sample and Primer Layout in 96-Well Plates

Consumables from Other Suppliers
Product Name
Microcentrifuge tubes, 1.5 mL
96- and 384-well PCR plates
Adhesive seals for PCR plates
P2-P1000 pipette tips

Required Equipment

Equipment Supplied by Fluidigm
Product Name
IFC Controller AX (2 quantity,
FC1 cycler
NOTE The stand-alone thermal cycler has been discontinued. It has
been replaced by the FC1 cycler.
Equipment from Other Suppliers
Product Name
DynaMag™-2 Magnet
Agilent 2100 Bioanalyzer®
Microcentrifuge
Vortex mixer
Plate centrifuge
96- and 384-well PCR thermal cycler
Single-channel P2-P1000 pipettes
8-channel P20 pipette
Example Sample and Primer Layout in 96-Well Plates
For ease of pipetting, fill the 96-well plates by columns, not rows.
14
pre-PCR and post-PCR)
Access Array System for the Ion Torrent PGM Sequencing System: User Guide
Manufacturer Part Number
MLS —
MLS —
MLS —
Rainin —
Manufacturer Part Number
Fluidigm IFC-AX
Fluidigm CYC-FC1
Manufacturer Part Number
Thermo Fisher 123-21D
Scientific
Agilent G2939BA
MLS —
MLS —
MLS —
MLS —
Rainin —
Rainin —