Fluidigm Access Array Manuals

Fluidigm Access Array Manuals

Manuals and User Guides for Fluidigm Access Array. We have 1 Fluidigm Access Array manual available for free PDF download: User Manual

Fluidigm Access Array User Manual

Fluidigm Access Array User Manual (51 pages)

for the Ion Torrent PGM Sequencing System

Brand: Fluidigm | Category: Laboratory Equipment | Size: 1.25 MB

Table of Contents

Table of Contents
3
About this Guide
5
Safety Alert Conventions
5
Safety Data Sheets
6
Chapter 1: Access Array System Overview Access Array System Components

10
- Access Array System Components
  10
- Access Array Ifcs
  11
- Specifications
  11
- Access Array System Workflow
  12
- Materials
  12
- Required Reagents
  12
- Required Consumables
  13
- Required Equipment
  14
- Example Sample and Primer Layout in 96-Well Plates
  14
- Before You Begin
  15
Chapter 2: Designing Targeted Sequencing Primers

16
Chapter 3: Target-Specific Primer Validation for 4-Primer Amplicon Tagging on the LP 48.48

17
Chapter 4: Sample Quantitation and Normalization

22
- Reference Documents
  22
- Materials
  22
- Required Reagents
  22
- Required Equipment
  22
- Sample Quantitation
  23
- Sample Normalization
  23
Chapter 5: Bidirectional Amplicon Tagging on the LP 48.48

24
- Reference Documents
  25
- Materials
  25
- Required Reagents
  25
- Prime the IFC
  26
- Prepare the 20X Primer Solutions
  28
- Prepare Sample Pre-MIX and Samples
  29
- Prepare the Sample Pre-MIX Solution
  29
- Prepare the Sample MIX Solutions
  29
- Load the IFC
  30
- Thermal-Cycle the IFC
  31
- Harvest the IFC
  31
- Attach Sequence Tags and Sample Barcodes
  33
- Prepare the Sample Pre-MIX Solution
  33
- Prepare a 100-Fold Dilution of the Harvested PCR Products
  33
- Prepare the Sample MIX Solutions
  33
- Thermal-Cycle the 96-Well PCR Plate
  34
- Check PCR Products on the Agilent 2100 Bioanalyzer
  35
- Next Step
  35
Chapter 6: Post-PCR Amplicon Purification and Quantitation

36
Appendix A: Electropherogram Examples

41
Appendix B: Access Array Barcode Library for the Ion Torrent PGM Sequencer

43
Appendix C: Related Documents

47
Appendix D: Safety

48
- General Safety
  48
- Instrument Safety
  48
- Symbols on the Instrument
  49
- Electrical Safety
  50
- Chemical Safety
  50

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