Fluidigm CyTOF XT Manuals

Fluidigm CyTOF XT Manuals

Manuals and User Guides for Fluidigm CyTOF XT. We have 1 Fluidigm CyTOF XT manual available for free PDF download: User Manual

Fluidigm CyTOF XT User Manual

Fluidigm CyTOF XT User Manual (148 pages)

Brand: Fluidigm | Category: Laboratory Equipment | Size: 7.59 MB

Table of Contents

Table of Contents
3
About this User Guide
5
Safety Alert Conventions
5
Safety Alerts for Chemicals
5
Safety Alerts for Instruments
6
Safety Data Sheets
6
Chapter 1: Product Overview

7
Chapter 2: Mass Cytometry and Cytof XT

16
- Cytof XT Instrument Overview
  16
- Autosampler Module
  17
- Cytof XT System Components Overview
  17
- Autosampler Module Functions
  25
- Nebulizer Assembly
  28
- Heater Assembly
  30
- Ionization
  31
- Vacuum Interface
  34
- Event Detection and Acquisition Data File Format
  36
- Data Acquisition and Analysis
  37
- Chapter 3: Annotated Figures of Cytof XT Components
  
  38
- Main Components of the Cytof XT Instrument
  38
- Cytof XT Status Panel
  39
- Autosampler Module
  40
- Nebulizer Assembly
  44
- Heater Assembly
  45
- Torch Assembly
  46
- Interior View of Cytof XT
  47
- Other Components of Cytof XT
  48
Chapter 4: Maintenance

52
- Cytof XT Routine Cleaning
  52
- Clean the Nebulizer
  53
- Remove the Nebulizer
  54
- Materials Required for Cleaning
  56
- Daily Cleaning Method: 10% Contrad/Decon
  56
- Cleaning Method for Manual Clog Removal: Hot DIW
  58
- Clean the Nebulizer Rest
  60
- Clean the Injector and Spray Chamber
  60
- Remove the Heater Assembly
  61
- Remove the Injector Cap and Injector
  62
- Clean the Injector
  63
- Remove the Spray Chamber and Injector Holder
  64
- Clean the Spray Chamber and Injector Holder
  66
- Clean the Vacuum Interface Cones
  66
- Remove Cones
  67
- Clean Cones
  70
- Reinstall Cones
  72
- Reassemble the Injector and Spray Chamber
  74
- Replace the Nebulizer Port O-Ring
  79
- Check the Nebulizer Holder O-Ring
  81
- Reassemble the Nebulizer Assembly
  83
- Clean the Autosampler Fluidic Lines and Nebulizer with 10% Bleach
  85
- Cleaning Summary
  88
- Maintenance Checklist
  89
- Heater Assembly and Spray Chamber
  89
- Injector
  89
- Cones
  89
- Periodic Maintenance
  90
- Autosampler Module Maintenance
  91
- Replace the Sample Loop
  91
- Replace the Probe Line
  92
- Recalibrate the Probe Line
  93
- Replace a Bottle Line
  97
- Clean the Carousel
  98
- Clean the Bottle Tray
  101
- Replace Syringes
  102
- Nebulizer Assembly Maintenance
  108
- Replace the Nebulizer Line
  108
- Replace the Nebulizer Gas Line
  111
- Clean the Nebulizer Mount
  111
- Remove and Clean the Torch Assembly
  116
- Remove and Disassemble Torch Assembly
  116
- Clean the Inner and Outer Torch
  121
- Reassemble the Torch Assembly
  122
- Fill the Chiller with Coolant
  124
- Check the Cooling System Air Filter
  127
- Check the Autosampler Air Filter
  129
Appendix A: Troubleshooting

132
Appendix B: Recommendations for Extended Shutdown

137
- Expected Power Outages
  137
- Cytof XT Shutdown Procedure
  137
- Cytof XT Startup Procedure
  138
Appendix C: Preventive Maintenance, Decontamination, and Disposal

139
- Preventive Maintenance Recommendations
  139
- Clean and Decontaminate the Cytof XT System
  139
- Clean the System
  139
- Dispose of Products
  140
Appendix D: Related Documents

141
Appendix E: Revision History

142
Appendix F: Safety

143
- General Safety
  143
- Instrument Safety
  143
- Symbols Used in Documentations and on the Instrument
  144
- Electrical Safety
  145
- Chemical Safety
  146
- Laboratory Ventilation Safety
  146
- Pressurized Gas Safety
  147
- Safe Handling of Gas Cylinders
  147
- Sample Handling and Preparation Safety
  147
- Radio Frequency Radiation Safety
  147

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