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Fluidigm Juno PCR Automation System Manuals
Manuals and User Guides for Fluidigm Juno PCR Automation System. We have
2
Fluidigm Juno PCR Automation System manuals available for free PDF download: User Manual, Getting Started Manual
Fluidigm Juno User Manual (62 pages)
Brand:
Fluidigm
| Category:
Laboratory Equipment
| Size: 2.96 MB
Table of Contents
Table of Contents
3
About this Guide
5
Purpose
5
Safety Alerts for Chemicals
5
Safety Alerts for Instruments
5
Safety Data Sheets
6
Chapter 1: Introducing the Juno System
7
The Juno System
7
Consumables Ordering
7
Components of the Juno System
8
Interface Plates and Ifcs
10
System Software
10
Scripts
11
Basic Scripts
11
Advanced Scripts
14
Components Included in Shipping Box
15
Optional Components
15
System Functions
16
Air Options
16
Regulatory Compliance
16
General Regulations and Requirements
16
Harmonized Standards
16
Conformity Symbols on the Instrument
17
Chapter 2: Getting Started with Juno
18
Start the Juno System
18
How to Use the Juno Touchscreen
20
Start a Run
20
Load an IFC
20
Select a Script
23
Schedule the Harvest with the LP 192.24 IFC or LP 48.48 IFC
24
Run a Script
24
Switch an Interface Plate
26
Install an Interface Plate
29
View System Information
32
Export a Log
33
Shut down the System and then the Instrument
34
Chapter 3: Using Advanced Features of Juno
36
Log in
36
Log out
36
Manage Scripts
37
Enable Advanced Scripts
37
Export Scripts to a USB
37
Install Scripts from a USB
38
Modify Scripts
39
To View the Scripts to Modify
39
To Change General Properties of a Script
40
To Change Thermal Properties of a Script
40
To Remove a Script
42
Manage Users
42
Change the Date and Time
44
Update the System
45
Enable In-House Air
46
Appendix A: Troubleshooting
47
Powering ON/OFF
47
Loading
48
Running
49
Cleaning
49
Appendix B: Preventive Maintenance, Decontamination, and Disposal
50
Preventive Maintenance
50
Preventive Maintenance Recommendations
50
Clean the Thermal Cycler Chuck
50
Clean an Interface Plate
51
Clean the Juno System
53
Clean the Touchscreen
55
Replace the Fuses
55
Decontamination and Disposal
56
Biological Agents
56
Hazardous Chemicals
56
Radioactive Materials
56
Disposal of Products
57
Appendix C: Related Documentation
58
Appendix D: Safety
59
General Safety
59
Instrument Safety
59
Symbols on the Instrument
60
Electrical Safety
60
Chemical Safety
61
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Fluidigm Juno Getting Started Manual (32 pages)
Brand:
Fluidigm
| Category:
Laboratory Equipment
| Size: 1.54 MB
Table of Contents
Table of Contents
3
About this Guide
4
Purpose
4
How to Use this Guide
5
Safety Alert Conventions
5
Safety Alerts for Chemicals
5
Safety Alerts for Instruments
5
Safety Data Sheets
6
Best Practices
7
Getting Started
7
Related Documentation
7
Workflow
7
Chapter 1: Product Information
8
Required Kit Contents
8
Taqman Assay Kit
8
SNP Type Assay Kit
9
Required Reagents
10
Taqman Assays
10
SNP Type Assays
10
Suggested Reagents
10
Required Consumables
11
Required Equipment
11
Suggested Equipment
11
Required Software
12
IFC Type and Related Scripts
12
Chapter 2: Genotyping with the Juno 96.96 Genotyping IFC Using Taqman Assays
13
Prepare Assay and Sample Mixes
13
Prepare the Primer Pool for Preamplification
13
Prepare 2X Taqman Assays for Genotyping
14
Prepare the Assay MIX
14
Obtain the Minimum Required Genomic DNA
14
Prepare the Sample MIX
15
Load and Run the IFC on Juno
16
Perform Genotyping Analysis on the Samples
19
Chapter 3: Genotyping with the Juno
20
Genotyping IFC Using SNP Type Assays
20
Prepare Assay and Sample Mixes
20
Prepare the 200 Nm Primer Pool for Preamplification
20
Prepare 2X SNP Type Assays
21
Prepare the Assay MIX
22
Obtain the Minimum Required Genomic DNA
22
Prepare the Sample MIX
23
Load and Run the IFC on Juno
24
Perform Genotyping Analysis on the Samples
27
Appendix A: Suggested Kits
28
Taqman Assay Kit
28
SNP Type Assay Kit
28
Suggested Reagents to Use with Taqman Assay and SNP Type Assay Kits
29
Appendix B: Safety
30
General Safety
30
Instrument Safety
30
Electrical Safety
31
Chemical Safety
31
Disposal of Products
31
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