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The continuous development of our meter software is part of the process of consequently improving our products. The current meter software for the inoLab pH/ION 735 can be found on the Internet. It can easily be transmitted to your meter with the aid of the supplied AK 340/B cable and a PC.
Overview Overview General features The inoLab pH/ION 735 compact precision pH meter enables you to carry out pH measurement, ORP measurements and concentration measurements with ion sensitive electrodes quickly and reliably. The inoLab pH/ION 735 provides the maximum degree of operating comfort, reliability and measuring certainty for all applications.
Cancel inputs Store a measured value Switch between pH measurement and ORP measurement, in- crease values, scroll Confirm entries, start AutoRead Switch between pH measurement and ORP measurement, decrease values, scroll * only on the inoLab pH/ION 735 with integrated printer...
735 Overview Display The graphical display can indicate up to three measuring windows at the same time. The illumination enables to read the display even in the darkness. You can modify the display to meet your requirements in multiple ways.
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735 Warning Only connect sensors to the meter that cannot return any voltages or currents that are not allowed (> SELV and > current circuit with current limiting). Almost all sensors - in particular WTW sensors - fulfill these conditions.
735 Safety Safety This operating manual contains basic instructions that you must follow during the commissioning, operation and maintenance of the meter. Consequently, all responsible personnel must read this operating man- ual before working with the measuring system. The operating manual must always be available within the vicinity of the measuring system.
Safety inoLab pH/ION 735 General safety instructions This instrument is built and inspected according to the relevant guide- lines and norms for electronic meters (see chapter 7 T ECHNICAL DATA It left the factory in a safe and secure technical condition.
735 Commissioning Commissioning Scope of delivery ! inoLab pH/ION 735 laboratory meter ! Operating manual and short instructions in the printer cover ! 4 NiMH rechargeable batteries 1.2 V Mignon type AA ! Power pack Power supply Line-powered operation...
When you set the language, date and time according to the mentioned sections of this operating manual you will quickly be familiar with the simple operation of the inoLab pH/ION 735. * only on the inoLab pH/ION 735 with integrated printer...
735 Operation Operation Switching on the meter Switching on Press the e key. The measured value display appears. 6.949 25.0 °C 20.09.04 10:37 Switching off Press the e key. Automatic switchoff The instrument has an automatic switchoff function in order to save the batteries (see section 4.3.2).
Operation inoLab pH/ION 735 General operating principles This section contains basic information of the operation of the inoLab pH/ION 735. Operating elements, An overview of the operating elements and the display is given in display section 1.2 and section 1.3.
735 Operation 4.2.2 Navigation Measured value display In the measured value display, you can ! select a measuring window with 82 and open the relevant measuring menu by shortly pressing g. ! open the system menu with the sensor-independent settings by pressing g for a long time (approx.
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Operation inoLab pH/ION 735 ! Functions Functions are designated by the name of the function. They are im- mediately carried out by confirming with g. Example: Display the Calibration record function. Cal. type Calibration interval Unit for slope mV/pH Calibration record i 2.00 4.01 7.00 10.01...
735 Operation 4.2.3 Navigation example 1: Setting the language Press the e key. The measured value display appears. The instrument is in the measuring mode. 7.00 25.0 °C 20.09.04 10:37 Open the system menu by pressing g for a long time (approx.
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Operation inoLab pH/ION 735 Open the General submenu with g. System Language Deutsch Beep Illumination Contrast 48 % Temperature unit °C Switchoff time 30 min Open the setting mode for the Language with g. System Language Deutsch Beep Illumination Contrast...
735 Operation 4.2.4 Navigation example 2: Setting the date and time The meter has a clock with a date function. The date and time are indi- cated in the status line of the measured value display. The indication can be switched off.
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Operation inoLab pH/ION 735 Change and confirm the setting with 82 and g. The time is set. If necessary, set the Date and Date format. The setting is made similarly to that of the time. If necessary, select and set the Date with 82 and g.
735 Operation System settings (system menu) The system menu comprises the following settings: ! Data storage (see section 4.3.1) ! System (see section 4.3.2). 4.3.1 Data storage This menu contains all functions to display, edit and erase stored mea- sured values and calibration records.
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Operation inoLab pH/ION 735 Menu item Setting Description System / General / Il- Switching the display illu- lumination mination on/off System / General / 0 ... 100 % Changing the display con- Contrast trast System / General / °C Temperature unit, Temperature unit °F...
735 Operation pH value / ORP voltage 4.4.1 General information You can measure the following variables: ! pH value [ ] ! ORP [mV] Warning When connecting an earthed PC/printer, measurements cannot be performed in earthed media as incorrect values would result. The RS232 interface is not galvanically isolated.
Operation inoLab pH/ION 735 Preparatory activities Perform the following preparatory activities when you want to measure: Connect a pH or ORP electrode to the meter. The pH/ISE measuring window is displayed. If necessary, select the pH or mV display with m.
735 Operation 4.4.3 Measuring the ORP voltage Note ORP electrodes are not calibrated. However, you can check ORP elec- trodes using a test solution. Perform the preparatory activities according to section 4.4.1. Submerse the ORP electrode in the sample.
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Operation inoLab pH/ION 735 Settings The settings are made in the measuring menu of the pH/ORP measure- ment. To open the settings, activate the relevant measuring window in the measured value display and press the g key shortly. After com- pleting the settings, switch to the measured value display with m.
735 Operation 4.4.5 pH calibration Why calibrate? pH electrodes age. This changes the asymmetry (zero point) and slope of the pH electrode. As a result, an inexact measured value is dis- played. Calibration determines the current values of the asymmetry and slope of the electrode and stores them in the meter.
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Operation inoLab pH/ION 735 Calibration points Calibration can be performed using one, two or three buffer solutions in any order (single-point-, two-point or three-point calibration). The meter determines the following values and calculates the calibration line as follows: Determined val-...
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735 Operation Sample record: 31.08.04 16:13 inoLab pH/ION 735 Ser. no. 12345678 CALIBRATION pH Calibration date 01.08.04 00:14:03 Cal. interval AutoCal TEC Buffer 1 7.00 Voltage 1 -0.2 mV 26.3 øC Slope -59.2 mV/pH Asymmetry -0.5 mV Sensor Calibration evaluation After calibrating, the meter automatically evaluates the calibration.
(see section 4.4.5). Make sure that the Cal. type is correctly set in the sensor menu (see section 4.4.4). For this procedure, use any one, two or three WTW technical buffer so- lutions in ascending or descending order.
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735 Operation Select Continue with 82 and press g. The buffer is mea- sured. The measured value is checked for stability (AutoRead). pH <CAL> Displayed: i Buffer value = 7.000 – Recognized nominal buffer i U = 3.0 mV value (referring to 25 °C)
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Operation inoLab pH/ION 735 Continuing for two-point Thoroughly rinse the electrode with distilled water. calibration (Cal. type TEC) Immerse the electrode in buffer solution 2. If the Set temperature menu item appears, measure and enter the temperature of the buffer manually (measurement without temperature sensor).
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735 Operation Continuing for three- Thoroughly rinse the electrode with distilled water. point calibration to six- point calibration Immerse the electrode in buffer solution 3. (Cal. type TEC) If necessary, measure the temperature of buffer 3 manually, then enter and confirm it with 82 and g in the Set temper- ature setting.
Operation inoLab pH/ION 735 4.4.7 Carrying out a ConCal calibration Single-point calibration Use any buffer solution for this rapid method. The calibration will be exacter if the pH value of the buffer solution is closer to that of the test sample.
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735 Operation pH <CAL> i Buffer value = 6.80 i U = 12.0 mV i Temperature = 24.8 °C Terminate AutoRead Wait for the end of the AutoRead measurement or Terminate AutoRead and accept the calibration value with g.
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Operation inoLab pH/ION 735 Continuing for two-point Thoroughly rinse the electrode with distilled water. calibration (Cal. type Immerse the electrode in buffer solution 2. ConCal) Set the nominal buffer value with g and 82. Then confirm the value with g.
735 Operation Ion concentration 4.5.1 General information Note Incorrect calibration of ion sensitive electrodes will result in incorrect measured values. Calibrate regularly before measuring. Warning When connecting an earthed PC/printer, measurements cannot be performed in earthed media as incorrect values would result. The RS232 interface is not galvanically isolated.
Operation inoLab pH/ION 735 4.5.2 Measuring the ion concentration Perform the preparatory activities according to section 4.5.1. Immerse the electrode in the test sample. 0.157 mg/l °C 20.09.04 10:37 AutoRead The AutoRead function (drift control) continually checks the stability of (drift control) the measurement signal.
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735 Operation Menu item Possible set- Description ting Calibration record Displays the calibration record of the last calibra- tion. Man. temperature -20 ... +130 °C Entry of the manually de- termined temperature. For measurements without temperature sensor only.
Operation inoLab pH/ION 735 4.5.4 Calibrating for ISE measurements Why calibrate? Ion-selective electrodes age and are temperature-dependent. This changes the slope. As a result, an inexact measured value is displayed. Calibration determines the calibration line of the electrode and stores this value in the meter.
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735 Operation After accepting the new calibration data the calibration record is dis- played. Note Based on the calibration data, the calibration line is determined in sec- tions. A straight line of the calibration is determined according to the Nernst equation between the highest and second highest calibration standard.
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Operation inoLab pH/ION 735 Preparatory activities Perform the following preparatory activities when you want to calibrate: Connect the ISE combination electrode to the meter. The pH/mV/ISE measuring window is displayed. Keep the standard solutions ready. If necessary, measure the temperature of the standard solu- tions with a thermometer.
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735 Operation ISE <CAL> i Standard = 0.010 mg/l i U = 0.5 mV Terminate AutoRead Wait for the end of the AutoRead measurement or accept the calibration value with g. The calibration display for the next standard solution appears.
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Operation inoLab pH/ION 735 Wait for the end of the AutoRead measurement or accept the calibration value with g. The calibration display for the next standard solution appears. ISE <CAL> i #2 0.020 mg/l 24 °C i Immerse sensor in std. 3 Set temperature: 24 °C...
735 Operation 4.5.5 Selecting the measuring method The following methods are supported: ! Standard addition (Std. add.) ! Standard subtraction (Std. sub.) ! Sample addition (Sample add.) ! Sample subtraction (Sample sub.) ! Blank value addition (Blank add.) Perform the preparatory activities according to section 4.5.1.
Operation inoLab pH/ION 735 4.5.6 Standard addition (Std. add.) In the "Standard addition" procedure, a known amount of standard so- lution is added to the sample. The ion concentration in the sample is calculated from the change in potential. Select the measuring method (see section 4.5.5).
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735 Operation Standard addition i Add standard i U = 19.0 mV Continue Add the standard solution to the sample. Start the measurement with g. When the measurement is finished an entry window appears. Standard addition Std. volume 1.0 ml...
Operation inoLab pH/ION 735 4.5.7 Standard subtraction (Std. sub.) In the "Standard Subtraction" procedure, a known amount of standard solution is added to the sample (as complexing agent or precipitating agent) and, thus, the ion concentration lowered. The ion concentration in the sample is calculated from the change in potential.
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735 Operation Standard subtraction i Add standard i U = 19.0 mV Continue Add the standard solution to the sample. Start the measurement with g. When the measurement is finished an entry window appears. The info line indicates the current setting for the ion sensitive electrode [e.g.
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Operation inoLab pH/ION 735 If necessary, set the ion type for the ion in the sample (Ion type (ISE)) with 82 and g. The relevant data Valency and Molar mass is displayed auto- matically. With selection of the ion type that can be defined (??): Using 82 and g, set the valence (Valency) and molar weight (Molar mass) for the ion in the standard solution.
735 Operation 4.5.8 Sample addition (Sample add.) In the "Sample addition" procedure, a known amount of sample is add- ed to the standard solution. The ion concentration in the sample is calculated from the change in potential. Select the measuring method (see section 4.5.5).
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Operation inoLab pH/ION 735 Sample addition i Add sample i U = 22.0 mV Continue Add the sample to the standard solution. Start the measurement with g. When the measurement is finished an entry window appears. Sample addition Sample volume 1.0 ml...
735 Operation 4.5.9 Sample subtraction (Sample sub.) In the "Sample Subtraction" procedure, a known amount of sample is added to the standard solution. The ion concentration in the sample is calculated from the change in potential. The sample subtraction is one method for the indirect determination of ions.
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Operation inoLab pH/ION 735 Select Continue with 82 and start the measurement with g. When the measurement is finished an info window appears. Sample subtraction i Add sample i U = 22.0 mV Continue Add the sample to the standard solution.
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735 Operation If necessary, set the ion type for the ion in the sample ( Ion type (ISE)) with 82 and g. The relevant data Valency and Molar mass is displayed auto- matically. With selection of the ion type that can be defined (??): Using 82 and g, set the valence (Valency) and molar weight (Molar mass) for the ion in the standard solution.
Operation inoLab pH/ION 735 4.5.10 Standard addition with blank value correction (Blank add.) In the "Standard addition with blank value correction" procedure, a known amount of standard solution is added to the sample in two steps. With the first addition, the ion concentration is raised to the linear range of the electrode characteristic curve.
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735 Operation Using 82 and g, enter the values for the volume of the sample (Sample volume), the volume of the ISA/TISAB solu- tion (ISA/TISAB vol.), the volume of the blank value solution (BV volume) and the concentration of the blank value solution (BV concentration).
Operation inoLab pH/ION 735 Terminate the measuring method with #. A security prompt appears. Select Yes with 82. Confirm Yes with g. Measurement with the selected method is completed. Storage You can transmit measured values (data records) to the data storage in two ways: ! Manual storage (see section 4.6.1)
735 Operation 4.6.1 Manual storage Proceed as follows to transmit to the data storage and simultaneously output to the interface a measurement dataset: Press the <STO> key shortly. The menu for manual storage appears. <STO> 1 of 646 - 30.10.2003 11:24:16...
Operation inoLab pH/ION 735 4.6.2 Automatic storage at intervals The storage interval (Interval) determines the chronological interval be- tween automatic storage processes. Each storage process transmits the current dataset to the interface at the same time. Configuring the auto- Press the <STO> key for a long time.
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735 Operation Storage administration The two lower display lines indicate the use of the storage calculated in advance for the selected settings: <STO_> Store with ID no. Adjusted storage duration for the Interval 30 s entire dataset series...
Operation inoLab pH/ION 735 Terminating the auto- Proceed as follows to switch off the automatic storage function before matic storage function the adjusted storage duration has expired: prematurely Press the <STO> key for a long time. The following window appears.
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735 Operation Settings Menu item Setting/func- Description tion Data storage / Displays in pages all mea- Manual data storage / suring datasets that corre- Display spond to the filter settings. Further options: ! Scroll through the datasets with 82.
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Operation inoLab pH/ION 735 Data filter Menu item Setting/func- Description tion Filter Filter criteria: No filter Data filter switched off Date & ID Selection according to pe- number riod and ID number ID number Selection according to ID number Date...
Operation inoLab pH/ION 735 4.6.5 Displaying and outputting calibration records You can display the calibration data ! of one selected sensor, or ! of all sensors connected and then output them to the interface. Displaying the calibra- The calibration record of the last calibration can be found under the Cal-...
Operation inoLab pH/ION 735 Transmitting data (RS 232 interface) 4.7.1 Options for data transmission Via the RS 232 interface, you can transmit data to a PC or an external printer. The following table shows which data are transmitted to the in-...
735 Operation 4.7.2 Connecting a PC/external printer Use the AK340/B (PC) or AK325/S (ext. printer) cable to connect the interface to the devices. Warning The RS232 interface is not galvanically isolated. When connecting an earthed PC/printer, measurements cannot be performed in earthed media as incorrect values would result.
Operation inoLab pH/ION 735 Reset You can reset (initialize) all sensor settings and sensor-independent settings separately from each other. 4.8.1 Resetting the sensor settings Note The calibration data are reset to the default settings together with the measuring parameters. Recalibrate after performing a reset.
735 Operation 4.8.2 Resetting the system settings The following system settings can be reset to the delivery status: Setting Default settings Language English Temperature unit °C Beep Baud rate 4800 Baud Output format ASCII Contrast 40 % Illumination...
735 Maintenance, cleaning, disposal Maintenance, cleaning, disposal Maintenance The only maintenance activities are: ! exchanging the rechargeable batteries and ! exchanging the roll of printer paper. Note For maintenance of the electrodes refer to the relevant operating man-...
Maintenance, cleaning, disposal inoLab pH/ION 735 5.1.1 Exchanging the rechargeable batteries Warning Always use the specified NiMH rechargeable batteries (see chap- ter 7 T ). Using normal batteries can damage the ECHNICAL DATA meter. Open the battery compartment (1) on the underside of the meter.
(3) from below. Press the Print key f (paper feed). Close the printer lid (1). Note Use original WTW rolls of paper only. You will find information on this in the WTW catalog L or via the In- ABORATORY AND...
Maintenance, cleaning, disposal inoLab pH/ION 735 Disposal Packing This meter is sent out in a protective transport packing. We recommend: Keep the packing material. The original packing pro- tects the meter from transport damages. Rechargeable batteries Remove the rechargeable battery from the instrument and dispose of it at a suitable facility according to local legal requirements.
735 What to do if... What to do if... pH and ORP measurement Error message, Cause Remedy OFL or UFL Electrode: (measuring range exceeded or undercut) – Air bubble in front of the – Remove air bubble diaphragm –...
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What to do if... inoLab pH/ION 735 No stable measured Cause Remedy value pH electrode: – Diaphragm contaminated – Clean diaphragm – Membrane contaminated – Clean membrane Test sample – pH value not stable – Measure with air excluded if necessary –...
735 What to do if... ISE measurement Error message OFL Cause Remedy – Measuring range exceeded – Dilute test sample Obviously incorrect Cause Remedy measured values – Electrode not connected – Connect electrode – Cable broken – Replace electrode...
Printer operating - Cause Remedy paper not being printed * – Paper inserted with wrong side – Turn the roll of paper around upwards and insert it with the other side upwards only on the inoLab pH/ION 735 with integrated printer...
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735 What to do if... Display, LoBat Cause Remedy – Batteries almost empty – Charge the batteries (see section 3.2) Instrument does not Cause Remedy react to keystroke – Operating condition undefined – Processor reset: or EMC load unallowed Press the e and # key simultaneously.
735 Technical data Technical data General data Dimensions approx. 172 x 80 x 37 mm Weight approx. 0.3 kg (without power pack) Mechanical structure Type of protection IP 66 Electrical safety Protective class Test certificates cETLus, CE Ambient conditions Storage - 25 °C ...
735 Lists Lists This chapter provides additional information and orientation aids. Abbreviations The list of abbreviations explains the indicators and abbreviations that appear on the display and in the manual. Specialist terms The glossary briefly explains the meaning of the specialist terms. How- ever, terms that should already be familiar to the target group are not described here.
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Cal. type NIST/ Automatic pH calibration with buffer solutions pre- pared according to NIST or DIN 19 266 Cal. type TEC Automatic pH calibration with WTW technical buff- er solutions according to DIN 19267 °C Temperature unit, degrees Celsius Calibration...
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AutoRange Name of the automatic selection of the measuring range. AutoRead WTW name for a function to check the stability of the measured value. Calibration Comparing the value from a measuring system (e. g. the displayed value) to the correct value or a value that is regarded as correct. Of- ten, this expression is also used when the measuring system is adjust- ed at the same time (see adjusting).
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The slope of a linear calibration function. Slope (relative) Designation used by WTW in the DO measuring technique. It ex- presses the relationship of the slope value to the value of a theoretical reference sensor of the same type of construction.
735 Index Index ....27 ......74 Asymmetry of the pH electrode Exchanging the batteries ...........9 ..... 75 Authorized use Exchanging the roll of paper ......11, 13 Automatic switchoff AutoRead ............38 ............64 Filter ..........25 ........91 Firmware update .............24...
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Index inoLab pH/ION 735 Three-point calibration ........... 44 ..... 10 Obligations of the purchaser ............ 33 ........10 Operating safety ........68 Transmitting data ....68 Transmitting measured values Two-point calibration ......29 pH calibration evaluation ........... 43 ..........11 Power pack ..........
! a minimum of 10 MB free storage ! a free serial interface (COM port) on your PC ! the AK 340/B interface cable (part of the inoLab pH/ION 735 scope of delivery) ! the installation program to update the meter software.
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Appendix: Updating the meter software inoLab pH/ION 735...
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