BD 644787 User Manual
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BD 644787 User Manual

Hts customer care kits
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.
bdbiosciences.com
23-11165-00 Rev. A
1/2010
Becton, Dickinson and Company
BD Biosciences
San Jose, CA 95131
Tel 877.232.8995
Fax 800.325.9637
ResearchApplications@bd.com
HTS Customer Care
User's Guide
Asia Pacific
Tel 65.6.861.0633
Fax 65.6.860.1593
Europe
Tel 32.2.400.98.95
Fax 32.2.401.70.94
help.biosciences@europe.bd.com
Kits
Brazil
Tel 55.11.5185.9995
Fax 55.11.5185.9895
bdbiosciences.brasil@bd.com
Japan
Nippon Becton Dickinson Company, Ltd.
Toll Free 0120.8555.90
Tel 81.24.593.5405
Fax 81.24.593.5761
Canada
Toll Free 888.259.0187
Tel 905.288.6000
Fax 888.229.9918
canada@bd.com
Mexico
Toll Free 01.800.236.2543
Tel 52.55.5999.8296
Fax 52.55.5999.8288
biocienciasmexico@bd.com

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  • Page 1 For Research Use Only. Not for use in diagnostic or therapeutic procedures. bdbiosciences.com 23-11165-00 Rev. A 1/2010 Becton, Dickinson and Company Asia Pacific Brazil Canada BD Biosciences Tel 65.6.861.0633 Tel 55.11.5185.9995 Toll Free 888.259.0187 Fax 65.6.860.1593 Fax 55.11.5185.9895 Tel 905.288.6000 San Jose, CA 95131 bdbiosciences.brasil@bd.com...
  • Page 2 BD Biosciences. The information in this guide is subject to change without notice. BD Biosciences reserves the right to change its products and services at any time to incorporate the latest technological developments. Although this guide has been prepared with every precaution to ensure accuracy, BD Biosciences assumes no liability for any errors or omissions, nor for any damages resulting from the application or use of this information.
  • Page 3 History Revision Date Change Made 23-11165-00 Rev. A 1/2010 Initial release...

  • Page 5: Table Of Contents

    BD FACSCanto II System ........
  • Page 6 Chapter 5: Sample Coupler and Tubing Replacement BD FACSCanto and FACSCanto II Systems ......28 BD FACSCalibur, BD LSR II, or BD LSRFortessa Systems .

  • Page 7: Chapter 1: Overview Of Hts Customer Care

    HTS Customer Care Kits About the The HTS Customer Care kits can help keep the BD High Throughput Sampler Option (HTS) in peak operating condition. The HTS will experience problems if sheath fluid crystallization occurs in the connectors, tubing, and syringes.

  • Page 8: Kit Configurations

    LSR II (including special order BD LSR II) ™ Special order BD LSRFortessa When to Use the Kit The parts in the HTS Customer Care Kit can be used to resolve common problems. The following table is lists of symptoms, possible causes, and solutions that are described in this user’s guide.

  • Page 9: About The Hts

    About the HTS Illustrations of the HTS and a schematic of the fluidics system are provided for reference. For more detailed information, see your BD High Throughput Sampler User’s Guide. HTS Configurations The following figures show the front and rear views of the HTS for each cytometer.
  • Page 10 Figure 1-1 HTS for the BD FACSCanto II system Figure 1-2 HTS for the BD FACSCalibur and BD FACSCanto systems Note: The module body color for the BD FACSCanto is blue HTS Customer Care Kits User’s Guide...

  • Page 11: Location Of Commonly Clogged Components

    Figure 1-3 HTS for the BD LSR II, special order BD LSR II and BD LSRFortessa systems Note: The module body color might be unique to your system. NOTE The main HTS module shown is the same for all BD LSR products. The BD LSRFortessa analyzer has an extra protective cover that mounts to the original BD LSR II HTS cover.
  • Page 12 Figure 1-4 Schematic of the HTS fluidics system Pressure switch Flow cell Waste Primary pump pump Probe Wash station Tray Valve pump HTS Customer Care Kits User’s Guide...

  • Page 13: Safety And General Precautions

    Safety and General Precautions Biological Safety All instrument surfaces that come in contact with biological specimens can transmit potentially fatal disease. Use universal safety precautions when changing tubing or removing any part in contact with the fluidics system. Wear suitable protective clothing, eyewear, and gloves. Electrical Safety Ensure that the power to the HTS is turned off before handling electrical cables and their connections.
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  • Page 15: Chapter 2: Hts Removal And Reinstallation

    All instrument surfaces that come in contact with biological specimens can transmit potentially fatal disease. BD FACSCanto II System Removing the HTS Turn off the cytometer and HTS. Remove or open the BD High Throughput Sampler safety cover. Chapter 2: HTS Removal and Reinstallation...
  • Page 16 Detach the sample coupler from the cytometer SIT. Unscrew the top nut. Pull the sample coupler down and away from the SIT, leaving the nut attached to the sample coupler. Unscrew the captive screw located at the bottom of the HTS support bracket.

  • Page 17: Reinstalling The Hts

    Place the HTS unit on a stable work bench. Sheath tubing connection Waste tubing Interlock cable connection connection Interface cable connection Sampler coupler Reinstalling the HTS Place HTS on support bracket. Connect the interlock cable to the HTS. Connect the interface cable to the HTS. Connect the waste tubing to the waste connector on the HTS.

  • Page 18: Bd Facscanto, Bd Lsr Ii, Bd Facscalibur, And Bd Lsrfortessa Systems

    Unscrew the captive screw located at the bottom of the HTS support bracket. NOTE There is no captive screw in BD LSRFortessa, because the HTS is placed on a sliding drawer. Pull the HTS out (about 2 inches) from the support bracket.
  • Page 19 Captive screw HTS positioned on its support bracket Chapter 2: HTS Removal and Reinstallation...
  • Page 20 Figure 2-1 Electrical connection to the HTS Power plug Serial cable HTS Customer Care Kits User’s Guide...

  • Page 21: Reinstalling The Hts

    Figure 2-2 Fluidic connections to HTS Routing of power and communication cables Waste fluidic connection Sheath fluid connection Reinstalling the HTS Place the HTS on the support bracket. Reconnect the power cable and the serial communications cable. Reconnect the sheath and waste connectors to the HTS. Chapter 2: HTS Removal and Reinstallation...
  • Page 22 Place the cover back on the HTS and turn it on. Attach the sample coupler to the SIT. Turn on the cytometer. Start BD FACSDiva™ software or Plate manager software if connected to the BD FACS Calibur and perform an HTS prime. HTS Customer Care Kits User’s Guide...

  • Page 23: Chapter 3: Wash Station Assembly Replacement

    Wash Station Assembly Replacement Perform the wash station assembly maintenance tasks every six months or when the following symptoms occur: • The HTS exhibits higher than normal carryover between samples • The wash station overflows Required Kit Components The sample injection tubing (3) connects the injection port/wash station (1) to the secondary pump valve (not shown).

  • Page 24: Replacing Wash Station Components

    Injection port/wash station Replacing Wash Station Components Perform the monthly cleaning procedure to decontaminate the sample injection port/wash station. For instructions, see your BD High Throughput Sampler User’s Guide. Turn off the cytometer and HTS if needed. Remove the HTS according to procedure in Chapter 2.
  • Page 25 Tubing assembly Secondary pump The tubing nuts are normally finger tight. If it is difficult to unscrew a tubing nut, use a 5/16 wrench to loosen it. Turn the sample injection port (see figure) slightly to disengage the magnets, and lift the port approximately 1 inch. Disconnect the silicon tubing from the sample injection port.

  • Page 26: Assembling The Sample Injection Tubing Assembly

    Secure the silicon tubing so that it does not slide back into the hole, and carefully remove the sample injection port assembly. Assembling the Sample Injection Tubing Assembly To prevent the cross-threading of fittings, ensure that mating fittings are aligned to one another before screwing them together. All fittings should be secured only finger tight and not beyond.
  • Page 27 Install and tighten the sample injection tubing assembly to the top of the secondary pump and install the HTS on the cytometer (see Chapter 2). Chapter 3: Wash Station Assembly Replacement...
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  • Page 29: Chapter 4: Tubing Assembly Replacement

    Tubing Assembly Replacement Perform the tubing assembly from probe to the primary pump maintenance tasks when the following symptoms occur: • Tubing is clogged • The systems displays a primary pump (Pump-1) error • No events are detected Required Kit Components Item Part Number Description...

  • Page 30: Replacing Tubing Assembly Components

    Clamp Tubing assembly Primary pump NOTE Use caution when handling the sample injection port assembly. This assembly is fragile and can be easily damaged. Replacing Tubing Assembly Components Turn off the cytometer and HTS and move the sample probe into the sample injection port.
  • Page 31 Remove the sample probe tubing by rotating the tubing clamp on the arm to allow enough clearance for the tubing to be removed. If a connector is difficult to remove due to saline deposits, clean it with DI water. If it still does not unscrew, then carefully use the 5/16 wrench and gently loosen the clamp.
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  • Page 33: Chapter 5: Sample Coupler And Tubing Replacement

    Sample Coupler and Tubing Replacement The sample coupler is frequently installed or removed from the SIT to run the cytometer in tube acquisition mode. The sample coupler tubing is attached to the HTS secondary pump. Replace the sample coupler tubing annually or when the following symptoms occur: •...

  • Page 34: Bd Facscanto And Facscanto Ii Systems

    Description 339340 Sample coupler, secondary pump to SIT Figure 5-1 Sample coupler and tubing for the BD FACSCanto and BD FACSCanto II systems Sample coupler, secondary pump to SIT Secondary pump Turn off the cytometer, and remove the HTS from the cytometer (see Chapter 2).
  • Page 35 Secondary pump valve Install the new sample coupler tubing provided in the HTS Customer Care Kit. Connect the new tubing to the left port in the secondary pump valve, and tighten the tubing until the fitting is finger tight. NOTE Valves contain sealing washers in each port. If you over-tighten the fitting, the sealing washers can be compressed, resulting in a blocked port.

  • Page 36: Bd Facscalibur, Bd Lsr Ii, Or Bd Lsrfortessa Systems

    BD FACSCalibur, BD LSR II, or BD LSRFortessa Systems Item number Part number Description 335452 Sample coupler, secondary pump to SIT Sample coupler, secondary pump to SIT Secondary pump Fill a small, clean container with approximately 300 mL of DI water.

  • Page 37: Chapter 6: Pump Syringe Replacement

    Pump Syringe Replacement Glass syringes are used in the primary and secondary pumps of the HTS unit. For reference, the syringe associated with the secondary pump is shown the following figure. Sharp raised edge Syringe Narrow up 1/4 - 28 thread Syringe barrel Perform the replacement of the pump syringe maintenance tasks annually or when the following symptoms occur:...

  • Page 38: Required Kit Components

    If you are replacing the secondary pump, remove the HTS (see Chapter 2), then use the Replacing the Syringe procedures. Removing Liquid from the Syringe (For Primary Pump Syringe) Verify that the cytometer and BD FACSDiva software are running. From the Acquisition Dashboard select RUN PLATE. Remove the liquid from the syringe: Disconnect the sheath connector from the back of the HTS.

  • Page 39: Replacing The Syringe

    Replacing the Syringe Loosen the plunger lock screw (indicated in the following figure) approximately three full turns. Valve Syringe barrel Plunger lock screw Lower the plunger drive by pushing the plunger lock screw down. Unscrew the syringe barrel and remove it from the lower part of the valve. Grasp the metal ring at the top of the syringe and turn counterclockwise.
  • Page 40 Tighten the plunger lock screw, making sure it is securely tightened. Plunger Lubricant Plunger holder Apply a generous amount of O-ring lubricant to the plunger lock screw to prevent seizing in the future. You can apply the lubricant in the lock lever hole before placing the plunger in it.
  • Page 41 Turn on the cytometer and using the BD FACSDiva software, perform a Prime. Secondary pump Syringe Chapter 6: Pump Syringe Replacement...
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  • Page 43: Chapter 7: Fluidic Connection And In-Line Fluidic Filter Replacement

    Fluidic Connection and In-line Fluidic Filter Replacement This chapter covers the replacement of the two fluidic connections between the cytometer and the HTS. These two fluidic connections are sheath and waste connections. Perform the fluidic connections maintenance and replace the sheath filter: •...

  • Page 44: Required Kit Components

    Coupling insert, hose barb (blue) 59-10090-05 Coupling insert, hose barb (orange) (waste) 343529 Coupling insert, hose barb (white) Figure 7-1 Filter Filter Figure 7-2 BD FACSCanto II connectors Blue hose barb Orange hose barb Filter White hose barb HTS Customer Care Kits User’s Guide...

  • Page 45: Replacing The Filters

    Replacing the Filters Turn off and remove the HTS from the cytometer (see Chapter 2). Replace the two liquid connections between the cytometer and the HTS: Move the HTS away from the cytometer to provide access behind the HTS. Disconnect the quick-connector at the cytometer interface panel and the back of the HTS (orange quick-connector end in Canto II).
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  • Page 47: Chapter 8: Probe And Probe Tubing/Body Assembly Replacement

    Probe and Probe Tubing/Body Assembly Replacement The probe is located at the end of the probe assembly arm. Replace the probe along with the probe tubing or body assembly annually, or when the following occurs: • Probe is bent or is not taking up sample •...

  • Page 48: Replacing The Probe And Probe Tubing/Body Assembly

    Turn off the cytometer and remove the HTS cover. NOTE On the BD FACSCanto, BD LSR II, and BD FACS Calibur systems, you will need to turn off the power on the cytometer and the HTS separately.
  • Page 49 Tighten the probe nut at the top of the probe and attach the tubing connector to the probe. Turn on the cytometer and HTS if needed. Start BD FACSDiva software and perform an HTS prime. Chapter 8: Probe and Probe Tubing/Body Assembly Replacement...
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  • Page 51: Chapter 9: Cleaning/Shutdown Procedure

    Cleaning/Shutdown Procedure The BD FACSCalibur, BD LSR II, and BD LSRFortessa do not have a Fluidic Shutdown cycle that automatically replaces BD FACS™ sheath solution with BD FACS shutdown solution or DI water. These procedures are for the BD FACSCalibur, BD LSR II, Special Order BD LSR II, and Special Order BD LSRFortessa systems as they do not have a fluidic shutdown cycle built into the software.
  • Page 52 Submerge the other end of the purge line into the DI water. Using BD FACSDiva software, perform a system prime. Repeat 3 times. For BD FACS Calibur, use BD Plate Manager software to run the prime. Remove the purge tubing.

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