Consumables; Use Of The Perfect Fit Frame With Single Tubes And Strip Tubes - Agilent Technologies Mx3000P Setup And User Manual

Consumables

Use of the Perfect Fit Frame with Single Tubes and Strip Tubes

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The thermal block of the Mx3000P/Mx3005P system is designed to
accommodate a variety of 96-well PCR plates, 200-µl PCR strip tubes, or
individual 200-µl PCR tubes that have separate, optically clear caps. For
best results, use the following Agilent consumables:
96-well PCR plates, 0.2-ml, non-skirted, Catalog #401333
96-well PCR plates, 0.2-ml, semi-skirted, Catalog #401334
8× strip tubes, 0.2-ml, Catalog #401428
8× optical strip caps, Catalog #401425
Single PCR reaction tubes (without caps), 0.2-ml, Catalog #410023
Prior to using a new type of plasticware for a run, test the fit in the thermal
block, and attempt to close the heated lid. If you encounter resistance when
closing the lid, do not use the plasticware for Mx3000P/Mx3005P system
runs. Using plates or tubes that are too tall for the thermal block or using
domed sample caps may prevent the heated lid from closing properly and
may damage the instrument scanner.
Use only non-skirted or semi-skirted plates with the Mx3000P and
Mx3005P systems. Full-skirted plates cannot be properly seated in the
thermal block.
Important
When using individual tubes or strip tubes as sample
containers, insert the Perfect Fit frame on the thermal block
platform before loading the samples. See Use of the Perfect
Fit Frame with Single Tubes and Strip Tubes for more
information.
Use of tubes that are not designed for the Mx3000P and
Mx3005P instruments can result in poor experimental results
or damage to the instrument. The Agilent consumables for use
in the Mx4000 instrument are not compatible for use in the
Mx3000P or Mx3005P systems, and can result in difficulty
closing the heated lid and damage to the heated lid.
Prior to performing a run using individual tube or strip tube sample
containers, install the Perfect Fit frame on the thermal block platform. The
Perfect Fit frame optimizes the contact between the sample tubes and the
heated lid. One Perfect Fit frame is supplied with the Mx3000P/Mx3005P
System, and additional frames are available separately (Agilent Catalog
#401421). See Figure 3 for an illustration of the Perfect Fit frame and
Figure 4 for the appearance of the frame when installed correctly on the
thermal block.
Mx3000P and Mx3005P QPCR Systems Setup and User's Guide