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Pellicon
2 and 3 Mini Holder
User Guide

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Summary of Contents for Millipore Pellicon 2

  • Page 1 ® Pellicon 2 and 3 Mini Holder User Guide...
  • Page 2 This manual is believed to be complete and accurate at the time of publication. In no event shall Millipore Corporation be liable for incidental or consequential damages in connection with or...
  • Page 3: Table Of Contents

    Pellicon-2 and 3 Mini Holder User Guide Contents Unpacking ....................5 Plumbing and Instrumentation .............. 6 Assembling the Pellicon 2 and 3 Mini Holder ........7 Feed and Retentate Fitting Connections ........ 7 Permeate Fitting Connections ..........7 Connecting Pressure Gauge Adapter Fittings to FEED and RETENTATE Fittings .........
  • Page 4 www.millipore.com...
  • Page 5: Unpacking

    Pellicon-2 and 3 Mini Holder User Guide Unpacking The Pellicon 2 and 3 Mini Holder comes packed in a box and includes the following items: Description Quantity Manifold with two tie rods, attached to a base End plate Tie rod spacer...
  • Page 6: Plumbing And Instrumentation

    Plumbing and Instrumentation The concentration mode is the most commonly used mode for the concentration of proteins and viruses and is shown in Figure 2 without the diafiltrate. During this mode, material retained by the membrane (retentate) flows out of the retentate fitting and is recirculated to the original product container.
  • Page 7: Assembling The Pellicon 2 And 3 Mini Holder

    Pellicon-2 and 3 Mini Holder User Guide Assembling the Pellicon 2 and 3 Mini Holder Feed and Retentate Fitting Connections Configurations for connecting fittings to the FEED and the RETENTATE fittings, shown in Figure 3, include: Connecting a pressure and sanitary diaphragm valve to the RETENTATE fitting, and a pressure gauge to the FEED fitting.
  • Page 8: Connecting Pressure Gauge Adapter Fittings To Feed And Retentate Fittings

    Connecting Pressure Gauge Adapter Fittings to FEED and RETENTATE Fittings The Pellicon 2 and 3 Mini Holder Pressure Gauge Adapter Fittings are short-leg tees to keep the working fluid volume low and avoid non-sanitary low-flow areas in the piping. Two ¾-inch sanitary gaskets and two sanitary fitting clamps are supplied with each pressure gauge adapter fitting.
  • Page 9: Connecting The Pressure Gauge

    Clamping the Pressure Gauge Adapter Fittings to the Mini Holder Fitting Connecting the Pressure Gauge Analog and digital pressure gauges are available from Millipore. Seat the ¾-inch sanitary gasket in the groove on the pressure gauge adapter fittings. Hold the gasket in place and press the pressure gauge diaphragm against the ¾-inch side branch of the pressure gauge adapter fittings, as shown in Figure 7.
  • Page 10: Connect The Permeate Fittings

    Holding the manifold with one hand, slide the filter gasket (Pellicon 2 Cassettes only) tabs over the tie rods and press the filter gasket against the manifold, as shown in Figure 8.
  • Page 11: Attaching The End Plate

    Holding the manifold with one hand, slide the filter gasket (Pellicon 2 Cassettes only) tabs over the tie rods and press the filter gasket (Pellicon 2 Cassettes only) against the filter, as shown in Figure 8.
  • Page 12 Attach the deep socket to the torque wrench by firmly pressing the deep socket onto the torque wrench drive. Attaching the deep socket may require force. Hand tighten the nuts evenly by alternating from one nut to the other.
  • Page 13: Setup For Autoclaving

    Do not autoclave with the holder in an upright position, because the feet will compress and become loose. Autoclave the Pellicon 2 and 3 Mini Holder at 121º C to 125º C for 30 minutes per cycle.
  • Page 14: Ordering Information

    Ordering Information Description Catalogue Number Pellicon 2 and 3 Mini Holder Includes holder, 4 sanitary gaskets, 4 sanitary clamp fi ttings, and XX42PMINI assembly instructions Spare Parts and Accessories Description Catalogue Number (per kit) Kits for short tie rods including 2 tie rods...
  • Page 15: General Limited Warranty

    In the event of a breach of the foregoing warranty, Millipore’s sole obligation shall be to repair or replace, at its option, the applicable product or part thereof, provided the customer notifies Millipore promptly of any such breach.
  • Page 16: Technical Assistance

    You can also visit the tech service page on our web site at http://www.millipore.com/techservice. Millipore and Pellicon are registered trademarks of Millipore Corporation. The M logo is a trademark of Millipore Corporation. Copyright © 1994, 2009 Millipore Corporation. All rights reserved.

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Pellicon 2 mini holder

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