Time-Of-Flight Signal; Amplification; Time-Of-Flight Pulses - Beckman Coulter Navios Instructions For Use Manual

Time-Of-Flight Signal

Time-of-Flight is the transit time of a cell or particle to traverse the laser beam. The pulse width of
a peak pulse is measured and can be assigned to any peak signal. An application for Time-of-Flight
is doublet discrimination.
Figure 3.9 Time-Of-Flight Pulses
b
c
1. Pulse height/shape

Amplification

Some voltage pulses must be amplified so that the characteristics of the cells can be measured. The
system lets you:
• Increase the gain to linearly amplify the integral, peak and Time-of-Flight signals.
• Logarithmically transform the linear intregral and peak signal data.
A logarithmic transformation accentuates the differences between the smaller pulses and reduces
the differences between the larger pulses.
773232AH
2C 4C
2. Time
2C + 2C = 4C
Operation Principles 3
Signal Processing
3-9