Voltage Pulse Formation, Peak Signal - Beckman Coulter Navios Instructions For Use Manual

• Part II of
and therefore, the pulse height, reaches a maximum.
• Part III of
The intensity of light scatter or fluorescence determines the height of the peak pulse (see
Figure 3.7). The time the particle is in the laser beam determines the width of the pulse. Therefore,
the total fluorescence (intensity and time) determines the area under the pulse.
how three cells with the same amount of total fluorescence but with different fluorescence
intensities, produce different peak pulses.
Figure 3.7 Voltage Pulse Formation, Peak Signal
I
II
b
III
b
1. Laser beam
2. Cell/Particle
3. Volts
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Figure 3.7 shows when the cell is in the center of the laser beam and the scattered light,
Figure 3.7 shows when the cell leaves the laser beam and the scattered light decreases.
b
c
c
c
4. Time
5. Pulse height
d
e
d
e
d
f
e
Operation Principles 3
Signal Processing
Figure 3.8 shows
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