Shimadzu LC-10 User Manual
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Shimadzu LC-10 User Manual

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Clarity Controls
Shimadzu LC-10/20 System
LC + DET + AS + CO
ENG
Code/Rev.: M108/70B
Date: 11/30/2016
Phone: +420 251 013 400
DataApex Ltd.
Fax: +420 251 013 401
Petrzilkova 2583/13
clarity@dataapex.com
158 00 Prague 5
www.dataapex.com
The Czech Republic

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  • Page 1 Clarity Controls Shimadzu LC-10/20 System LC + DET + AS + CO Code/Rev.: M108/70B Date: 11/30/2016 Phone: +420 251 013 400 DataApex Ltd. Fax: +420 251 013 401 Petrzilkova 2583/13 clarity@dataapex.com 158 00 Prague 5 www.dataapex.com The Czech Republic...
  • Page 2 Clarity ® , DataApex ® and ® are trademarks of DataApex Ltd. Microsoft ® and Windows TM are trademarks of Microsoft Corporation. DataApex reserves the right to make changes to manuals without prior notice. Updated manuals can be downloaded from www.dataapex.com. Author: DM...

  • Page 3: Table Of Contents

    4.3.4 Method Setup - AS - Pretreatment 4.3.5 Device Monitor - Shimadzu LC-10/20 System 4.3.6 Report Setup 4.4 PDA 4.4.1 Shimadzu LC-10/20 System Setup - PDA Detector 4.4.2 Method Setup - PDA 4.4.3 Method Setup - Acquisition 4.4.4 Device Monitor 4.4.5 Report Setup...
  • Page 4 Table of Contents Clarity Controls 4.5.1 Shimadzu LC-10/20 System Setup - Thermostat 4.5.2 Method Setup - Thermostat - Thermostat 4.5.3 Method Setup - Thermostat - Time Program 4.5.4 Device Monitor 4.5.5 Report Setup 5 Troubleshooting 6 Vial Plate Numbers - ii -...
  • Page 5 Shimadzu LC-10/20 System Table of Contents To facilitate the orientation in the Shimadzu LC- 10/20 System manual and Clarity chromatography station, different fonts are used throughout the manual. Meanings of these fonts are: Instrument (blue text) marks the name of the window to which the text refers.

  • Page 7: Shimadzu Lc-10/20 System

    Shimadzu LC-10/20 System 1 Shimadzu LC-10/20 System 1 Shimadzu LC-10/20 System This manual describes the setting of the Shimadzu LC-10/20 System . The control module enables direct control of the instrument over serial line or LAN (in case of LC-20 series).
  • Page 8 1 Shimadzu LC-10/20 System Clarity Controls Thermostats: CTO- 10A, CTO- 10AC, CTO- 10Avp, CTO- 10ACvp, CTO- 10ASvp, CTO-20A*, CTO-20AC* The list continues to extend, for up to date list see the website www.dataapex.com. Some devices also require a firmware of particular minimal version for proper communication with Clarity (for more details see the chapter Minimal version of device firmware required on pg 4).

  • Page 9: Requirements

    Detector will need the PDA extension (p/n A29) to operate. Supported Shimadzu system controller (see the full list of supported controllers in the chapter "Shimadzu LC-10/20 System" on pg 1.). The controller is needed even for the control of standalone PDA detector.

  • Page 10: Minimal Version Of Device Firmware Required

    2 Requirements Clarity Controls 2.1 Minimal version of device firmware required Tab 1: Controllers : Name of Device: Minimal version of firmware required: SCL-10Avp 5.33 Subcontroller 5.20 OptionBox-L Tab 2: Pumps : Name of Device: Minimal version of firmware required: LC-10ADvp 5.26 LC-10ATvp...

  • Page 11: Installation Procedure

    Level: Enhanced Caution: Only COM ports up to COM9 are supported. Serial ports with higher numbers cannot be used. This also means that the Shimadzu LC-10/20 System cannot be connected to the serial port on Net- PAD (COM101 and COM102).
  • Page 12 Clarity Controls LC-20 components with SCL-10Avp controller Shimadzu LC- 20 components have to be set to the LC- 10 emulation mode when they should be working under the LC-10 controller. This is done from the device's keyboard according to this procedure (procedure...

  • Page 13: Software Installation

    Select the target directory and press the Next button. Select Custom installation. The control module for the Shimadzu LC- 10/20 System is automatically included in it. If the PDA detector is to be used, select the Detector Control option (its icon is checked, but grayed) and either click the checkbox to change it to checked (not grayed) state or press the Details…...

  • Page 14: Clarity Configuration

    3 Installation Procedure Clarity Controls 3.3 Clarity Configuration Caution: If the PDA detector will be used, it must be switched on prior to starting the computer. Fig 3: System Configuration Start the Clarity station by clicking on the icon on the desktop. Invoke the System Configuration dialog accessible from the...
  • Page 15 ② You can specify the searching filter to simplify the finding of the driver. ③ Select the LC-10/20 System item and press the Add button. Shimadzu LC-10/20 System Setup dialog will appear. Fig 4: Shimadzu LC-10/20 System Setup Select the type of the controller in the Model field and the Serial Port or IP Address to which the controller is connected.
  • Page 16 Clarity Controls Note: When you only change some of the already configured parts of the Shimadzu LC-10/20 System, it is recommended to remove the system from the given Instrument tab and add it there once more. ⑧ Set the Ext. Start Dig. Input and Ready Dig. Output numbers for your acquisition card according to the wires being used for synchronization.

  • Page 17: Shimadzu Lc-10/20 System Setup

    Pressing the Change button opens the Digital Input Names dialog which allows to set the custom name for the virtual Shimadzu LC-10/20 System digital input. This input allows to start Clarity run by the signal of the Shimadzu LC-10/20 System controller.

  • Page 18: Shimadzu Lc-10/20 System Setup - Setup

    LC- 10 emulation mode (for more details see the chapter Installation Procedure on pg 5). Serial Port Selects the serial port to which the Shimadzu LC-10/20 System is connected. IP Address Sets the IP Address if using the LAN connection. LAN can only be used for the Shimadzu LC-20 controllers.
  • Page 19 Fig 7: Hardware Configuration Pumps Sets the model of the pump connected. Up to three pumps may be configured in a Shimadzu LC-10 and four pumps on Shimadzu LC-20 System. The first pump may have valves configured, other pumps are not allowed to have any valves.

  • Page 20: Shimadzu Lc-10/20 System Setup - Controller

    3 Installation Procedure Clarity Controls 3.4.2 Shimadzu LC-10/20 System Setup - Controller Fig 8: Shimadzu LC-10/20 System Setup - Controller Switches System Protect While checked, in case of exceeding the maximal pressure the flow rate will be decreased to one half of the set value. If unchecked, the exceeding will shut-off the pump.
  • Page 21 Shimadzu LC-10/20 System 3 Installation Procedure External Start This control sets the conditions for closing the controllers start relays at the beginning of the analysis. Possible options are: Disable - External start is disabled. All Runs - Closes start relays at each analysis start including starts with no injection.

  • Page 22: Using The Control Module

    Shimadzu LC-10/20 System Setup dialog. These new tabs enable the setting of the Shimadzu system operation program. Note: The instrument method is always sent to the Shimadzu LC- 10/20 System as a whole.

  • Page 23: Pump

    Shimadzu LC-10/20 System Setup dialog allows to set the parameters of the given pump. Fig 9: Shimadzu LC-10/20 System Setup - Pump Auxiliary Pump Check for the pump to become auxiliary pump (not part of the Gradient Table). If there are more pumps added in the configuration and the first one is set either as low- pressure gradient or binary pump, the other pumps are automatically set as Auxiliary pumps.
  • Page 24 Shimadzu LC-10/20 System 4 Using the control module High Pressure Limit Upper pressure limit. Number entered will be sent to the pump and checked by the pump firmware. Note: Exceeding of High Pressure Limit or Low Pressure Limit defined here will cause a system error, requiring the closure and reopening of the given Instrument.

  • Page 25: Method Setup - Lc Gradient

    4 Using the control module Clarity Controls 4.1.2 Method Setup - LC Gradient Method Setup - LC Gradient dialog serves for setting up the LC instrument method. Fig 10: Method Setup - LC Gradient Gradient Table A table for setting the composition of the mobile phase and the overall flow rate as a function of time.
  • Page 26 Note: The values of the Flow for the Shimadzu LC-10/20 pumps vary in certain range according to the type of the pump. Check the device manual for valid Flow ranges for your pump.

  • Page 27: Gradient Options

    During this delay the pressure in chromatographic system can stabilize. In addition to those limits, a pressure limits are set in the Shimadzu LC- 10/20 System Setup - Pump dialog. Those limits are checked in the pump firmware.
  • Page 28 Note: Min. Pressure and Max. Pressure for the Shimadzu LC- 10/20 pumps vary in certain range according to the type of the pump and valves used. Check the device manual for valid values for your pump.

  • Page 29: Method Setup - Lc

    LC Status Invokes the hardware configuration dialog listing the settings of the Shimadzu LC-10/20 System . The upper part shows the settings of the controller, the lower part the settings of other modules. Fig 12: Method Setup - LC - Hardware Configuration...
  • Page 30 Shimadzu LC-10/20 System 4 Using the control module Auxiliary Pump tab Fig 13: Method Setup - LC - Auxiliary Pump Initial Flow Sets the initial flow of the auxiliary pump. Solvent B Concentration Allows to set the percentage of the second solvent in the mixture created by auxiliary binary pump.
  • Page 31 4 Using the control module Clarity Controls LC Valve tab Tab listing the Valve Position table. Each row defines the change of the valve position in given time. These settings can be later printed as a part of the report. Note: The valves set on particular pumps may not be governed from the Method...

  • Page 32: Method Setup - Advanced

    4.1.4 Method Setup - Advanced Fig 15: Method Setup - Advanced Shimadzu LC- 10/20 System provides the pump flow and pressure auxiliary signals for use in Clarity. To save the auxiliary signals into each chromatogram measured according to the given method, check the...

  • Page 33: Device Monitor

    4 Using the control module Clarity Controls 4.1.5 Device Monitor The pump status dialog can be invoked by the Monitor - Device Monitor command from the Instrument window or using the LC Monitor icon. Device Monitor - LC Monitor It displays the actual flows of particular solvents, as well as the total flow, the total pressure and the analysis time.
  • Page 34 Shimadzu LC-10/20 System 4 Using the control module Device Monitor - Auxiliary Pump Each Auxiliary pump has its own device monitor displaying the actual flow and pressure on the device. Fig 18: Device Monitor - Auxiliary pump - 28 -...

  • Page 35: Report Setup

    4 Using the control module Clarity Controls 4.1.6 Report Setup All of the pump settings accessible on the Method Setup - LC Gradient and in the Gradient Options dialog are reported, if the pump is configured as the part of the gradient. To do so, the Instrument Control parameter on Method tab of the Report Setup...

  • Page 36: Detector

    Shimadzu LC-10/20 System 4 Using the control module 4.2 Detector 4.2.1 Shimadzu LC-10/20 System Setup - Detector Shimadzu LC-10/20 System Setup - Detector tab for SPD detectors and RF detectors looks the same, although some items may be missing depending on the type of the detector.
  • Page 37 All options are the same except for the Large Scale Preparative Option Installed. Fig 22: Shimadzu LC-10/20 Detector setup - RID-10A Large Scale Preparative Option Installed Defines whether the optional Large Scale Preparative Option is installed in the RID-10A detector.

  • Page 38: Method Setup - Acquisition - Detector

    Shimadzu LC-10/20 System 4 Using the control module 4.2.2 Method Setup - Acquisition - Detector In case of greater number of detectors configured on one Instrument, it is possible to switch to the desired detector by selecting it in the Select...
  • Page 39 Sampling Rate Sets the sampling rate of the detector. The available detector sample rates are dependent on the Base Period settings on the Shimadzu LC-10/20 System Setup - Detector tab - by using the shortest Base Period of 20 ms, the Sampling Rate can be set up to 50 Hz.

  • Page 40: Method Setup - Acquisition - Rf Detector

    Shimadzu LC-10/20 System 4 Using the control module 4.2.2.1 Method Setup - Acquisition - RF Detector This sub-tab replaces the Detector sub-tab when one of the configured RF-10A and RF-10Axl detectors is selected in the Select Detector field. Fig 24: Method Setup - Acquisition - RF Detector Excitation Wavelength Sets the excitation wavelength.
  • Page 41 4 Using the control module Clarity Controls System Setup - Detector tab - by using the shortest Base Period of 20 ms, the Sampling Rate can be set up to 50 Hz. Please note that in dual mode the Base Period is limited to 100 ms and maximum Sampling Rate to 10 - 35 -...

  • Page 42: Method Setup - Acquisition - Rid Detector

    This field specifies the operating mode of the detector. The contents of the field depend on the state of the Large Scale Preparative Option Installed checkbox in the Shimadzu LC-10/20 System Setup - Detector dialog for RID detector. If the checkbox is checked, the Large Scale Preparative text is the only option to be selected in the field.
  • Page 43 4 Using the control module Clarity Controls Cell Temperature Sets the temperature of the detector cell. Check Cell Temperature When checked, the Instrument will not be ready until the actual temperature will reach the set value (with the 1.5 °C tolerance). While the checkbox is unchecked, no temperature verification will be performed.

  • Page 44: Method Setup - Acquisition - Time Program

    Shimadzu LC-10/20 System 4 Using the control module 4.2.3 Method Setup - Acquisition - Time Program This sub-tab allows to reset the detector parameters defined on other tabs based on the analysis time. Note that the options listed in the Event column depend on the type of the detector (not all events listed under the picture are accessible).
  • Page 45 4 Using the control module Clarity Controls Gain - Change the current Gain parameter. Only available for RF detectors. Excitation Wavelength - Change the current Excitation Wavelength parameter. Only available for RF detectors. Emission Wavelength - Change the current Emission Wavelength parameter.

  • Page 46: Method Setup - Acquisition - Analog Output

    Shimadzu LC-10/20 System 4 Using the control module 4.2.4 Method Setup - Acquisition - Analog Output Fig 27: Method Setup - Acquisition - Analog Output Auxiliary Range Sets output range of integrator terminals. Caution: Auxiliary range will affect the acquired signal. For RID-10A and RID-20A detectors use 0.25 mRIU/V to get correct signal values...

  • Page 47: Device Monitor

    4 Using the control module Clarity Controls 4.2.5 Device Monitor Device Monitor window can be invoked by the Monitor - Device Monitor command from the Instrument window or using the Device Monitor icon. It displays the actual wavelength and the lamp used, the cell temperature (if configured) and allows to perform the Autozero action and switch the signal on outside of the analysis.
  • Page 48 Shimadzu LC-10/20 System 4 Using the control module Device Monitor - RID Detector The RID detectors has slightly different Device Monitor window with other options on the monitor pane. Only new items are described: Fig 29: Device Monitor - RID Detector Balance When invoked, sends the Balance command to the detector.

  • Page 49: Report Setup

    Report Setup dialog is checked. For each detector set in the Shimadyu LC-10/20 System Setup dialog, a specific section of the report will be printed. Fig 31: Report Setup Two groups of parametres are then printed, one common to all channels of a given detector, the other specific to particular channels.
  • Page 50 Shimadzu LC-10/20 System 4 Using the control module The report setup of the RID- 10A detector differs only in the items displayed, it is the same in all other respects. Report Setup - RF Detector Fig 33: Report Setup - RF Detector The report setup of the RF detectors differ only in the items displayed, it is the same in all other respects.

  • Page 51: Autosampler

    4 Using the control module Clarity Controls 4.3 Autosampler The autosamplers used with the Shimadzu LC-10/20 System allow for automated injection of samples. This can only be performed from the Sequence window, not from the Single Analysis dialog (where it is impossible to specify from which position the injection will be performed).
  • Page 52 Shimadzu LC-10/20 System 4 Using the control module MTP Sample Order Defines the numbering of wells in the plate when the MTP Tray is used. If other trays are used, this option is ignored. Possible parameters are Horizontal or Vertical.

  • Page 53: Method Setup - As - Sampler

    4 Using the control module Clarity Controls 4.3.2 Method Setup - AS - Sampler Fig 35: Method Setup - AS - Sampler Rack Type Defines the sample rack type. For some trays, it is also necessary to choose the correct Tray Type (Single or Dual). Detect Auto detects the Rack Type and sets the Needle Stroke parameter.
  • Page 54 Sets the needle depth used when aspirating from the Control Rack (used with the SIL-20 samplers). Rinse Pump Section enabled by checking the Rinse Pump Installed option in the Shimadzu LC-10/20 System Setup - Autosampler dialog. Rinse Method Sets the method for the Rinse Pump . Port Only, Pump Only, Pump Then Port, Port Then Pump, Pump And Port Between Analyses and Auxiliary 1 (2) methods are available.

  • Page 55: Method Setup - As - Sampler (Sil-10Axl)

    4 Using the control module Clarity Controls 4.3.2.1 Method Setup - AS - Sampler (SIL-10Axl) This sub-tab replaces the Sampler sub-tab when the SIL-10Axl sampler is configured on the Instrument. The majority of items in the dialog is the same, the differing items are described below: Fig 36: Method Setup - AS - Sampler (SIL-10Axl) Caution: Due to the error in the MIMIC Libraries, SIL- 10AXL sampler does not...

  • Page 56: Method Setup - As - Sampler (Sil-10Af)

    Shimadzu LC-10/20 System 4 Using the control module 4.3.2.2 Method Setup - AS - Sampler (SIL-10AF) This sub-tab replaces the Sampler sub-tab when the SIL-10AF sampler is configured on the Instrument. The majority of items in the dialog is the...

  • Page 57: Method Setup - As - Time Program

    4 Using the control module Clarity Controls 4.3.3 Method Setup - AS - Time Program Fig 38: Method Setup - AS - Time Program Time Table It is possible to set events to be performed during the analysis run based on the analysis time, namely the Inject, Rinse and Start Pretreatment actions.

  • Page 58: Method Setup - As - Pretreatment

    Shimadzu LC-10/20 System 4 Using the control module 4.3.4 Method Setup - AS - Pretreatment The logic for all calculations of pretreatment vials goes as follows: The vial specified in the Sequence window for the injection (called the Sample vial , SV) needs to be empty when performing pretreatment. The actual...
  • Page 59 4 Using the control module Clarity Controls Note: The Advanced pretreatment mode is not currently supported by Clarity. The set of other parameters available differ according to the Mode selected. Dilution Source Vial Specifies the source vial for the original sample (before dilution). Note: The vial used for dilution and injection is specified in the Sequence...
  • Page 60 Shimadzu LC-10/20 System 4 Using the control module Reagent Fig 40: Method Setup - AS - Pretreatment (Reagent) Part of the fields (namely Mixing Count, Mixing Volume , Bubbling and Wait Time) in the Reagent mode has the same meaning as in the Dilution mode.
  • Page 61 4 Using the control module Clarity Controls Inner Needle Wash Fig 41: Method Setup - AS - Pretreatment (Inner Needle Wash) Pre-Rinse Sets the rinsing prior to the injection (which is not recommended). The same options as for the Rinse operation (see below) apply. Rinse 1[2] Sets the first (second) rinse solvent.

  • Page 62: Device Monitor - Shimadzu Lc-10/20 System

    Shimadzu LC-10/20 System 4 Using the control module 4.3.5 Device Monitor - Shimadzu LC-10/20 System Device Monitor window can be invoked by the Monitor - Device Monitor command from the Instrument window or using the Device Monitor icon. It displays the actual cooler temperature (if configured) and allows to perform the Purge and Rinse actions.

  • Page 63: Report Setup

    4.3.6 Report Setup The autosampler settings accessible from the Method Setup - AS tab and Shimadzu LC- 10/20 System Setup - Autosampler dialog may also be included in the report. To do so, the Injection Control parameter on the Method...

  • Page 64: Pda

    Uniquely identifies the SCSI adapter board. This number is assigned by the operation system and can be found in the Device Manager. The default value is 0. See Shimadzu SPD-M10Avp manual for more details. Device ID Number of the PDA Detector unit (set on the back of the unit). The default value is 4.
  • Page 65 4 Using the control module Clarity Controls Signal 1 (..4) Name Defines the names of the particular detector signals. - 59 -...

  • Page 66: Method Setup - Pda

    Shimadzu LC-10/20 System 4 Using the control module 4.4.2 Method Setup - PDA Fig 45: Method Setup - PDA Start Wavelength Defines the lowest value of the wavelength to be used for the acquisition of spectra. This value also influences the lowest possible value of the...
  • Page 67 4 Using the control module Clarity Controls takes to gather one spectrum (based on the Sampling Rate parameter). Slit Width Defines the wavelength accuracy provided by the hardware. Cell Temperature Sets the temperature of the detector cell. Check Cell Temperature When checked, the Instrument will not be ready until the actual temperature will reach the set value (with the 1.5 °C tolerance).

  • Page 68: Method Setup - Acquisition

    Shimadzu LC-10/20 System 4 Using the control module 4.4.3 Method Setup - Acquisition Fig 46: Method Setup - Acquisition - PDA Signal Wavelength Sets the wavelength of the desired analog signal. This value must lie in the range set by the Start Wavelength and End Wavelength parameters...

  • Page 69: Device Monitor

    4 Using the control module Clarity Controls 4.4.4 Device Monitor Device Monitor window can be invoked by the Monitor - Device Monitor command from the Instrument window or using the Device Monitor icon. It allows to Autozero the detector, turn its lamp(s) on or off and start the data monitoring outside of the analysis run.

  • Page 70: Report Setup

    Shimadzu LC-10/20 System 4 Using the control module 4.4.5 Report Setup All PDA detector settings accessible on the Shimadzu LC-10/20 System Setup - PDA tab, in the Method Setup - Acquisition tab for the PDA signals and settings from the...

  • Page 71: Thermostat

    4.5.1 Shimadzu LC-10/20 System Setup - Thermostat Fig 49: Shimadzu LC-10/20 System Setup - Thermostat Some options may be missing or shaded depending on the type of the thermostat installed.

  • Page 72: Method Setup - Thermostat - Thermostat

    Shimadzu LC-10/20 System 4 Using the control module 4.5.2 Method Setup - Thermostat - Thermostat Fig 50: Method Setup - Thermostat - Thermostat Enable Thermostat Enables the temperature control of the thermostat. When unchecked, all other fields on the tab will be grayed and no thermostat control will be performed.

  • Page 73: Method Setup - Thermostat - Time Program

    4 Using the control module Clarity Controls 4.5.3 Method Setup - Thermostat - Time Program Fig 51: Method Setup - Thermostat - Time Program Time Table The initial analysis settings defined on the Thermostat sub- tab can be changed during the analysis by events programmed in the Time Table. Possible events are: Thermostat Off - At specified time, switches of the thermostat.

  • Page 74: Device Monitor

    Shimadzu LC-10/20 System 4 Using the control module 4.5.4 Device Monitor Device Monitor window can be invoked by the Monitor - Device Monitor command from the Instrument window or using the Device Monitor icon. Thermostat Device Monitor serves for monitoring the actual thermostat temperature and allows to switch the thermostat on and off.

  • Page 75: Report Setup

    4 Using the control module Clarity Controls 4.5.5 Report Setup Both parameters set on the Shimadzu LC- 10/20 System Setup - Thermostat tab (valve types used) and parameters set on the Method Setup - Thermostat tab (including the Time Table) can be printed. To do...

  • Page 76: Troubleshooting

    MIMIC libraries (needed by the control module) were probably not installed. Description: Solution:To be able to use the Shimadzu LC-10/20 System control module it is necessary to reinstall the Clarity station. During the installation, the Custom installation must be chosen.

  • Page 77: Vial Plate Numbers

    Shimadzu LC-10/20 System 6 Vial Plate Numbers 6 Vial Plate Numbers The following tables show the Vial Number mapping on the various trays used with Shimadzu LC-10/20 System autosamplers: Tab 6: Vial numbers on vial trays: Rack type Subtype or model...
  • Page 78 6 Vial Plate Numbers Clarity Controls so that each tray has its own number (1-12) and vials on that tray are numbered according to the tray number multiplied by 1000 and the vial position on that tray (based on the tray type): Tab 7: Vial numbers in the Rackchanger: Tray type Vial numbers...

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