Shimadzu LC-40D X3 CL Instruction Manual
  1. Manuals
  2. Brands
  3. Shimadzu Manuals
  4. Laboratory Equipment
  5. LC-40D X3 CL
  6. Instruction manual

Shimadzu LC-40D X3 CL Instruction Manual

Hide thumbs Also See for LC-40D X3 CL:
Table of Contents

Advertisement

Quick Links

228-97197B
Sep. 2022
Solvent Delivery Module for Shimadzu
Liquid Chromatograph
LC-40D X3 CL
LC-40D XR CL
Instruction Manual
Read this manual thoroughly before you use the product.
Keep this manual for future reference.

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the LC-40D X3 CL and is the answer not in the manual?

Questions and answers

Subscribe to Our Youtube Channel

Related Manuals for Shimadzu LC-40D X3 CL

Summary of Contents for Shimadzu LC-40D X3 CL

  • Page 1 228-97197B Sep. 2022 Solvent Delivery Module for Shimadzu Liquid Chromatograph LC-40D X3 CL LC-40D XR CL Instruction Manual Read this manual thoroughly before you use the product. Keep this manual for future reference.
  • Page 2 This page is intentionally left blank.
  • Page 3 Shimadzu representative to request a replacement. • To ensure safe operation, read the accompanying booklet "Safety Guideline" before using the product. • To ensure safe operation, contact your Shimadzu representative if product installation, adjustment, re-installation (after the product is moved), or repair is required.

  • Page 4: Introduction

    • All rights are reserved, including those to reproduce this manual or parts thereof in any form without permission in writing from Shimadzu Corporation. • Nexera and LabSolutions are trademarks of Shimadzu Corporation. Windows is a registered trademark of Microsoft Corporation in the United States and/or other countries.

  • Page 5: Operating Nexera Cl System

    When, for example, analyzing samples that contain complex sample matrices such as serum, plasma or urine, it may be possible to obtain stabilized data by using an appropriate pre-treatment or an internal standard. LC-40D X3 CL LC-40D XR CL...

  • Page 6: Table Of Contents

    Operation in Constant Flow Delivery Mode ....3.2.3 Operation in Constant Pressure Delivery Mode Types of Screens ..........Monitoring Screens ..........Auxiliary Functions Screen (FUNCTION) .......... 3.5.1 Auxiliary Functions (FUNCTION) List ........3.5.2 [PARAMETER] Setting Group LC-40D X3 CL LC-40D XR CL...
  • Page 7 Prior to Inspection and Maintenance ....4.1.2 List of Periodic Inspection and Maintenance ....4.1.3 Check after Inspection and Maintenance Removing the Right Panel Cover and the Filter Holder .............. Removing/Installing the Pump Heads ....LC-40D X3 CL LC-40D XR CL...
  • Page 8 5.1.2 LC-40D XR CL Specifications ......5.1.3 High-Pressure Gradient System ......5.1.4 Low-Pressure Gradient System ......... 5.1.5 Other Specifications Maintenance Parts ..................5.2.1 Consumable Parts ........... 5.2.2 Replacement Parts ..........5.2.3 Maintenance Kit LC-40D X3 CL LC-40D XR CL...
  • Page 9 Contents Index ............LC-40D X3 CL LC-40D XR CL...
  • Page 10 This page is intentionally left blank.

  • Page 11: Overview

    Automatic Plunger Rinsing An automatic rinsing kit is provided as standard for LC-40D X3 CL/LC-40D XR CL . It can rinse the plunger in the back of the plunger seal. When high-salt-concentration buffer solutions are used, in particular, it prevents premature seal failure due to crystallization, extending the service life of plunger seals.

  • Page 12: Component Parts

    SYRINGE,SS-20ESZ 228-66048 NEEDLE ASSY,FOR 228-18216-91 SYRINGE CLAMP,M9120 046-05522-02 DRAIN ADAPTER 228-42204 STANDARD OUT DRAIN 228-42205 CTO OUT DRAIN 228-42206 STRAIGHT JOINT 228-28163 FITTING,L-TYPE 1253 035-61561-12 *1 It contains cautions for use regarding the instrument. LC-40D X3 CL LC-40D XR CL...

  • Page 13: Optional Parts

    1.3 Optional Parts Optional Parts Optional units available for this instrument are listed below. For information about other optional units listed below, contact your Shimadzu representative. n Common Optional Parts for LC-40D X3 CL/LC-40D XR CL Option Part No. Features Switches between up to 4 liquids of low-pressure gradient or mobile phase.
  • Page 14 TFA, use the standard plunger seal. Suction filter with tube and fittings for the low SUCTION FILTER ASSY 228-61905-49 pressure gradient kit or the degassing unit. n Optional Parts for LC-40D X3 CL Option Part No. Features A set of consumable parts and plumbing parts for LC-40D X3 CL.
  • Page 15 For example, if using a Photo Diode Array and an LC/MS, the MR 180 µLⅡmixer is recommended. A larger than recommended mixer can be used to improve mixing performance, but will also increase the gradient delay volume. LC-40D X3 CL LC-40D XR CL...
  • Page 16 The plunger can be rinsed with the automatic rinsing kit at the back part of the plunger seal. LC-40D X3 CL/LC-40D XR CL has the automatic rinsing kit as standard. When using buffer solution, be sure to attach the automatic rinsing kit.

  • Page 17: Parts Identification And Function

    Switches ON/OFF the power. Pressing the switch turns on the power and pressing it again (Power button) turns off the power. The switch is enabled only when the main power switch on the back is on. LC-40D X3 CL LC-40D XR CL...

  • Page 18: Behind

    It detects liquid leaks. If the leak sensor detects a leak, delivery automatically stops, causing an alarm and message on the display. Leak Sensor *1 This is common to the right and left pump heads. LC-40D X3 CL LC-40D XR CL...

  • Page 19: Back

    If the instrument is not used for a long time, turn off the main power switch. Before turning off the main power switch, be sure to turn OFF the power using the power button. "2.5 Name and Functions of the Operation Panel" P.11 LC-40D X3 CL LC-40D XR CL...

  • Page 20: Right Side And Bottom

    Used to install mixer and column holder. To prevent damage during transportation. LC-40D X3 CL has three screws, and LC-40D XR CL has two screws. Shipping Screws (Red) Remove before installation. Leakage Drain Outlet Connect the provided drain tubing. LC-40D X3 CL LC-40D XR CL...

  • Page 21: Name And Functions Of The Operation Panel

    Holding it down again 1 second turns off the power. Button) Press [CE] to cancel the operation. Delivery start/stop, gradient concentration setting, Direct Key timeprogram start and purge can be performed directly. LC-40D X3 CL LC-40D XR CL...
  • Page 22 Note, however, that the following will apply in the case of a system with multiple pumps. - When using CBM-40 CL: The system power can be turned off using the power button of the pump with the lowest optical link number. LC-40D X3 CL LC-40D XR CL...

  • Page 23: Display Area

    Highlighted during pump operation. Highlighted in the low-pressure gradient mode or blinks in the CONC high-speed LC mode. Highlighted during execution of a time program or in the high-speed LC mode. PURGE Highlighted during purging. LC-40D X3 CL LC-40D XR CL...

  • Page 24: Operation Area

    [Enter] Enter Key Confirms input values of the setting items [↑][→] Arrow Keys Moves the cursor or switches the screen [↓][←] [·] to [9] Numeric Keys Inputs values for the setting items. LC-40D X3 CL LC-40D XR CL...
  • Page 25 Description Acceptable part of [↑] key input. Acceptable part of [→] key input. Acceptable part of [↓] key input. Acceptable part of [←] key input. LC-40D X3 CL LC-40D XR CL...

  • Page 26: Settings For The Basic Operation

    Before operating this instrument, it is necessary to set the flow rate and the pressure limit to protect flow line parts such as columns. See the product specifications before use. "5.1.1 LC-40D X3 CL Specifications" P.125 "5.1.2 LC-40D XR CL Specifications" P.126 3.1.1 Prior to Key Operation Touching the central part of the operation panel displays the operation keys that the user can operate.

  • Page 27: Setting Maximum Pressure Limit

    The following is an example to set the maximum pressure [P.MAX] to 15.0 MPa. Press [→] three times on the initial screen. The PARAMETER setting group is displayed. Press [Enter]. The cursor blinks at the input position indicating that [P.MAX] can be input. LC-40D X3 CL LC-40D XR CL...

  • Page 28: Setting Minimum Pressure Limit

    Press [→] three times on the initial screen. The PARAMETER setting group is displayed. Press [↓] once and on the [P.MIN] screen, press [Enter]. The cursor blinks at the input position indicating that [P.MIN] can be input. LC-40D X3 CL LC-40D XR CL...

  • Page 29: Operation

    If [P.MIN] is set to "0", solvent delivery will not stop and an alarm will not sound even if the pressure has dropped. The minimum pressure can be set to 130 MPa max. for LC-40D X3 CL and 60 MPa max. for LC-40D XR CL. "Setting the minimum pressure during delivery《P.MIN》" P.31 Operation 3.2.1 Preparation before Operation...

  • Page 30: Operation In Constant Flow Delivery Mode

    To set 1 mL/min, press [1] and [Enter]. Press [PUMP], and then [ENTER]. [PUMP] on the display is highlighted and the pump starts operation. Check that the pump outlet pressure is increasing on the pressure indication display. LC-40D X3 CL LC-40D XR CL...

  • Page 31: Operation In Constant Pressure Delivery Mode

    "Switching the delivery mode《MODE CHANGE》(Only for LC-40D XR CL)" P.38 After changing the mode, press [CE] once. The initial screen of the constant pressure delivery mode is displayed. LC-40D X3 CL LC-40D XR CL...
  • Page 32 If increasing the flow rate does not increase the pressure, stop increasing the flow rate at 1 mL/min. To stop operation, press [PUMP], and then [ENTER]. [PUMP] on the display stops being highlighted and the pump stops operation. LC-40D X3 CL LC-40D XR CL...

  • Page 33: Types Of Screens

    [FUNCTION] or VP functions screen [VP]. Move the cursor to the desired option with [↓] or [↑] and press [→] to display the screen. Press [←] to return to the previous screen. And Press [CE] to return to the initial screen. LC-40D X3 CL LC-40D XR CL...

  • Page 34: Monitoring Screens

    ID/SV》 When the system controller is connected, one of the pump connection addresses (A, B, C, or D) of the system controller is displayed on the lower left side of the screen. LC-40D X3 CL LC-40D XR CL...
  • Page 35 Constant flow pumping Pumping Methods "Switching the delivery mode《MODE CHANGE》(Only for LC-40D XR CL)" P.38 0.1 to 2 mL/min Flow Rate * Pumps should satisfy the above flow rate range in the high -pressure gradient mode. LC-40D X3 CL LC-40D XR CL...
  • Page 36 3 Operation Item Specification LC-40D X3 CL: 5 to 130 MPa Delivery Pressure LC-40D XR CL: 5 to 70 MPa Isocratic, two-solvent high-pressure gradient, two-solvent Pumping Modes low-pressure gradient The Auto-Diagnostics function may not work in case of a significant pressure decrease, such as when a large bubble enters the pump head.
  • Page 37 [Connected] is displayed when the instrument is connected with a PC/WS. This screen is displayed only when the CBM-40 CL is connected. [Disconnected] is displayed when the instrument is not connected with a PC/WS. LC-40D X3 CL LC-40D XR CL...

  • Page 38: Auxiliary Functions Screen (Function)

    Initial value: 0 Sets the minimum pressure limit Set value: P.MIN P.31 for solvent delivery. (LC-40D X3 CL) 0 to 130.0 MPa (LC-40D XR CL) 0 to 60.0 MPa Makes fine adjustment of Initial value: 0.45 COMP compressibility correction of P.32...
  • Page 39 (SYS) system used. 4: Low-pressure gradient mode Reduces the flow rate without SYSTEM stopping the pumping when the Initial value: 0 P.40 PROTECT pressure exceeds the P.MAX Set value: 0: Clear 1: Set value. LC-40D X3 CL LC-40D XR CL...
  • Page 40 [FILE] operation group This function is not used because events are not used in the CL model. Name Description Remark Initial value: 0 FILE NUMBER Selects a program file No. Set value: 0 to 9 LC-40D X3 CL LC-40D XR CL...

  • Page 41: Parameter] Setting Group

    0 to 60.0 MPa 0.1 MPa 0 to 1326 kgf/cm 0 to 612 kgf/cm 1 kgf/cm 0 to 1300 bar 0 to 600 bar 1 bar 0 to 19000 psi 0 to 8706 psi 1 psi LC-40D X3 CL LC-40D XR CL...
  • Page 42 In other cases or when rigorous adjustment is required Connect a resistance tube (or column) that can create pressure similar to the desired delivery pressure at 1.0 mL/min or less and wait until delivery becomes stable. LC-40D X3 CL LC-40D XR CL...
  • Page 43 Mobile Phase Port Solvent A Solvent B Low-Pressure Gradient Kit Solvent C Solvent D Solvent A Reservoir Switching Valve Solvent B The value cannot be input if the instrument has no internal solenoid valve. LC-40D X3 CL LC-40D XR CL...
  • Page 44 Do not change the factory default value. Set "ON" (close) / "OFF" (open) of the [EVENT2] output (relay contact) on the back of the instrument. EVENT2 Output Value Relay 2 OFF Relay 2 ON LC-40D X3 CL LC-40D XR CL...
  • Page 45 The value can be set in the range from 0.1 mL/min to 10.0 mL/min in 0.1 mL/min increments. The flow rate set here is approximate for replacing the mobile phase in the pump. LC-40D X3 CL LC-40D XR CL...
  • Page 46 • The approximate delivery amount necessary for replacing the rinse solution in the rinsing flow path is approximately 10 mL. • To replace the rinse solution in the rinsing flow path, select "4" and operate the pump approximately 3 minutes. LC-40D X3 CL LC-40D XR CL...

  • Page 47: Control] Setting Group

    The plunger stops at the middle position. n Zero adjustment of pressure sensor《ZERO ADJUST》 Set the reference value for the zero point of the pressure sensor. Open the drain valve to remove pressure completely. Press [Enter]. LC-40D X3 CL LC-40D XR CL...

  • Page 48: System] Setting Group

    The instrument is operated independently (local mode). n Setting the link address《LINK ADDRESS》 Set the address (channel No.) used to connect with the system controller. Address Function 1 to 12 A channel number for connecting with the system controller LC-40D X3 CL LC-40D XR CL...
  • Page 49 If the SELECT EVENT1 function is used, the corresponding EVENT parameter is disabled. n Setting the function of the EVENT2 output terminal《SELECT EVENT2》 Not used in CL model. Do not change the factory default value. LC-40D X3 CL LC-40D XR CL...
  • Page 50 [P.MAX]. Set Value Function Do not use system protection. Use system protection. To clear a [P.MAX] error, press [CE]. The alarm stops and the flow rate returns to that before the error. LC-40D X3 CL LC-40D XR CL...
  • Page 51 Input the serial number of the flow path switching valve connected to the SV connector on the back of the instrument. n Selecting the flow path of the solenoid valve unit《EXT.SV CHANNEL》 Not used in CL model. LC-40D X3 CL LC-40D XR CL...
  • Page 52 Not used in CL model. n Setting the unit of the displayed pressure《PRESSURE UNIT》 Set the unit of the displayed pressure. Unit of Displayed Value Value kgf/cm 1 kgf/cm = 0.098 MPa = 0.98 bar = 14.2 psi LC-40D X3 CL LC-40D XR CL...
  • Page 53 　　→Set value: 355（from the table above） (The capacity of the mixer will be automatically recognised by the column oven) 3) Use the mixer instllation kit (without the mixer recognition device) 　　→Set value: 40 (0 µL （from the table above） + 40 µL (mixer capacity)) LC-40D X3 CL LC-40D XR CL...

  • Page 54: Utility] Setting Group

    Sets the time elapsed before the operation panel automatically turns to the sleep mode, when no operations are performed on the instrument. The setting range is from 0 to 10 (min), in which the step can be set by minute. LC-40D X3 CL LC-40D XR CL...
  • Page 55 When the optional low-pressure gradient kit or reservoir switching valve is installed inside, pressing [PURGE] when starting purging does not display the confirmation screen. The confirmation screen, however, is displayed when purging is stopped. LC-40D X3 CL LC-40D XR CL...

  • Page 56: File] Operation Group

    [FILE NUMBER] above) is copied to the file of a specified number. Input a file number with the numeric keypad and press [Enter]. n Deleting a file《FILE DELETE》 The time program of the currently selected file is deleted. Press [Enter]. LC-40D X3 CL LC-40D XR CL...

  • Page 57: Vp Functions Screen

    DGU OP TIME P.52 connected to the instrument. *1 Normally one solvent can be set for the mobile phase level monitoring function. Only in the low-pressure gradient mode, four solvents (A to D) can be set. LC-40D X3 CL LC-40D XR CL...
  • Page 58 Turns on/off Auto-Diagnostics. P.68 1 In the calibration support group, an invalid password prevents the user from going further than [OPERATION MODE] even if the user presses [Enter]. 2 Do not change the factory default value. LC-40D X3 CL LC-40D XR CL...

  • Page 59: Product Information Group (Product Info)

    Displaying the delivery amount of the right plunger seal《R SEAL USED》 The screen displays the total delivery amount of the currently used plunger seal and the delivery amount that requires replacement (unit: L). LC-40D X3 CL LC-40D XR CL...
  • Page 60 Note that delivery continues. Input the set value with the numeric keypad and press [Enter]. Setting the value to 0 % disables the low mobile phase level alarm. LC-40D X3 CL LC-40D XR CL...
  • Page 61 After press [→], press [↓] several times to display the operation log records in order. The figure shows an example that "the password was changed on April 1, 2019". LC-40D X3 CL LC-40D XR CL...
  • Page 62 LC-Workstation. • Approximate time to replace the vacuum pump of the degassing unit is 3 years or 8000 hours of operation time. Contact your Shimadzu representative if you need to replace the vacuum pump. LC-40D X3 CL LC-40D XR CL...

  • Page 63: Validation Support Information Group (Validation)

    "00:00:00" when the power is turned off. The value is transmitted when the system controller is connected for control. To delete the input value, press [del]. The figure is an example of 3:01:01 p.m. LC-40D X3 CL LC-40D XR CL...
  • Page 64 After wetting and testing the leak sensor, wipe away the water on the tray completely. When wiping away the water, do not apply pressure to the leak sensor, the sensitivity decreases if the sensor unit touches the wall. "4.14 Cleaning of the Leak Tray" P.122 LC-40D X3 CL LC-40D XR CL...
  • Page 65 Before measurement, make sure that the compressibility correction parameter of the solvent is correctly set. "Setting the compressibility of solvent《COMP》" P.32 LC-40D X3 CL LC-40D XR CL...
  • Page 66 5 mL in a measuring flask. In the [FLOW CHECK] screen, press [Enter]. In the measurement method selection screen, input "0". Input the measured flow rate. The value can be set between 0.1 mL/min and 3.0 mL/min. LC-40D X3 CL LC-40D XR CL...
  • Page 67 Measure the weight (g) before delivery. In the [FLOW CHECK] screen, press [Enter]. In the measurement method selection screen, input "1". Input the measured flow rate. The value can be set between 0.1 mL/min and 3.0 mL/min. LC-40D X3 CL LC-40D XR CL...
  • Page 68 To use a flow meter Set the flow rate and start delivery. After confirming the delivery stabilizes, measure the flow rate. In the [FLOW CHECK] screen, press [Enter]. In the measurement method selection screen, input "2". LC-40D X3 CL LC-40D XR CL...
  • Page 69 For example, if the set flow rate is 1 mL/min and the criterion is ±2 %, input 0.02 mL. Input the flow rate measured with a flow meter. The flow rate accuracy (%) and pass/fail result are displayed. LC-40D X3 CL LC-40D XR CL...
  • Page 70 (P.MIN) are automatically set. Then the flow rate is automatically set to 1.5 mL/min and 0.5 mL/min, and whether the P.MAX and P.MIN functions work correctly is checked. The result is displayed after checking is completed. LC-40D X3 CL LC-40D XR CL...

  • Page 71: Calibration Support Information Group (Calibration)

    If the password does not match, the user cannot go further. n Setting the operation mode《OPERATION MODE》 This function is not used in the CL model. Do not change the factory default value. The factory default value : 0 LC-40D X3 CL LC-40D XR CL...
  • Page 72 If [1] (On) is set, a password input screen is displayed when the user enters the setting screen. If the user modifies the setting or inputs the password to enter the setting screen, the operation is recorded in the operation log. "Displaying the operation log《OPERATION LOG》" P.51 LC-40D X3 CL LC-40D XR CL...
  • Page 73 If the password input is not correct, the message "PASSWORD WRONG" is displayed. At this stage, the password has not been changed yet. Press [Enter] to return to the title screen. LC-40D X3 CL LC-40D XR CL...
  • Page 74 Displays the serial number of the system controller which controls the instrument. Showing the S/W version number《S/W ID》 Displays the S/W name (the same name as the model name) and version of CBM which controls the instrument. LC-40D X3 CL LC-40D XR CL...
  • Page 75 Use Default Gateway. Automatic acquisition of IP address from DHCP server. Setting IP address《IP ADDRESS》 Sets IP address of the controller which controls the instrument. Enter the value using the numeric keypad and press [Enter]. LC-40D X3 CL LC-40D XR CL...
  • Page 76 Dip the sensor unit at the bottom of the leak sensor into water. Upon detection of water, the leak sensor value (Actual Level) in the screen increases. Read the maximum value of the leak sensor value (Actual Level). Example) The maximum value here is "160". LC-40D X3 CL LC-40D XR CL...
  • Page 77 Setting the pressure sensor sensitivity correction factor《PRESS COMP FACTOR》 Not used in CL model. Do not change the factory default value. This allows service personnel to set the pressure sensor sensitivity correction factor. LC-40D X3 CL LC-40D XR CL...
  • Page 78 [OPERATION MODE] is set to "1" (compatible mode) and the instrument is controlled with the system controller. n Turning on/off Auto-Diagnostics《AUTO DIAGNOSTICS》 This allows the user to turn on/off Auto-Diagnostics. Set Value Setting Method Auto-Diagnostics is off. LC-40D X3 CL LC-40D XR CL...

  • Page 79: Creating Time Program

    CCONC 0 to100 % Only active when [PUMP 0.1 % CONFIG(SYS)] = 4 Specifies concentration of mobile phase D Minimum unit: DCONC 0 to100 % Only active when [PUMP 0.1 % CONFIG(SYS)] = 4 LC-40D X3 CL LC-40D XR CL...

  • Page 80: Time Program Setting Screen

    3.7.2 Time Program Setting Screen To create a time program, access the edit screen as described below. Press [CE]. The initial screen is displayed. Press [→] twice. The auxiliary functions screen [FUNCTION] is displayed. LC-40D X3 CL LC-40D XR CL...

  • Page 81: Creating A Time Program

    (initial flow rate: 1 mL/min). FUNC Command VALUE(mL/min) Step TIME (minute) Setting Option Input a Command Value 15.00 FLOW 2.0000 20.00 FLOW 5.0000 30.00 STOP LC-40D X3 CL LC-40D XR CL...
  • Page 82 Press [Enter]. The [FLOW] command is selected, which enables the user to input the flow rate. Press [5] and [Enter] to set the flow rate. To set step 3 Press [3], [0], and [Enter]. LC-40D X3 CL LC-40D XR CL...

  • Page 83: Creating A Low-Pressure Gradient Program

    0 % to 100 % with the minimum unit of 0.1 %. n Example of time program setting Displays the time program setting screen. "3.7 Creating Time Program" P.69 Press [Enter] and input the time with the numeric keypad. LC-40D X3 CL LC-40D XR CL...

  • Page 84: Deleting A Step

    3.7.6 Starting and Stopping a Time Program n Starting a Time Program To start a time program after setting it, perform the following. Press [RUN] to start the program. [RUN] on the display is highlighted. LC-40D X3 CL LC-40D XR CL...

  • Page 85: Commands Used For Time Programs Only

    [↓] several times until [STOP] is displayed. Press [Enter] to set a [STOP] command. When the time program is executed continuously, do not set a [STOP] command at the end of the program. LC-40D X3 CL LC-40D XR CL...

  • Page 86: Delivery In The Low-Pressure Gradient Mode

    Solvent A concentration (%) = 100 (%) - Solvent B concentration (%) - Solvent C concentration (%) - Solvent D concentration (%) For example, when setting the solvent A to D concentrations to 25%, input values as shown in the figure. LC-40D X3 CL LC-40D XR CL...
  • Page 87 Solvent is purged at the concentration set on the concentration setting screen. Solvent A is purged. Solvent B is purged. Solvent C is purged. Solvent D is purged. Close the drain valve and start delivery. LC-40D X3 CL LC-40D XR CL...

  • Page 88: Connection With The System Controller Or Workstation

    For details, refer to the system guide. 3.10 Connection to External Input/Output Terminals In the CL model, do not connect another external device with the black-white event cable. Wiring is done by service personnel during installation. LC-40D X3 CL LC-40D XR CL...

  • Page 89: Periodic Inspection And Maintenance

    Periodic Inspection and Maintenance It is necessary to perform periodic inspections of this instrument to ensure its safe use. It is possible to have these periodic inspections performed by Shimadzu service personnel on a contractual basis. For information regarding the maintenance inspection contract, contact your Shimadzu representative.
  • Page 90 "4.4 Replacing the Plunger Seal" P.92 "4.7 Performing an Ultrasonic Cleaning of the Check Valve" P.110 Ultrasonic bath Used for cleaning parts. "Gradient Mixer for High-Pressure Gradient Analysis" "4.16 Cleaning of the Automatic Rinsing Kit" P.123 LC-40D X3 CL LC-40D XR CL...
  • Page 91 4.1 Periodic Inspection and Maintenance Item Description Inspection/Maintenance Item Used for gripping the "4.4 Replacing the Plunger Tweezers plunger seal and backup Seal" P.92 ring. LC-40D X3 CL LC-40D XR CL...

  • Page 92: List Of Periodic Inspection And Maintenance

    Replacement of Suction Replace when particulates in mobile 2 years P.117 Filter phase clog the filter. Replacement of Drain Replace when there is a fluid leak from 3 years P.119 Valve the drain valve. LC-40D X3 CL LC-40D XR CL...

  • Page 93: Check After Inspection And Maintenance

    • Return the right panel cover, filter holder and tubes after completing the work. When the parts management tool (PARTS MGMT TOOL) is not used, input the part number when replacing a part not registered in the PARTS MGMT TOOL. "Inputting a replacement part number《PART REPLACEMENT》" P.51 LC-40D X3 CL LC-40D XR CL...

  • Page 94: Removing The Right Panel Cover And The Filter Holder

    While lightly pressing the cylinder-shaped projection (a) inside the right panel cover, slide the cover to the front. While pressing the knob at the top of the filter holder (A), remove it toward the front. LC-40D X3 CL LC-40D XR CL...

  • Page 95: Removing/Installing The Pump Heads

    Press [CE] to display the initial screen. Press [→] twice. Press [↓] once, move the cursor to [CONTROL], and press [→]. [PLUNGER SET] menu is displayed. LC-40D X3 CL LC-40D XR CL...
  • Page 96 (C) of the SUS pipe R with an 8-mm wrench. Hold the check valve OUT side male nut ("a" in the figure) of the SUS pipe R and then slightly lift and pull out the SUS pipe R. LC-40D X3 CL LC-40D XR CL...
  • Page 97 (C) of the SUS pipe R with an 8-mm wrench. Hold the check valve OUT side male nut ("a" in the figure) of the SUS pipe R and then slightly lift the SUS pipe R. LC-40D X3 CL LC-40D XR CL...
  • Page 98 (upward U-shape), and use the syringe (D) to suction out the rinse fluid from the rinse flow line. Place the removed cap (B) and rinse solution bottle on the reservoir tray on the top of the instrument. LC-40D X3 CL LC-40D XR CL...
  • Page 99 The plunger may be damaged if the pump head is forcefully inserted. Tighten the left and right hexagonal socket screws 90 degrees at a time, and then firmly tighten using the long side of the Allen wrench. LC-40D X3 CL LC-40D XR CL...
  • Page 100 Hold the check valve side end of the SUS pipe R ("a" in the figure) and then insert the male nut into the check valve OUT (A). LC-40D X3 CL LC-40D XR CL...
  • Page 101 Attach the syringe needle (C) to the end of the FEP tubing (E) (upward U-shape), and use the syringe (D) to suction out the rinse solution from the rinse flow line. Return the rinsing kit to the reservoir tray. LC-40D X3 CL LC-40D XR CL...

  • Page 102: Replacing The Plunger Seal

    Prior to the maintenance Perform the preparatory work. "4.1.1 Prior to Inspection and Maintenance" P.79 4.4.1 Replacing the Plunger Seal and Backup Ring Remove the pump head, SUS pipe, and inlet tube by following the instructions "Removing the pump head" P.85. LC-40D X3 CL LC-40D XR CL...
  • Page 103 With the metal part (a) of the seal installer/remover (A) facing downward, insert the seal installer/remover (A) vertically into the backup ring (B). Turn the seal installer/remover (A) 180 degrees to 360 degrees clockwise. LC-40D X3 CL LC-40D XR CL...
  • Page 104 Turn the seal installer/remover (A) clockwise until it comes into contact with the pump head (C). LC-40D X3 CL LC-40D XR CL...
  • Page 105 2-propanol. Remove the cap (c) from the seal installer/remover (A). Wipe off the white protrusion (d) and the surrounding area of the seal installer/remover (A) with a wiping paper soaked in 2-propanol. LC-40D X3 CL LC-40D XR CL...
  • Page 106 Take the new plunger seal (D) out of the beaker and put it on the white protrusion (d) of the seal installer/remover (A). Be sure that the plunger seal is moistened with 2-propanol when putting it on the seal installer/remover. LC-40D X3 CL LC-40D XR CL...
  • Page 107 Insert the seal installer/remover vertically all the way into the plunger seal and slowly pull out the seal installer/remover upward. The new backup ring (B) will be installed into the pump head (C). LC-40D X3 CL LC-40D XR CL...
  • Page 108 4 Maintenance Put the cap (c) on the white protrusion (d) of the seal installer/remover. Attach the pump head, SUS pipe, and inlet tube by following the instructions in "Installing the pump head" P.89. LC-40D X3 CL LC-40D XR CL...

  • Page 109: Resetting The Delivery Amount

    If the above occurs even after replacing the plunger seal, it is possible that there are scratches on the surface of the plunger. If there are scratches on the plunger, replace it with a new plunger. "4.5 Cleaning/ Replacing the Plunger and Diaphragm" P.100 LC-40D X3 CL LC-40D XR CL...

  • Page 110: Cleaning/ Replacing The Plunger And Diaphragm

    Part Type Part No. Remark Supplied with a Plunger Holder Assy Consumable part 228-52069-44 diaphragm n Prior to the inspection and the maintenance Perform the preparatory work. "4.1.1 Prior to Inspection and Maintenance" P.79 LC-40D X3 CL LC-40D XR CL...

  • Page 111: Removing The Pump Head And The Head Holder

    (A) using an Allen wrench (M4) by alternating between the left and right screw. Remove the head holder. Gently pull the head holder horizontally along the plunger. If the head holder is not removed carefully, the plunger may become damaged. LC-40D X3 CL LC-40D XR CL...
  • Page 112 Allen wrench (M4) (D). The head holder (A) can be loosened by pushing the pump body (C) using the 2 hexagonal socket screws (B). LC-40D X3 CL LC-40D XR CL...

  • Page 113: Cleaning And Replacement Of The Plunger And The Diaphragm

    Do NOT allow foreign particles or crystals into the head holder. Foreign particles from the mobile phase solution or crystals from the buffer solution getting into the head holder may cause scratches on the surface of the plunger. LC-40D X3 CL LC-40D XR CL...
  • Page 114 • Be careful not to get any grease on the diaphragm or the body. • Attach the diaphragm so that it will not become detached from the plunger holder. If the diaphragm becomes detached, wipe away any grease and perform from Procedure 4 again. LC-40D X3 CL LC-40D XR CL...
  • Page 115 (To replace the left plunger, press [2] and [Enter].) [PUMP] on the display is highlighted and after a while stops being highlighted. The right plunger is now at the frontmost position. "Setting the plunger stop position《PLUNGER SET》" P.37 LC-40D X3 CL LC-40D XR CL...
  • Page 116 Replacing the Plunger Seal" P.92. Attach the pump head, SUS pipe, and inlet tube and set the seal rinsing kit back in place by following the instructions in "Removing the pump head" P.85. LC-40D X3 CL LC-40D XR CL...

  • Page 117: Rinsing The Check Valve

    2-propanol, crystals will be precipitated, which can cause damage to the plunger seal or cause problems with the check valve. System Guide "Precautions When Replacing the Mobile Phase" Replace the solvent in the reservoir bottle with 2-propanol. LC-40D X3 CL LC-40D XR CL...
  • Page 118 C) of the resistor tube inlet and outlet to the coupling (D) with an 8 mm wrench and tighten it with the 8 mm wrench. Check the pumping stability after cleaning the check valves. "Checking the pulse《PULSE CHECK》" P.55 LC-40D X3 CL LC-40D XR CL...
  • Page 119 4.6 Rinsing the Check Valve Replace the solvent in reservoir bottle with the original mobile phase. System Guide "Precautions When Replacing the Mobile Phase" LC-40D X3 CL LC-40D XR CL...

  • Page 120: Performing An Ultrasonic Cleaning Of The Check Valve

    (B). Never disassemble the check valve. Performance cannot be guaranteed if disassembled. (For LC-40D X3 CL) Do NOT turn the inlet check valve (A) upside down. If you do, the internal parts will fall out. LC-40D X3 CL LC-40D XR CL...
  • Page 121 Hand tightening and 60 to 90° with wrench • LC-40D XR CL: Hand tightening and 90° with wrench Attach the SUS pipe and inlet tube by following the instructions in "Installing the pump head" P.89. LC-40D X3 CL LC-40D XR CL...

  • Page 122: Replacing The Check Valve

    P.89. Turn the drain valve counterclockwise to open it. Press [Enter] once. The cursor blinks at the [FLOW] input position. Press [2], [. ], [0], and [Enter]. [FLOW] is set to "2.0000" and confirmed. LC-40D X3 CL LC-40D XR CL...
  • Page 123 Press [PUMP] and confirm the solvent continues to flow out from the drain valve. [PUMP] on the display is highlighted and the solvent flows out of the drain tube. There is no problem with the valve if the solvent continues to flow out. LC-40D X3 CL LC-40D XR CL...

  • Page 124: Inspecting The Line Filter

    (Pumping pressure: 1 MPa to 5 MPa) For example, attach a stainless tube with ID0.3 x 2000mm* *1: Stainless tubing ID0.3 x 1000mm (Part No.: 228-53184-96) 2pc and Coupling (Part No.: 228-16004-13) 1pc can be substituted. LC-40D X3 CL LC-40D XR CL...

  • Page 125: Replacing The Line Filter

    Wipe with a cotton swab, etc., to wipe away any dirt from the line filter port (a). Attach the new line filter (A) to the line filter port (a) by hand, and tighten it 45° using a 10 mm wrench. LC-40D X3 CL LC-40D XR CL...
  • Page 126 50% methanol solution at a pressure of approx.10 MPa for approx. 30 min. (For example, attach a stainless steel tube with ID 0.1 x 2000 mm (Part No.: 228-57908-41) and adjust the flow rate.) LC-40D X3 CL LC-40D XR CL...

  • Page 127: Cleaning/Replacing The Suction Filter

    System Guide "Precautions When Replacing the Mobile Phase" Pull the suction filter (A) from the suction tubing (B). Place the suction filter into a clean container with 2-propanol, and clean it for 5 minutes using an ultrasonic bath. LC-40D X3 CL LC-40D XR CL...
  • Page 128 Check for air bubbles inside the suction tubing. Replace the suction filter if air bubbles are present. If Ghost peaking occurs, rinse the suction filter. Refer to System Guide "5.3.2 Symptoms Presumably Due to an Error, Based on a Chromatogram". LC-40D X3 CL LC-40D XR CL...

  • Page 129: Replacing The Drain Valve

    If the drain valve ASSY is forcefully inserted or is not inserted straight, the seal portion may become deformed, resulting in leakage. Tighten the drain valve ASSY clockwise. LC-40D X3 CL LC-40D XR CL...

  • Page 130: Replacing The Air Filter (Right Panel)

    Air Filter Element, Non Woven, for Right Consumable part 228-53924-06 Panel Open the right panel. Remove the right panel cover. While lightly pressing the cylinder-shaped projection (a) inside the right panel cover, slide the cover to the front. LC-40D X3 CL LC-40D XR CL...
  • Page 131 4.13 Replacing the Air Filter (Right Panel) While pressing the knob at the top of the filter holder (A), remove it toward the front. Slide the air filter (B) to attach/remove Attach the filter holder and the right panel. LC-40D X3 CL LC-40D XR CL...

  • Page 132: Cleaning Of The Leak Tray

    Wipe off detergent with a cloth soaked in water and wrung tightly and then wipe off water with a dry cloth. Do NOT leave any part wet nor use thinner for cleaning. Doing so may cause rust or discoloration. LC-40D X3 CL LC-40D XR CL...

  • Page 133: Decontamination Cleaning

    5 minutes using an ultrasonic bath. Pour new 10% 2-propanol water into the rinse solution bottle, and return the cap and rinse solution bottle back to their original positions. LC-40D X3 CL LC-40D XR CL...
  • Page 134 4 Maintenance Replace the rinse tube if the inside is dirty. LC-40D X3 CL LC-40D XR CL...

  • Page 135: Technical Information

    *1 When water is pumped at 10 to 40 MPa, with water and room temperature constant between 20 to 30 °C. *2 When water is pumped at 40 to 60 MPa, with water and room temperature constant between 20 to 30 °C. LC-40D X3 CL LC-40D XR CL...

  • Page 136: Lc-40D Xr Cl Specifications

    Larger value of either ±2 % or ±1.0 MPa Suction Filter 10 µm Line Filter 5 µm Commands for flow rate, pressure, [EVENT], Time Program [LOOP] (for program repetition), 320 steps (total of 10 program files) LC-40D X3 CL LC-40D XR CL...

  • Page 137: High-Pressure Gradient System

    Range of Set 0 % to 100 % (in steps of 0.1 %) Concentrations ±0.5 %(1 mL/min, 10 MPa) Concentration Accuracy (for binary gradient with water/caffeine solution) Flow Rate Possible 0.0001 mL/min to 10 mL/min LC-40D X3 CL LC-40D XR CL...

  • Page 138: Other Specifications

    Altitude 2000 m max. Install indoors. Plunger Rinsing Line Automatic rinsing kit as standard equipment Pressure Limiter Operations Upper/lower limits can be set Exist (Error display and stop at the time of Error Display malfunction) LC-40D X3 CL LC-40D XR CL...
  • Page 139 0 kPa to 50 kPa Pressure Materials for Parts PTFE, PEEK, fluororubber Exposed to Liquid 4 °C to 35 °C Operating Temperature Available Flow Rate 0.0001 mL/min to 10 mL/min Available pH 1 to 14 LC-40D X3 CL LC-40D XR CL...

  • Page 140: Maintenance Parts

    5 Technical Information Maintenance Parts LC-40D X3 CL LC-40D XR CL...

  • Page 141: Consumable Parts

    LC-40D XR CL Inlet Check Valve ASSY, 2 pieces 228-48249-97 LC-40D XR CL 228-53334-96 LC-40D X3 CL Outlet Check Valve ASSY 228-45705-43 LC-40D XR CL DIAPHRAGM 2PCS 228-55272-41 AIR FILTER FOR RIGHT PANEL 228-53924-06 Filter only LC-40D X3 CL LC-40D XR CL...

  • Page 142: Replacement Parts

    228-70044 DRAIN TUBE ASSY 228-25495-93 For use between the inlet block ETFE TUBE 1.6(O.D.)X0.8(I.D.) 228-18495-01 and the check valve For plumbing between the inlet MALE NUT 1.6 PEEK 228-35403 block and the check valve LC-40D X3 CL LC-40D XR CL...

  • Page 143: Maintenance Kit

    5.2 Maintenance Parts 5.2.3 Maintenance Kit A set of consumable parts is provided as Maintenance Kit n Maintenance Kit for LC-40D X3 CL (Parts No. 228-53265-45) Part Name Part No. Quantity Remarks PLUNGER SEAL,BACKUP RING UHP 228-52711-93 With backup ring 　...
  • Page 144 Validation Support Information Group (VALIDATION) Initializing VP Functions Screen LC-40D XR CL Specifications Leak Sensor Line Filter 114, 115 Liquid-Contacting Part Materials Low-Pressure Gradient Low-Pressure Gradient System Main Power Switch Maintenance Information Group (MAINTENANCE) Maintenance Kit LC-40D X3 CL LC-40D XR CL...

This manual is also suitable for:

Lc-40d xr cl

Table of Contents

Save PDF