Shimadzu Shim-pack GIST Series Instruction Manual

 Introduction
To maintain and maximize peak performance of Shim-pack
GIST/GISS series columns, and to ensure the long life and stability
of columns, please read the following instructions before use.
 Specifications
The product specifications of Shim-pack GIST/GISS series
columns are as follows.
Type Columns
Shim-pack GIST C18
Shim-pack GIST C18-AQ
Shim-pack GISS C18
Reversed-
Shim-pack GIST C8
Phase
Shim-pack GIST Phenyl
Shim-pack GIST Phenyl-
Hexyl
Normal-
Shim-pack GIST NH2
Phase
 Operating Precautions
Check if anything is missing or damaged. If there are any signs of
damage, notify your local Shimadzu representative at once.
Each of the Shim-pack GIST/GISS series columns is delivered with
a Column Performance Report. The information supplied in the
report include the lot number, column serial number, and
chromatographic test conditions. Please keep the report for future
reference.
 Column Performance
Shim-pack GIST/GISS series packing materials are subjected to a
rigorous array of QC tests, with special emphasis on reagent purity,
raw material traceability, consistency in raw materials, and quality
of final products. A detailed analysis of all of the physical and
chemical properties of these columns, combined with tests for
chromatographic selectivity and column packing material efficiency,
ensure that each lot of these columns are identical to all previous
lots and column-to-column reproducibility is of the highest order.
Shim-pack GIST/GISS series columns are shipped with the solvent
used for the final QC test of the column, as detailed in the Column
Performance Report delivered with the column.
In the case of NH2 columns, when switching between solvents with
significantly different polarities, first purge the column with a
mutually miscible solvent such as Isopropyl alcohol at a reduced
flow rate (approximately 50% lower than the normal flow rate).
Flush the column with the solvent five times more than the column
volume (e.g. 10 mL for a 150 mm x 4.6 mm I.D. column).
High Performance Packed Column for HPLC
Shim-pack
INSTRUCTION MANUAL
Base
Stationary Phase
Material
Octadecyl Groups
(C18)
High purity
Octyl Groups (C8)
porous
Phenyl Groups
spherical
Phenyl-Hexyl
silica
Groups
Aminopropyl Groups
GIST/GISS
 Column Installation
The flow direction of the column is shown on the column (→).
When installing the column, ensure that the flow direction matches
the mobile phase flow direction.
Use PEEK tubing (UHPLC: SUS tubing) with an inner diameter of
0.25 - 0.3 mm (UHPLC: 0.1 - 0.2 mm) and an outer diameter of 1.6
mm.
Use the shortest possible tubing connection from the injector to the
column to minimize peak broadening.
The column should be connected with male nuts. Ensure that the
fittings are connected properly to avoid creating dead volume
between the tubing and the column interface. Male nuts can be
ordered by referring to the part number below.
Item name
Male nut, PEEK
Male nut 1.6 MN
Ferrule 1.6 F
UHPLC Fitting 2 S
NOTE Stains or air in the flow line may deteriorate the column. Before
connecting the column, be sure to flow the mobile phase to flush
the flow line.
If peaks are tailing more on the early eluting compounds than later
eluting compounds, there is a possibility that there is a dead volume.
In such case, check that all column connections are properly
connected.
Also, make sure to use appropriate internal diameter and length size
of tubing at the injector and detector, especially when using semi-
micro size columns, to avoid system dead volumes.
 Sample
Samples should be dissolved in an eluent or solvent weaker than the
mobile phase, which helps avoid sample precipitation at column
inlet/head and inconsistent retention values.
 Clogging of column
The most common cause of the increase of column back pressure or
split peaks is blockage of the inlet filter by sample particulates,
particles created by aging pump seals, or large quantities of
lipophilic compounds adsorbing to the head of the column.
• Filtrate the mobile phase using a 0.45 μm membrane filter before
using the column.
• Installing "Ghost Trap DS" between the pump and injector can
efficiently remove particulates or contaminants in the mobile
phase.
ERAD-0001-9007
Series
P/N Remarks
228-18565-84 5 pcs
228-16001 1 pc
228-16000-10 1 pc
228-56867-41 1 pc
Pressure
20 MPa
130 MPa
130 MPa
130 MPa