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INTRODUCTION
Phase contrast microscopy provides a means to observe transparent specimens, which are very difficult to observe
under bright field illumination. Another advantage of phase microscopy is that it allows the user to observe living
specimens that are usually destroyed by staining or fixing reagents. The phase turret control has five positions; one for
standard brightfield illumination and four different annuli positions for phase contrast illumination.
COMPONENTS
Plan 10X Ph/0.25 phase din objective.
Plan 20X Ph/0.40 phase din objective.
Plan 40X Ph/0.65 phase din objective.
Plan 100X Ph/1.25 phase din objective.
Five position 1.25NA Phase turret condenser.
Centering telescoping eyepiece
Green filter, 45mm diameter
Blue filter, 45mm diameter
ASSEMBLY
A.
Install phase turret condenser
1.
Rotate coarse focusing knob to move microscope state platform to its highest position.
2.
Loosen knurled locking screw located on the side of microscope condenser mounting ring.
3.
Insert the phase turret condenser sleeve into condenser mounting ring.
4.
Tighten the knurled locking screw to secure phase turret condenser.
B.
Install filter
1.
Insert filter into filter recess located at top of illuminator lighthouse condenser lens.
a.
Blue filter is utilized for bright field observation.
b.
Green filter is utilized for phase observation.
C.
Install objectives
1.
Rotate coarse focusing knob to move microscope stage platform to its highest position.
2.
Remove objective dust caps from the revolving nosepiece.
3.
Screw objective lenses into nosepiece, making certain to mount them in consecutive order, 10x, 20x, 40x,
and 100x.
OPERATION
A.
Rotate condenser focusing control knob to move phase turret condenser to the top of its travel.
B.
Rotate phase turret annuli control until the letters BF (brightfield) can be seen at
front of phase turret condenser assembly. BF opening must click into locked
position to insure proper centering.
C.
Rotate revolving nosepiece to position 10X Ph/0.25 phase objective into optical path.
D.
Place a standard specimen slide (cover slip up) on top of stage surface.
E.
Adjust microscope focus controls until specimen is in sharp focus.
F.
Remove specimen slide from stage.
G.
Remove eyepiece from eyepiece tube, if binocular version remove one of the two eyepieces.
H.
Install centering telescope eyepiece into eyepiece tube.
I.
Loosen knurled locking screw located on side of centering telescope
eyepiece.
J.
Hold knurled locking screw with one hand, grasp very top of centering
telescope eyepiece with other hand, peer through eyepiece while sliding
sleeve up until the phase ring in the objective is in focus (sleeve is
approximately 1" up from knurled locking screw).
MODEL 926 PHASE CONTRAST SET
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