Biotek PowerWave Operator's Manual page 57

Analyze the Results
For each well, calculate the mean and standard deviation of the five readings.
1.
For each mean calculated in step 1, calculate the allowed deviation using the
2.
repeatability specification for a 96-well plate of ± 1.0% ± 0.005 OD (mean * 0.01
+ 0.005).
For each well, compare the standard deviation calculated in step 1 with the
3.
allowed deviation calculated in step 2. The standard deviation should be less
than the allowed deviation.
Example: Five readings in well A1 of 0.802, 0.802, 0.799, 0.798, and 0.801 will
result in a mean of 0.8004, and a standard deviation of 0.0018. The mean
multiplied by 1.0% (0.8004 * 0.010) = 0.008, which, when added to the 0.005 (0.008
+ 0.005) = 0.013, which is the allowable deviation for well A1. Since the standard
deviation for well A1 is less than 0.013, the reader meets the test criteria.
Calculate the results for Linearity:
4.
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PowerWave Operator's Manual
For each of the three dye concentrations, calculate the mean absorbance
for the wells containing that solution (mean of wells A1 to H2, A3 to H4,
and A5 to H6).
Perform a regression analysis on the data to determine if there is
adequate linearity.
Expected Results: Since it is somewhat difficult to achieve high
pipetting accuracy when conducting linear dilutions, an R-Square value
greater than or equal to 0.990 is considered adequate.
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