Optical Specifications; Optical Performance - Biotek PowerWave Operator's Manual

384-Well Read Timing
Normal Read Mode
Endpoint
Rapid Read Mode
Endpoint
Sweep Read Mode
Endpoint
Kinetics: All three read modes are available in Kinetics mode. Single wavelength
reads are limited to the following minimum times.
66 seconds from A1 to A1 in Normal mode, single wavelength, depending
upon density of solution.
23 seconds from A1 to A1 in Rapid mode, single wavelength.
11 seconds from A1 to A1 in Sweep mode, single wavelength.

Optical Specifications

λ range:
λ accuracy:
λ repeatability: ± 0.2 nm
λ bandpass:

Optical Performance

Flat- and round-bottom full-well plates
Absorbance Measurement Range: 0.000 to 4.000 OD
Accuracy:
0.000 to 2.000 OD ± 1.0% ± 0.010 OD Normal and Rapid modes, all plates
2.000 to 2.500 OD ± 3.0% ± 0.010 OD Normal and Rapid modes, all plates
2.500 to 3.000 OD ± 3.0% ± 0.010 OD Normal 96-well plates only
0.000 to 1.000 OD ± 1.0% ± 0.010 OD Sweep mode, all plates
Linearity:
0.000 to 2.000 OD ± 1.0% Normal and Rapid modes, 96-well plates
0.000 to 2.000 OD ± 2.0% Normal and Rapid modes, 384-well plates
2.000 to 2.500 OD ± 3.0% Normal and Rapid modes, all plates
2.500 to 3.000 OD ± 3.0% Normal mode, 96-well plates only
0.000 to 1.000 OD ± 1.0% Sweep mode, all plates
PowerWave Operator's Manual
630 nm
Single
32 to 67 sec.
Single
28 sec.
Single
17 sec.
200 to 999 nm (PowerWave HT)
340 to 999 nm (PowerWave 340)
± 2 nm
5 nm
Specifications |
630/450 nm
Dual
57 to 129 sec.
Dual
49 to 51 sec.
Dual
28 sec.
21