Absorbance Liquid Test 3 (Optional) - Biotek PowerWave Operator's Manual

If any set of well values is out of the expected range, review the other three
sets for the same channel pair. Thus, if A1 and H12 are not within range of
each other, review the compliance of H1 to A12, A2 to H11, and H2 to A11.
This will confirm that there is a problem in one of the eight read channels,
or indicate that the result of one set of wells was in error. If any two sets of
well values for a channel pair are out of the allowed accuracy range, there
may be contamination on, or a problem with, one of the lenses.
If the four corner wells are within the accuracy range, the reader is also in
4.
alignment.

Absorbance Liquid Test 3 (Optional)

Materials
A new 96-well, flat-bottom microplate (Corning Costar
•
recommended)
Calibrated hand pipette(s)
•
Beakers and graduated cylinder
•
Precision balance with a readability of 0.01 g
•
Buffer solution as described below
•
Buffer Solution
Deionized water
•
Phosphate-buffered saline (PBS), pH 7.2-7.6, Sigma
•
equivalent)
β-NADH Powder (β-Nicotinamide Adenine Dinucleotide, Reduced Form)
•
Sigma bulk catalog number N 8129, or preweighed 10-mg vials, Sigma
number N6785-10VL (or BioTek PN 98233). Store the powder according to
the guidelines on its packaging.
Prepare a PBS solution using Sigma
1.
In a beaker, mix 50 mL of the PBS solution with 10 mg of the β-NADH
2.
powder and mix thoroughly. This is the
PowerWave Operator's Manual
#3590 is
®
tablets #P4417 (or
®
tablets.
®
100% Test Solution
53
Liquid Testing |
.