Biotek PowerWave Operator's Manual
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Biotek PowerWave Operator's Manual

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Summary of Contents for Biotek PowerWave

  • Page 1 Microplate Spectrophotometer PowerWave ™ Operator’s Manual...
  • Page 3 PowerWave Microplate Spectrophotometer Operator's Manual June 2011 © 2011 Part Number 7281000 Revision F ® BioTek Instruments, Inc.

  • Page 4: Preface

    BioTek Instruments, Inc. Changes made to the information in this document will be incorporated in new editions of the publication. No responsibility is assumed by BioTek for the use or reliability of software or equipment that is not supplied by BioTek or its affiliated dealers.

  • Page 5: Table Of Contents

    Speed of Reading ..............20 Optical Specifications ............. 21 Optical Performance .............. 21 Hardware and Environmental Specifications ......22 Product Support & Service ............23 Contacting the Technical Assistance Center ......23 Returning Instruments for Service/Repair ......... 23 PowerWave Operator’s Manual...
  • Page 6 7: Turn on the Reader ..............29 Operational/Performance Qualification .......... 30 Repackaging and Shipping Instructions ......... 31 Chapter 3: Operation ..............35 Operating the PowerWave ............36 Getting Started with Gen5 ............36 Recommendations for Optimum Performance......... 37 Where to Go Next ..............38 Chapter 4: Instrument Qualification ..........

  • Page 7: Contact Information

    802-655-4740 (outside the U.S.) Fax: 802-654-0638 E-Mail: tac@biotek.com European Coordination Center/Authorized European Representative BioTek Instruments GmbH ® Kocherwaldstrasse 34 D-74177 Bad Friedrichshall Germany Internet: www.biotek.de Phone: +49 (0) 7136 9680 Fax: +49 (0) 7136 968 111 E-Mail: info@biotek.de PowerWave Operator’s Manual...

  • Page 8: Revision History

    Included references to KCjunior as an additional primary operating software and substitute for KC4. Included information about the compatibility of the PowerWave with the Bio- Stack™ Automated Microplate Stacking System, and brief paragraphs concerning installation, serial cable connections, error codes, and alignment when operating the PowerWave with the Bio-Stack;...
  • Page 9 Liquid Tests 1, 2, or 3. Changed ‘Universal’ to ‘Absorbance’ in ‘Universal Test Plate’ and ‘Universal Plate Test’. In Liquid Test 1, added BioTek wetting agent (7773002) to list of ingredients. In Liquid Test 3, changed Sigma® ‘P 3563 packets’ to ‘PBS tablets (#4417, or equivalent).’...

  • Page 10: Document Conventions

    The user must evaluate this instrument with PC-based software in conjunction with the specific assay. This evaluation must include the confirmation that performance characteristics for the specific assay are met. BioTek Gen5 software package provides the user with instrument control and data • reduction capabilities.

  • Page 11: Quality Control

    Warranty and Product Registration Review the Warranty information that shipped with your product. Register your product(s) with BioTek to ensure that you receive important information and updates. Contact the Customer Resource Center (CRC) at www.biotek.com or by calling 888/451- 5171 or 802/655-4740.

  • Page 12: Hazards

    . Some assays or specimens may pose a Warning! Potential Biohazards biohazard. Adequate safety precautions should be taken as outlined in the assay’s package insert. Always wear safety glasses and appropriate protective equipment, such as chemically resistant rubber gloves and an apron. BioTek Instruments, Inc.

  • Page 13: Precautions

    Precautions | Precautions The following precautions are provided to help avoid damage to the instrument: . The instrument should be serviced by BioTek authorized service Caution: Service personnel. Only qualified technical personnel should perform troubleshooting and service procedures on internal components.

  • Page 14: Ce Mark

    Directive 2002/96/EC: Waste Electrical and Electronic Equipment Disposal Notice: This instrument contains printed circuit boards and wiring with lead solder. Dispose of the instrument according to Directive 2002/96/EC, “on waste electrical and electronic equipment (WEEE)” or local ordinances. BioTek Instruments, Inc.

  • Page 15: Directive 98/79/Ec: In Vitro Diagnostics

    Communications. Le present appareil numerique n'emet pas de bruits radioelectriques depassant les limites applicables aux appareils numerique de la Class A prescrites dans le Reglement sur le brouillage radioelectrique edicte par le ministere des Communications du Canada. PowerWave Operator’s Manual...

  • Page 16: User Safety

    • UL 61010-1, “Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use, Part 1: General Requirements” International • EN 61010-1, “Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use, Part 1: General Requirements” BioTek Instruments, Inc.

  • Page 17: Safety Symbols

    Attenzione, rischio di schiacciare ed intrappolarsi Warning, hot surface Warning, potential biohazards Attention, surface chaude Attention, risques biologiques potentiels Warnen, heiße Oberfläche Warnung! Moegliche biologische Giftstoffe Precaución, superficie caliente Atención, riesgos biológicos Attenzione, superficie calda Attenzione, rischio biologico PowerWave Operator’s Manual...
  • Page 18 Recogida selectiva de aparatos eléctricos y electrónicos Dispositivo medico diagnostico in vitro Raccolta separata delle apparecchiature elettriche ed elettroniche Consult instructions for use Consulter la notice d’emploi Gebrauchsanweisung beachten Consultar las instrucciones de Consultare le istruzioni per BioTek Instruments, Inc.

  • Page 19: Chaper 1: Introduction

    Chapter 1 Introduction This chapter introduces the PowerWave Microplate Spectrophotometer, describes its features and specifications, and provides contact information for technical assistance. Product Description ..............18 Package Contents ..............18 Optional Accessories ..............19 Specifications ................20 Product Support and Service ............. 23...

  • Page 20: Product Description

    • Robot accessible carrier. Compatible with BioTek’s BioStack Microplate Stacker.  If you purchased the BioStack to operate with the PowerWave, refer to the BioStack Operator’s Manual for installation, setup, and operation instructions. If you are interested in purchasing the BioStack, contact your local BioTek dealer or visit www.biotek.com.

  • Page 21: Optional Accessories

    PowerWave Product Qualification (IQ-OQ-PQ) package (7280520) • Liquid Test Solutions:  BioTek Wetting Agent (PN 7773002)  BioTek QC Check Solution No. 1 (PN 7120779 for 25 ml; PN 7120782 for 125 ml) BioStack Microplate Stacker (contact Customer Care) • PowerWave Operator’s Manual...

  • Page 22: Specifications

    Microplates • All models accommodate standard 96-well microplates, and up to 8 BioCells. • The PowerWave HT also accommodates standard 384-well microplates. Speed of Reading The plate read time and accuracy are dependent on the method of reading: • Normal mode is the slowest of the three available modes. After positioning the well over the beam, the instrument waits 100 milliseconds before taking the measurement (8-flash data collection).

  • Page 23: Optical Specifications

    23 seconds from A1 to A1 in Rapid mode, single wavelength. 11 seconds from A1 to A1 in Sweep mode, single wavelength. Optical Specifications λ range: 200 to 999 nm (PowerWave HT) 340 to 999 nm (PowerWave 340) λ accuracy: ± 2 nm λ...

  • Page 24: Hardware And Environmental Specifications

    24-volt external power supply compatible with 100-240 V~ ± 10% @50-60 Hz Power Consumption: 100 VA max Temperature Control: 4°C over ambient to 50°C Temperature Variation: ± 0.5C across the plate @ 37°C (250 µL per well with the plate sealed) BioTek Instruments, Inc.

  • Page 25: Product Support & Service

    If your instrument(s) or software fails to function properly, if you have questions about how to use or maintain your products, or if you need to send an instrument to BioTek for service or repair, please contact our Technical Assistance Center (TAC).
  • Page 26 | Chapter 1: Introduction BioTek Instruments, Inc.

  • Page 27: Chapter 2: Installation

    Chapter 2 Installation This chapter provides instructions for unpacking and setting up the PowerWave. Product Registration ..............26 1: Unpack and Inspect the Instrument ........26 2: Remove the Carrier Shipping Bracket ........26 3: Select an Appropriate Location ..........27 4: Connect the Power Supply ............

  • Page 28: Product Registration

    The manufacturer will arrange for repair or replacement of your reader. See Preparing the PowerWave for Shipment at the end of this chapter for repacking and shipping instructions. 2: Remove the Carrier Shipping Bracket ...

  • Page 29: 3: Select An Appropriate Location

    Strong light can reduce the linear performance range • Excessive ambient light: of the reader. Optical density readings may be affected by extraneous particles in the • Dust: microplate wells. A clean work area is necessary to ensure accurate readings. PowerWave Operator’s Manual...

  • Page 30: 4: Connect The Power Supply

    Locate the power inlet on the back of the reader. Insert the power supply’s plug into the reader’s inlet. Tighten the plug barrel. Plug the cord into an appropriate power receptacle. Serial port Power inlet Figure 2: Serial port and power supply inlet BioTek Instruments, Inc.

  • Page 31: 5: Connect The Host Computer

    2. Connect one end of the supplied serial cable to an appropriate port on the computer.  BioTek offers a USB to Serial Adapter (PN 75104) to connect the serial cable to a USB port on your computer. Contact BioTek Customer Care.

  • Page 32: Operational/Performance Qualification

    Figure 3: Power on/off switch and carrier eject button Operational/Performance Qualification Your PowerWave Microplate Spectrophotometer was fully tested at BioTek prior to shipment and should operate properly following the successful completion of the installation and setup procedures described throughout this chapter.

  • Page 33: Repackaging And Shipping Instructions

    BioTek if you have misplaced the microplate carrier shipping bracket (PN 7282014) or mounting screws (3, PN 19186). If you need to ship the reader to BioTek for service or repair, be sure to use the original packaging materials. Other forms of commercially...
  • Page 34 Place the power supply, cord, and communication cables into the accessories box. Close the top of the box and secure it with shipping tape. When finished, write the RMA number on the outside of the box and ship the box to BioTek. BioTek Instruments, Inc.
  • Page 35 Repackaging and Shipping Instructions | Plastic bag Bottom foam caps Top foam caps (2) Power cord Figure 4: Packing the PowerWave PowerWave Operator’s Manual...
  • Page 36 | Chapter 2: Installation BioTek Instruments, Inc.

  • Page 37: Chapter 3: Operation

    Chapter 3 Operation This chapter briefly describes how to use BioTek’s Gen5 software to operate the PowerWave. It also contains recommendations for achieving optimum performance. Operating the PowerWave ............36 Getting Started with Gen5............38 Recommendations for Optimum Performance ......37...

  • Page 38: Operating The Powerwave

    | Chapter 3: Operation Operating the PowerWave Most users will operate the PowerWave using BioTek’s Gen5 software. If you have not already done so, please follow the instructions in Chapter 2 and the Gen5 Getting Started Guide for connecting the host computer and installing Gen5.

  • Page 39: Recommendations For Optimum Performance

    • Although the PowerWave supports most flat, U-bottom, and V bottom microplates, the reader achieves optimum performance with optically clear, flat-bottomed wells. Non uniformity in the optical density of the well bottoms can cause loss of •...

  • Page 40: Where To Go Next

    Refer to the Gen5 Getting Started Guide and help system to learn more about using Gen5 to operate the PowerWave. Review the remaining chapters in this Operator’s Manual to learn how to test the • performance of the PowerWave, clean and maintain the reader, and troubleshoot problems. BioTek Instruments, Inc.

  • Page 41: Chapter 4: Instrument Qualification

    Chapter 4 Instrument Qualification This chapter discusses procedures for qualifying the reader’s initial and ongoing performance. Recommended Qualification Schedule ......... 40 Qualification Procedures ............41 System Test ................41 Absorbance Plate Test .............. 44 Liquid Testing ................47...

  • Page 42: Overview

    Operational Qualification (OQ), and Performance Qualification (PQ) procedures for the PowerWave Microplate Spectrophotometer. Every PowerWave reader is fully tested at BioTek prior to shipment and should operate properly upon initial setup. If you suspect that a problem occurred during shipment, if...

  • Page 43: Qualification Procedures

    System Test The PowerWave automatically runs the System Test each time it is powered on. The test can also be run (and a report generated) using Gen5. The System Test report should be printed to document periodic testing and for troubleshooting purposes. See a sample System Test report on page 42.

  • Page 44: Sample System Test Report

    Lambda: 999 Gain: 5.33 Resets: 1 Channel: Air: 19977 34186 39376 35131 37500 36779 32458 36029 35249 Dark: 9900 10050 10095 9849 10010 9888 9934 9831 10004 Delta: 10077 24136 29281 25282 27490 26891 22524 26198 25245 BioTek Instruments, Inc.
  • Page 45 AUTOCAL is not included in the SYSTEM TEST result given below. Middle Sensor: x= 13852 Tested: 13852 Delta: SYSTEM TEST PASS The SYSTEM TEST result will be either PASS or FAIL and refers only to the optics portion of the test. PowerWave Operator’s Manual...

  • Page 46: Absorbance Plate Test

    See page 50. Test Plate and Certificates To run the Absorbance Plate Test, you will need BioTek's Absorbance Test Plate (PN 7260522), with its accompanying certificates. contains standard OD values for the filters at •...

  • Page 47: Sample Absorbance Plate Test Report

    PASS PASS PASS PASS PASS PASS Spectral Scan Results 580nm= 1.933 586nm= 2.892 581nm= 1.956 587nm= 2.753 582nm= 2.059 588nm= 2.516 583nm= 2.253 589nm= 2.334 584nm= 2.511 590nm= 2.186 585nm= 2.785 Test Plate Standard=587nm Calculated Peak=586nm PASS PowerWave Operator’s Manual...

  • Page 48: Test Results

    For example, if the test range is 580 to 590 nm, the Certificate value is 587 nm, and the reader reports a peak value of 590 nm, then the reader meets specifications. If the reader reports 591 nm, then the reader does not meet specifications. BioTek Instruments, Inc.

  • Page 49: Liquid Testing

    If you must test the reader’s performance at 340 nm, perform Liquid Test 3. • BioTek offers a dye solution (PN 7120779, 25 mL; or 7120782, 125 mL) that may be • used in the stock solution formulation for Liquid Tests 1 and 2, or, if you prefer, you may use Solution A described on the next page.

  • Page 50: Absorbance Liquid Test 1

    Stock Solution A or B, which may be formulated by diluting a dye solution • available from BioTek (A) or from the ingredients listed below (B). Solution A BioTek QC Check Solution No. 1 (PN 7120779, 25 mL; PN 7120782, 125 mL) • Deionized water •...
  • Page 51 For Example: If the mean value for well A1 in the Normal position is 1.902, where the specified accuracy is ± 1.0% ± 0.010 OD, then the expected range for the PowerWave Operator’s Manual...

  • Page 52: Absorbance Liquid Test 2

    80% in the third tube, all the way to 10% in the tenth tube. Dilute using the 0.05% solution of deionized water and Tween ® This solution can also be made by diluting the BioTek wetting agent 200:1. BioTek Instruments, Inc.
  • Page 53 Print out the five sets of Delta OD data, or export them to an Excel spreadsheet. Calculate the results for Linearity: • Calculate the mean absorbance for each well, and average the means for each concentration. • Perform a regression analysis on the data to determine if there is adequate linearity. PowerWave Operator’s Manual...
  • Page 54 ± 1.0% ± 0.010 OD, then the expected range for the mean of the same well in the H12 position is 1.873 to 1.931 OD. (1.902 * 1% = 0.019 + 0.010 = 0.029, which is added and subtracted from 1.902 for the range.) BioTek Instruments, Inc.

  • Page 55: Absorbance Liquid Test 3 (Optional)

    β-NADH Powder (β-Nicotinamide Adenine Dinucleotide, Reduced Form) • Sigma bulk catalog number N 8129, or preweighed 10-mg vials, Sigma number N6785-10VL (or BioTek PN 98233). Store the powder according to the guidelines on its packaging. Prepare a PBS solution using Sigma tablets.
  • Page 56 Read the Plate Read the microplate five times using Normal mode, single wavelength at 340 nm, no blanking (or blank on air). Print the five sets of raw data or export it to an Excel spreadsheet using Gen5. BioTek Instruments, Inc.
  • Page 57 Perform a regression analysis on the data to determine if there is adequate linearity. Expected Results: Since it is somewhat difficult to achieve high pipetting accuracy when conducting linear dilutions, an R-Square value greater than or equal to 0.990 is considered adequate. PowerWave Operator’s Manual...
  • Page 58 | Chapter 4: Instrument Qualification BioTek Instruments, Inc.

  • Page 59: Chapter 5: Decontamination

    Chapter 5 Decontamination This chapter contains procedures for decontaminating and cleaning the PowerWave. Purpose .................. 58 Clean Plate Carrier and Exposed Surfaces ........58 Decontamination ..............59...

  • Page 60: Purpose

    Centers for Disease Control and Prevention (CDC). Neither BioTek nor the CDC assumes any liability for the adequacy of these solutions and methods. Each laboratory must ensure that decontamination procedures are adequate for the Biohazard(s) they handle.

  • Page 61: Clean Plate Carrier And Exposed Surfaces

    • Mild detergent (optional) • Turn on the PowerWave and press the carrier eject button to eject the microplate carrier. Turn off and unplug the reader from the power supply. Moisten a clean, lint-free cloth with water, or with water and the mild detergent.

  • Page 62: Decontamination

    Warning! protection when handling the solution. Turn on the PowerWave and press the carrier eject button to eject the carrier. Turn off and unplug the instrument from the power supply. Prepare an aqueous solution of 0.5% sodium hypochlorite (NaClO, or bleach).

  • Page 63: Chapter 6: Troubleshooting

    Chapter 6 Troubleshooting This chapter lists error codes that may appear during operation of the PowerWave, and provides troubleshooting tips. Error Codes ................62 General Errors ................. 63 Fatal Errors (A###) ..............67...

  • Page 64: Error Codes

    System > Diagnostics > Run System Test Error Codes During Operation with the BioStack Error codes may appear in Gen5 during operation of the PowerWave with the BioStack Microplate Stacker. Gen5 error codes display in a negative value format, for example: -8, -100.

  • Page 65: General Errors

    Order sorting (bandpass) filter wheel failed positional verify. • Filter wheel obstructed by motor gear. • Motor gear loose on motor shaft. 0402 Carrier y-axis failed positional verify. • Dirty rail or dry bearings are causing too much friction. PowerWave Operator’s Manual...
  • Page 66 • The photo-detector is not connected or is defective, yielding a noise reading of zero. • The photo-detector is too noisy and is defective. Channel dark range is < 100 or > 20000 during calibration, or < 100 during a 0901- 0908 filter test. BioTek Instruments, Inc.
  • Page 67 The instrument calibration values are not loaded into the flash memory. • The PCB was changed and the flash memory does not have the calibration values loaded. • Failure in Main PCB memory. Contact BioTek TAC for more information. 1201- Lambda table calibration data missing from reader.
  • Page 68 This error is typically used only for software development purposes. If it occurs, however, try turning the instrument off and then on again after a wait of 30 seconds. If the error persists, contact BioTek Technical Support. A/D calibration standby signal on the analog board never went low, or A/D 1C00 calibration standby line went low but never transitioned to a high.

  • Page 69: Fatal Errors

    This error indicates there is a failure with the absorbance analog board or the cable to the PCB is defective. Fatal Errors Fatal errors indicate conditions that require immediate attention. If a fatal error is displayed, contact BioTek’s Technical Assistance Center for further instructions. Code Description A100 Task control block not available.
  • Page 70 | Chapter 6: Troubleshooting BioTek Instruments, Inc.

  • Page 71: Chapter 7: Instrument Dimensions

    Chapter 7 Instrument Dimensions This chapter contains the PowerWave’s dimensions, for use with robotic interfaces.

  • Page 72: Instrument Dimensions

    Instrument Dimensions The figure below shows the location of the microplate carrier in reference to the exterior surfaces of the PowerWave, and the mounting holes on the bottom of the reader. This should facilitate system setup with a robotic unit.

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