Protocol - NEC express5800 A2010b User Manual

PrimeScript™ RT Master Mix （Perfect Real Time）
VII. Protocol
Reverse transcription reaction
For RNA preparation method, please see Section X.
1. Prepare the reverse-transcription reaction solution on ice.
<Per reaction>
Reagent
5X PrimeScript RT Master Mix (Perfect Real Time)
total RNA
RNase Free dH
＊ : The scale of the reverse-transcription reaction can be increased as necessary.
2. Perform the reverse-transcription reaction after gently mixing the reaction
solution.
37℃ 15 min. (reverse-transcription)
85℃
4℃
Note: When the reverse-transcription solution obtained in Step B is to be
URL:http://www.takara-bio.com
O
2
Reverse transcription of as much as 500 ng of total RNA is possible with 10 μl
of reaction solution.
5 sec. (for heat inactivation of reverse transcriptase)
introduced to a real-time PCR reaction, the volume of the solution
should be 10% or less of the reaction volume for real-time PCR.
Cat. #RR036Q
v201204Da_2
Amount Final conc.
2 μl
＊
up to 10 μl
1X
5