Description; Components - NEC express5800 A2010b User Manual

PrimeScript™ RT Master Mix （Perfect Real Time）
I.

Description

This product is a reverse transcription reagent kit designed to perform reverse
transcription optimized for 2 step real-time RT-PCR (RT-qPCR). It contains a 5X pre-
mixed reagent containing all of the components needed for quantitative RT-PCR
reverse transcription (PrimeScript RTase, RNase Inhibitor, Random 6mers, Oligo dT
Primer, dNTP Mixture, and reaction buffer), and a reaction can be started simply by
adding template RNA and water. Because it uses PrimeScript RTase, which features
excellent extendibility, this product, like PrimeScript RT reagent Kit (Perfect Real Time)
(Cat. #RR037A/B), makes it possible to synthesize template cDNA for real-time PCR
efficiently in a short time.
The cDNA obtained with this product can be used in both the SYBR® Green Assay and
TaqMan® Probe Assay. Please use this product in combination with quantitative PCR
reagents, such as SYBR® Premix Ex Taq II (Tli RNaseH Plus) and Premix Ex Taq (Probe
qPCR), depending upon your objectives.
Note: TAKARA BIO is under a license agreement with Molecular Probes Inc. for the
use of SYBR® Green I as a reagent for research purposes. SYBR® is a registered
trademark of Molecular Probes Inc.
II. Components (for 20 x 10 μl reactions)
1．5X PrimeScript RT Master Mix (Perfect Real Time)
2．RNase Free dH
3．EASY Dilution (for Real Time PCR)
＊ 1:
＊ 2:
URL:http://www.takara-bio.com
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Contains PrimeScript RTase, RNase Inhibitor, Oligo dT Primer, Random
6mers, dNTP Mixture, and reaction buffer (containing Mg
To be used when preparing serial dilutions of total RNA and cDNA. EASY
Dilution makes it possible to obtain a precise dilution down to very low
concentrations. Moreover, this solution will not affect reverse-transcription
and PCR reactivity. You can use the diluted solution directly in reverse-
transcription and PCR reactions.
EASY Dilution (for Real Time PCR) may also be purchased separately
(Cat. #9160).
Note: Please use EASY Dilution in combination with Takara Bio's Real-
Time PCR Reagents. Compatibility with products from other
manufacturers has not yet been verified.
Cat. #RR036Q
v201204Da_2
＊1
＊2
40 μl
1 ml
1 ml
2+
).
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