Troubleshooting Guide - Olimpus CKX41 Instruction Manual

Culture microscopes
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TROUBLESHOOTING GUIDE

Under certain conditions, performance of the unit may be adversely affected by factors other than defects. If problems occur,
please review the following list and take remedial action as needed. If you cannot solve the problem after checking the
entire list, please contact Olympus for assistance.
Trouble
1. Optical System
a) Although the illumination is on, the
field of view is dark.
b) The edge of the field of view is ob-
scured or not evenly illuminated.
c) Dirt or dust is visible in the field of
view.
d) The image glares.
e) Visibility is poor.
· Image is not sharp.
· Contrast is poor.
· Details are indistinct.
· Phase contrast effect cannot be ob-
tained.
Cause
The socket pin is not connected to the
illumination column.
The bulb is burned out.
The light intensity control is set too low.
Too many filters are stacked.
The mounted bulb is not the one desig-
nated.
The revolving nosepiece is not correctly
engaged.
The filter is stopped halfway.
The phase slider is not engaged properly. Move the slider until it clicks into place.
Dirt/dust on the specimen.
Dirt/dust on the eyepieces.
The aperture iris diaphragm is stopped
down too far.
The objective is not correctly engaged in
the light path.
The aperture iris diaphragm is opened
or stopped down too far in brightfield ob-
servation.
Correction collar on correction collar
equipped objective is not properly adjusted.
A lens (condenser, objective, eyepiece or
culture vessel) is dirty.
The bottom of the culture vessel exceeds
2.5 mm in phase contrast observation.
You are using a brightfield objective.
The light annulus of the condenser does not
match the phase annulus of the objective.
The light annulus and phase annulus are
not centered.
The objective in use is not compatible
with phase contrast observation.
When the edge of the culture vessel is
viewed, the phase annulus and light an-
nulus deviate from one another.
CKX41/CKX31
Remedy
Connect it securely.
Replace it with a new one.
Set it to the appropriate position.
Reduce them to the minimum required
number.
Use the designated 6 V, 30 W halogen
bulb.
Make sure that the revolving nosepiece
clicks properly into place.
Push it in all the way.
Replace it with a clean specimen.
Clean them thoroughly.
Open it.
Turn the revolving nosepiece until it
clicks properly into place.
Adjust the aperture properly.
While focusing, turn the correction
collar to find the best position.
Clean it thoroughly.
Use a culture vessel with bottom thick-
ness of no more than 2.5 mm.
Use a phase contrast objective.
Adjust the light annulus so that it matches
the phase annulus of the objective.
When using the IX2-SL, center them
correctly.
Use an objective applicable to phase
contrast observation.
Move the vessel until phase contrast
effect is achieved. Also remove the
slider and set the aperture iris dia-
phragm lever to "
".
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