Olimpus FLUOVIEW FV500 Manuals

Manuals and User Guides for Olimpus FLUOVIEW FV500. We have 1 Olimpus FLUOVIEW FV500 manual available for free PDF download: User Manual

Olimpus FLUOVIEW FV500 User Manual (470 pages)

CONFOCAL LASER SCANNING BIOLOGICAL MICROSCOPE

Brand: Olimpus | Category: Microscope | Size: 15 MB

1 System Overview 1-1

9
- Table of Contents
  9
- System Overview
  11
1 Getting Started Fluoview 1-1

25
- Getting Started FLUOVIEW
  
  33
  - Basic Operations
    33
    
    Microscope
    33
    
    General Mouse Operation Procedures
    45
    
    Names of Major Panel and Window Controls and Their Functions
    46
  - Outline of LSM Observation Procedures
    48
    
    Turning Power on
    50
    
    Starting the Software
    54
    
    Combination with BX
    56
    
    Focusing on the Specimen
    56
    
    Combination with AX
    58
    
    Combination with IX
    60
    
    Combination of IXBP (Bottom Port)
    61
    
    Combination with Upright Microscope (BX)
    63
    
    Setting the LSM Light Path
    63
    
    Combination with Inverted Microscope (BX50WI)
    65
    
    Combination with Upright Microscope (AX)
    67
    
    Combination with Upright Microscope (AX70A)
    69
    
    Combination with Inverted Microscope (IX)
    71
    
    Combination of IXBP (Bottom Port)
    73
    
    Selecting the Dyeing Method
    75
    
    Selecting the Filters
    78
    
    Selecting the ND Filters
    80
    
    Setting the Objective Magnification
    81
    
    Setting the Observation Condition
    81
    
    Setting the Channels
    82
    
    Setting the Highest Scan Speed
    82
    
    Setting the Zoom Ratio to 1X
    82
    
    Setting the XY Observation Mode
    83
    
    Repeated Scanning Operation
    84
    
    Setting the Cross-Section to be Observed
    84
    
    Setting the Area to be Observed
    85
    
    Setting a Lower Scan Speed
    86
    
    Stopping Repeated Scanning
    86
    
    Acquiring Image
    87
    
    Saving Image
    87
    
    Exiting from the Software
    89
    
    Turning Power off
    90
  - Online Help
    92
    
    Function Help
    92
    
    Microscope Help
    93
    
    Configuring the Microscope
    96
    
    Configuring the Microscope (Combination with BX51, BX61)
    98
    
    Configuring the Microscope (AX70A)
    102
    
    Configuring the Filters
    104
    
    Setting the C.A. Diameters
    106
- Applied Operations
  
  111
  - General Operation Procedure
    111
    
    Image Acquisition Procedure (Section (A))
    113
    
    Image Acquisition Procedure in an Observation Mode (Section (B))
    114
    
    Examples of Operation Procedures
    115
  - Image Acquisition
    117
    
    Image Acquisition in XY Observation Mode
    118
    
    Configuring the Microscope
    119
    
    Setting the Filters
    125
    
    Setting the ND Filters
    127
    
    Setting the Observation Condition
    128
    
    Acquiring Image
    136
    
    Acquiring Image in Accumulation Mode
    137
    
    Saving the Acquired Image in File
    140
    
    Image Acquisition in Other Observation Modes
    141
    
    XZ Observation Mode
    141
    
    XT Observation Mode
    146
    
    XZT Observation Mode
    148
    
    XYZ Observation Mode
    155
    
    XYT Observation Mode
    161
    
    XYZT Observation Mode
    164
    
    Differences in Image Acquisition Method between Fluorescent and Transmitted Images
    170
    
    Monochrome Image
    170
    
    Dual-Fluorochrome Image
    173
    
    Transmitted Image
    176
    
    Acquiring Multiple Images (XYZ, XYT or XYZT Observation)
    180
    
    High-Speed Image Acquisition
    181
    
    Acquiring Finer Image (Sequential Scan)
    182
    
    Image Acquisition of Rectangular Position at Desired Angle
    185
    
    Image Acquisition of a Line at Desired Angle
    187
    
    Display the Change of Image Intensity (Point Scan)
    188
    
    Image Acquisition on Desired Line (XZ, XT or XZT Observation)
    193
    
    Image Acquisition in the Laser Excitation Mode
    196
    
    Making REX Mask File
    197
    
    Example of FRAP Experiment
    202
  - Saving, Opening and Shredding Images
    211
    
    Saving Images
    214
    
    Saving Images as a Series
    214
    
    Saving a Display
    216
    
    Saving Specified Area of Image
    218
    
    Saving Animation Images
    221
    
    File Types Available for Save
    222
    
    Opening Previously Saved Images
    229
    
    Shredding Images
    229
    
    Saving Comment Together with Image
    231
    
    Checking the Image Information/Acquisition Parameters
    233
    
    Saving the Image Information/Observation Condition
    237
    
    Saving the Region File
    240
    
    Saving/Reading the Region File
    240
    
    Reading the Region File
    242
  - Changing the Image Display Method
    245
    
    Displaying an Image in Simulated Colors
    245
    
    Editing the LUT (Look up Table)
    247
    
    LUT Graph Editing According to Colors
    247
    
    LUT Graph Editing by Gamma Correction
    249
  - Switching the Displayed Channels (Ch1 - Ch5)
    250
  - Displaying Images of Multiple Channels Simultaneously
    251
    
    Over and under Views, Single View)
    251
    
    Displaying Images Separately Per Channel
    252
    
    Views)
    252
    
    Displaying Merged Image of Multiple Channels (Single View)
    253
  - Changing the Number of Divided Images
    254
    
    Increasing the Number of Divided Images
    254
    
    Decreasing the Number of Divided Images
    256
  - Switching the Display Method of Multiple Images
    258
  - Displaying Multiple Image Slices Together
    259
    
    Displaying Multiple Images Per Channel
    260
    
    Displaying Images of Two Channels Together
    262
    
    Displaying Time-Lapse Images
    263
    
    Displaying Multiple Multiple Sections Images
    264
    
    Displaying same Images in Different Display Methods
    264
    
    Displaying Different Images Together
    266
    
    Re-Arranging Images Using the same Display Method
    266
  - Displaying an Image in Full Screen
    269
  - Magnifying/Reducing an Image
    271
  - Image Processing
    272
    
    Filtering
    272
    
    Contour Enhancement
    273
    
    Noise Reduction
    276
    
    Image Sharpening
    277
    
    DIC Correcting DIC Level Irregularities
    278
    
    Contrast Conversion
    280
    
    Image Addition
    283
    
    Mathematical Operations between Images
    283
    
    Image Division
    286
    
    Image Multiplication
    286
    
    Image Subtraction
    286
    
    NOT Image
    287
    
    Image and Image
    289
    
    Image or Image
    291
    
    Image XOR Image
    292
    
    Image Analysis
    293
    
    Checking the Intensity of a Specific Part
    294
    
    Intensity Values on a Line (Line Profile)
    294
    
    Intensity Values on a Planar Region (Bird's Eye View)
    297
    
    Checking the Intensity Distribution of a Specific Part
    302
    
    Intensity Distribution on a Line (Histogram)
    302
    
    Intensity Distribution on a Planar Region (Histogram)
    303
    
    Area Measurement
    305
    
    Length Measurement
    305
    
    Measuring a Part
    305
    
    Measuring the Change in Mean Value of Intensity
    306
    
    Measuring the Change in Integrated Intensity
    312
    
    Building an Image from a Different Viewpoint
    314
    
    Building Extended Focus Image from XYZ Image
    314
    
    Display Switching to Built Image
    314
    
    Turning Built Image into Single Image
    317
    
    Building Line Images to be Viewed in Z Direction
    319
    
    Turning Built Image into Time Series Image
    319
    
    Viewing 3D Image
    321
    
    Successive Display of Images
    322
    
    Changing the Successive Display Speed
    323
    
    Animation
    324
    
    Building Stereo 3D Images
    326
    
    Building a 3D Image to be Viewed through Color (Red/Green) Eyeglasses2-218
    328
    
    Displaying Images Successively
    330
    
    Displaying Images Together
    330
    
    Viewing Images Following the Progress of Time
    330
    
    Transferring Analysis Data to Another Application
    333
    
    Transferring Data to Another Application
    333
    
    Transferring the Plot Image of Analysis Data to Another Application
    336
    
    Transferring Image Data to Another Application (Microvoxel, Paint, Etc.)2-227
    337
    
    Entering Comment in Image
    338
    
    Writing Characters in Image
    338
    
    Displaying the Image Intensity
    340
    
    Displaying the X-Coordinate/Y-Coordinate of the Image
    341
    
    Drawing a Figure in Image
    342
    
    Drawing a Scale in Image
    343
    
    Drawing an Arrow in Image
    345
    
    Drawing Color Bars in Image
    346
    
    Deleting Comment
    347
    
    Moving Comment
    347
    
    Changing the Comment Size
    349
    
    Changing the Comment Color
    350
    
    Changing the Comment Font
    350
    
    Image Output at Printer
    352
    
    Merger/Extraction of Image Channels
    354
    
    Setting the Range of Multiple Image Slices
    354
    
    Merging Image Channels
    355
    
    Extracting Channels from Image
    359
    
    Changing the Chart Display Method
    363
    
    Chart] Panel
    363
    
    Series] Panel
    371
    
    Pop-Up Menus
    374
- Appendix A List of Tools
  
  379
- Appendix A-1 List of Tools
  
  379
- Appendix A-1-1 Toolbar
  
  379
- Appendix A-1-2 Tools at the Top of Display Panel
  
  380
- Appendix B List of Hot Keys
  
  381
- Appendix C Glossary
  
  385
- Appendix D U-MCB Setting
  
  389
- Appendix D-1 Mirror Cube Data Input(CUBE
  
  390
- Appendix D-2 Objective Data Input(ob
  
  391
- Appendix D-3 Transmitted Light DIC Prism Setting (UCD
  
  393
- Appendix D-4 Setting PC Communications
  
  395
- Appendix E User Registration
  
  397
- Appendix F Formatting of Magnetic Optical Disk F-1
  
  397
- Appendix H USER REGISTRATION of FV500
  
  397
- Appendix H-1 User Registration
  
  397
- Appendix G Converting Analysis Data into a Chart Using EXCEL
  
  403
- Appendix H-2 Logging into the FV500
  
  410
- Appendix H-3 Deleting a User
  
  411
- Appendix I Change of Default Folder for [File I/O] Paneli-1
  
  415
- Appendix J List of Functions in the [Active Overlays] Dialog Box
  
  421
- Appendix J-1 Coordinate Position Data
  
  421
- Appendix J-1-1 X-Coordinate
  
  421
- Appendix J-1-2 Y-Coordinate
  
  422
- Appendix J-1-3 Other
  
  423
  - Position
    423
  - Animation
    423
- Appendix J-2 Intensity Data
  
  424
- Appendix J-3 Other
  
  425
  - Software Setup
    431
    
    Re-Setup or Updating of the Software
    431
    
    New Setup of the Software
    433
    
    Overall Setting of FLUOVIEW
    436
    
    Setting the System Configuration
    436
    
    Setting the [BX Control Panel]
    444
    
    Adding the Dyeing Method
    450
  - Maintenance of Major System Units
    453
    
    Laser Scanning Microscope
    453
    
    Auto Alignment Operation (Remote Switch)
    454
    
    UV-Ar Laser
    454
    
    Checking, Refilling and Replacing Water in Chiller
    455
1 Troubleshooting Guide 1-1

461

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Olimpus FLUOVIEW FV500 Manuals

Olimpus FLUOVIEW FV500 User Manual (470 pages)

Table of Contents

1 System Overview 1-1

1 Getting Started Fluoview 1-1

Getting Started FLUOVIEW

Applied Operations

Appendix A List of Tools

Appendix A-1 List of Tools

Appendix A-1-1 Toolbar

Appendix A-1-2 Tools at the Top of Display Panel

Appendix B List of Hot Keys

Appendix C Glossary

Appendix D U-MCB Setting

Appendix D-1 Mirror Cube Data Input(CUBE

Appendix D-2 Objective Data Input(ob

Appendix D-3 Transmitted Light DIC Prism Setting (UCD

Appendix D-4 Setting PC Communications

Appendix E User Registration

Appendix F Formatting of Magnetic Optical Disk F-1

Appendix H USER REGISTRATION of FV500

Appendix H-1 User Registration

Appendix G Converting Analysis Data into a Chart Using EXCEL

Appendix H-2 Logging into the FV500

Appendix H-3 Deleting a User

Appendix I Change of Default Folder for [File I/O] Paneli-1

Appendix J List of Functions in the [Active Overlays] Dialog Box

Appendix J-1 Coordinate Position Data

Appendix J-1-1 X-Coordinate

Appendix J-1-2 Y-Coordinate

Appendix J-1-3 Other

Appendix J-2 Intensity Data

Appendix J-3 Other

1 Troubleshooting Guide 1-1

10 Image Is Dark and Noisy

11 The Position Reproduction of the Z-Motor Is Poor

13 The Scale of the [Z Stage] Sub-Panel in the [Acquire] Panel Cannot

22 Bios some Check Error Message Appears at the Moment of

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