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4-4 Illumination Column
Aperture iris
diaphragm image
Objective pupil
Fig. 18
@
Fig. 19
1
Using the Filters
}Using appropriate filters according to the purposes allows you to observe
and photograph specimens more effectively. Particularly, the use of the LBD
filter is recommended in observation and photomicrography because it
renders more neutral colors.
}More than one filter can be stacked in the filter holder (Filter diameter: 45
mm. Maximum thickness of stacked filters: 11 mm).
45IF550-W45
45ND6, 45ND25
45LBD2-N, 45KB
45HA
(Heat absorbing filter)
2
Using the Aperture Iris Diaphragm
70-80%
30-20%
}The aperture iris diaphragm determines the numerical aperture of the
illumination system in brightfield observation. It enables you to adjust the
depth of focus, contrast and resolution according to your requirements.
· Checking the aperture iris diaphragm:
· In general, when observing a dyed specimen, set the aperture iris dia-
phragm to 70% to 80% of the N.A. of the objective in use. However, when
observing a culture specimen, which is not dyed, set the aperture iris
diaphragm lever toward "
3
Removing the Condenser Lens
}To provide more working distance, turn the condenser's lower section 1
in the direction of the arrow and remove it. When you do this, however,
keep in mind that proper illumination cannot be achieved. Remove the
condenser lens only when using a large culture vessel.
Filter
Monochrome contrast filter (Green)
Light intensity adjustment filter
(Transmittance 6% and 25%)
Color temperature conversion filter
(For observation and photomicrography)
Exposure time compensation in photomicrogra-
phy
Remove the eyepiece when necessary (and insert the CT-5 or U-
CT30 if you have one). Then look into the eyepiece sleeve; you
will seen the field of view as shown in Fig. 18. Now adjust the
aperture iris diaphragm lever as required.
".
CKX41/CKX31
Application
(Fig. 18)
(Fig. 19)
15
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