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5.5.3 Monthly Flow Cell
Cleaning
5.6 ELSD Maintenance
5.6.1 Cleaning the ELSD
Detector
EAR99 Technology Technology Subject to Restrictions Contained on the Cover Page
improve. Pump solvent for two to five minutes or until the
indicator reaches the far-right of the green range.
If after five minutes the indicator is still in the red range, repeat
the cleaning steps using an alternative solvent. Or, complete the
steps in the Monthly Flow Cell Cleaning procedure (Section
5.5.3).
Note
If the numerical values of the Raw Lamp Energy (Figure 5-5)
do not change, or if the first number remains at zero, contact
Teledyne ISCO's Technical Service department.
Perform this procedure as part of your scheduled preventive
maintenance, or when Q
5.5.2) does not improve the lamp energy.
1. Remove the column and insert a bypass tube between the
upper and lower column mounts. A bypass tube
(P/N 209-0165-46) can be found in the accessory kit
shipped with your system.
2. From the menu, select T
opens the M
ANUAL
3. Set the Flow Rate to 40 mL/min.
4. Select T
C
HROUGH
5. Select N
for the Pump into Tube # option.
EXT
6. Place the B1 Solvent inlet line into a reservoir of methanol,
acetone, or a strong solvent that readily dissolves residual
sample material.
7. Click the P
B button to pump 100% Solvent B through
RIME
the bypass tube and into the collection tubes.
8. After three minutes, click the S
tem to stand for at least six hours. Overnight is recom-
mended.
9. Return the B1 solvent line to the original solvent container.
10. Perform the Q
UICK
and monitor the Raw Lamp Energy. (Figure 5-5)
If the lamp energy is in the green range, return the system
to operation. If the lamp energy is red, contact Teledyne
ISCO's Technical Service department for assistance.
Periodic cleaning of the spray chamber will keep the NextGen
operating at maximum performance. Use the following steps if
spray chamber cleaning is recommended.
1. From the run screen select the M
2. In the Peak detection window, select the ELS D
radio button then select D
3. Set the spray chamber temperature to
CombiFlash NextGen Systems
Section 5 Maintenance
C
A
R
UICK
LEANING
S
EQUIRED
> M
C
OOLS
ANUAL
C
window.
ONTROL
V
OLUMN FOR THE
ALVE
button. Allow the sys-
TOP
C
R
LEANING AS
EQUIRED
E
ETHOD
.
ETAILS
40 °C
(section
. This
ONTROL
P
option.
OSITION
(Section 5.5.2
.
DITOR
ETECTOR
.
5-7
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